Proteomic Analysis Reveals the Importance of Exudates on Sclerotial Development in Sclerotinia sclerotiorum

A nonsclerotial mutant of Sclerotinia sclerotiorum was produced by mutagenesis with 8-methoxypsoralen and ultraviolet light. The mutant, SL-1, failed to produce sclerotia on artificial media, infested grain, or on infected plants. The mutant remained pathogenic to eight plant species susceptible to the wild-type parental strain of the fungus. The mutant, SL-1, is potentially useful for physiological studies on sclerotial development and for investigation of its potential for biological weed control.Key words: Sclerotinia, mutant, sclerotialess, biocontrol, weeds.

Download Full-text

Sclerotinia sclerotiorum Thioredoxin Reductase Is Required for Oxidative Stress Tolerance, Virulence, and Sclerotial Development

Frontiers in Microbiology ◽

10.3389/fmicb.2019.00233 ◽

2019 ◽

Vol 10 ◽

Cited By ~ 2

Author(s):

Jinyi Zhang ◽

Yabo Wang ◽

Jiao Du ◽

Zhiqiang Huang ◽

Anfei Fang ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Tolerance ◽

Sclerotinia Sclerotiorum ◽

Thioredoxin Reductase ◽

Oxidative Stress Tolerance ◽

Sclerotial Development

Download Full-text

The Sclerotinia sclerotiorum pac1 Gene Is Required for Sclerotial Development and Virulence

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2003.16.9.785 ◽

2003 ◽

Vol 16 (9) ◽

pp. 785-795 ◽

Cited By ~ 181

Author(s):

Jeffrey A. Rollins

Keyword(s):

Oxalic Acid ◽

Sclerotinia Sclerotiorum ◽

Acid Production ◽

Plant Pathogens ◽

Gene Replacement ◽

Loss Of Function ◽

Physiological Processes ◽

Gene Transcripts ◽

Sclerotial Development

The synergistic activities of oxalic acid and endopolygalacturonases are thought to be essential for full virulence of Sclerotinia sclerotiorum and other oxalate-producing plant pathogens. Both oxalic acid production and endopolygalacturonase activity are regulated by ambient pH. Since many gene products with pH-sensitive activities are regulated by the PacC transcription factor in Aspergillus nidulans, we functionally characterized a pacC gene homolog, pac1, from S. sclerotiorum. Mutants with loss-of-function alleles of the pac1 locus were created by targeted gene replacement. In vitro mycelial growth of these pac1 mutants was normal at acidic pH, but growth was inhibited as culture medium pH was increased. Development and maturation of sclerotia in culture was also aberrant in these pac1 replacement mutants. Although oxalic acid production remained alkaline pH-responsive, the kinetics and magnitude of oxalate accumulation were dramatically altered. Additionally, maximal accumulation of endopolygalacturonase gene transcripts (pg1) was shifted to higher ambient pH. Virulence in loss-of-function pac1 mutants was dramatically reduced in infection assays with tomato and Arabidopsis. Based on these results, pac1 appears to be necessary for the appropriate regulation of physiological processes important for pathogenesis and development of S. sclerotiorum.

Download Full-text

Using proteomic analysis to find the proteins involved in resistance against Sclerotinia sclerotiorum in adult Brassica napus

European Journal of Plant Pathology ◽

10.1007/s10658-013-0262-z ◽

2013 ◽

Vol 137 (3) ◽

pp. 505-523 ◽

Cited By ~ 10

Author(s):

Li Wen ◽

Tai-Long Tan ◽

Jia-Bin Shu ◽

Ying Chen ◽

Ying Liu ◽

...

Keyword(s):

Brassica Napus ◽

Sclerotinia Sclerotiorum ◽

Proteomic Analysis

Download Full-text

Polyamine metabolism during sclerotial development of Sclerotinia sclerotiorum

Mycological Research ◽

10.1016/j.mycres.2007.10.016 ◽

2008 ◽

Vol 112 (3) ◽

pp. 414-422 ◽

Cited By ~ 5

Author(s):

Andrés Gárriz ◽

María E. Gonzalez ◽

María Marina ◽

Oscar A. Ruiz ◽

Fernando L. Pieckenstain

Keyword(s):

Sclerotinia Sclerotiorum ◽

Polyamine Metabolism ◽

Sclerotial Development

Download Full-text

Characterization of microRNA-like RNAs associated with sclerotial development in Sclerotinia sclerotiorum

Fungal Genetics and Biology ◽

10.1016/j.fgb.2020.103471 ◽

2020 ◽

Vol 144 ◽

pp. 103471

Author(s):

Zihao Xia ◽

Zehao Wang ◽

Nat N.V. Kav ◽

Chengsong Ding ◽

Yue Liang

Keyword(s):

Sclerotinia Sclerotiorum ◽

Sclerotial Development

Download Full-text

MAPK Regulation of Sclerotial Development in Sclerotinia sclerotiorum Is Linked with pH and cAMP Sensing

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi.2004.17.4.404 ◽

2004 ◽

Vol 17 (4) ◽

pp. 404-413 ◽

Cited By ~ 76

Author(s):

Changbin Chen ◽

Arye Harel ◽

Rena Gorovoits ◽

Oded Yarden ◽

Martin B. Dickman

Keyword(s):

Signaling Pathway ◽

Sclerotinia Sclerotiorum ◽

Specific Inhibitor ◽

Broad Host Range ◽

Environmental Signals ◽

Mitogen Activated Protein ◽

Fungal Phytopathogen ◽

Ph Conditions ◽

Sclerotial Development ◽

Dependent Signaling Pathway

Sclerotial development is fundamental to the disease cycle of the omnivorous broad host range fungal phytopathogen Sclerotinia sclerotiorum. We have isolated a highly conserved homolog of ERK-type mitogen-activated protein kinases (MAPKs) from S. sclerotiorum (Smk1) and have demonstrated that Smk1 is required for sclerotial development. The smk1 transcription and MAPK enzyme activity are induced dramatically during sclerotiogenesis, especially during the production of sclerotial initials. When PD98059 (a specific inhibitor of the activation of MAPK by MAPK kinase) was applied to differentiating cultures or when antisense expression of smk1 was induced, sclerotial maturation was impaired. The smk1 transcript levels were highest under acidic pH conditions, suggesting that Smk1 regulates sclerotial development via a pH-dependent signaling pathway, involving the accumulation of oxalic acid, a previously identified pathogenicity factor that functions at least in part by reducing pH. Addition of cyclic AMP (cAMP) inhibited smk1 transcription, MAPK activation, and sclerotial development. Thus, S. sclerotiorum can coordinate environmental signals (such as pH) to trigger a signaling pathway mediated by Smk1 to induce sclerotia formation, and this pathway is negatively regulated by cAMP.

Download Full-text