Production, Purification, and in Vitro Evaluation of the Prebiotic Potential of Arabinoxylooligosaccharides from Brewer’s Spent Grain

2015 ◽  
Vol 63 (38) ◽  
pp. 8429-8438 ◽  
Author(s):  
Belén Gómez ◽  
Beatriz Míguez ◽  
Adán Veiga ◽  
Juan Carlos Parajó ◽  
José Luís Alonso
Keyword(s):  
In Vitro Evaluation ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Interactions of a Lignin-Rich Fraction from Brewer’s Spent Grain with Gut Microbiota in Vitro

2013 ◽  
Vol 61 (27) ◽  
pp. 6754-6762 ◽  
Author(s):  
Piritta Niemi ◽  
Anna-Marja Aura ◽  
Johanna Maukonen ◽  
Annika I. Smeds ◽  
Ismo Mattila ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Brewer’s Spent Grain Enhanced the Recovery of Potential Probiotic Strains in Fermented Milk After Exposure to In Vitro-Simulated Gastrointestinal Conditions

2021 ◽  
Author(s):  
Carolina Battistini ◽  
Marcos Edgar Herkenhoff ◽  
Marcela de Souza Leite ◽  
Antonio Diogo Silva Vieira ◽  
Raquel Bedani ◽  
...  
Keyword(s):  
Fermented Milk ◽  
Brewer’S Spent Grain ◽  
Gastrointestinal Conditions ◽  
Probiotic Strains ◽  
Spent Grain

scholarly journals Solid-state fermented brewer's spent grain enzymatic extract increases in vitro and in vivo feed digestibility in European seabass

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Helena Fernandes ◽  
Francisco Moyano ◽  
Carolina Castro ◽  
José Salgado ◽  
Francisca Martínez ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
High Potential ◽  
In Vitro Digestibility ◽  
Brewer’S Spent Grain ◽  
Dietary Phosphorus ◽  
European Seabass ◽  
Spent Grain

AbstractBrewer’s spent grain (BSG) is the largest by-product originated from the brewery industry with a high potential for producing carbohydrases by solid-state fermentation. This work aimed to test the efficacy of a carbohydrases-rich extract produced from solid-state fermentation of BSG, to enhance the digestibility of a plant-based diet for European seabass (Dicentrarchus labrax). First, BSG was fermented with A. ibericus to obtain an aqueous lyophilized extract (SSF-BSG extract) and incorporated in a plant-based diet at increasing levels (0—control; 0.1%, 0.2%, and 0.4%). Another diet incorporating a commercial carbohydrases-complex (0.04%; Natugrain; BASF) was formulated. Then, all diets were tested in in vitro and in vivo digestibility assays. In vitro assays, simulating stomach and intestine digestion in European seabass, assessed dietary phosphorus, phytate phosphorus, carbohydrates, and protein hydrolysis, as well as interactive effects between fish enzymes and dietary SSF-BSG extract. After, an in vivo assay was carried out with European seabass juveniles fed selected diets (0—control; 0.1%, and 0.4%). In vitro digestibility assays showed that pentoses release increased 45% with 0.4% SSF-BSG extract and 25% with Natugrain supplemented diets, while amino acids release was not affected. A negative interaction between endogenous fish enzymes and SSF-BSG extract was observed in both diets. The in vivo digestibility assay corroborated in vitro data. Accordingly, the dietary supplementation with 0.4% SSF-BSG increased the digestibility of dry matter, starch, cellulose, glucans, and energy and did not affect protein digestibility. The present work showed the high potential of BSG to produce an added-value functional supplement with high carbohydrases activity and its potential contribution to the circular economy by improving the nutritional value of low-cost and sustainable ingredients that can be included in aquafeeds.

scholarly journals In vitro Rumen Degradability Study of Wet Brewer’s Spent Grain by Modified Rumen Simulation Technique (TANUVAS-RUSITECTM)

2020 ◽  
Vol 9 (5) ◽  
pp. 1118-1127
Author(s):  
V. Thavasiappan ◽  
K. Nanjappan ◽  
P. Visha ◽  
R. Ezakial Napolean ◽  
P. Selvaraj ◽  
...  
Keyword(s):  
Simulation Technique ◽  
Brewer’S Spent Grain ◽  
Spent Grain

Release of Small Phenolic Compounds from Brewer’s Spent Grain and Its Lignin Fractions by Human Intestinal Microbiota in Vitro

2013 ◽  
Vol 61 (40) ◽  
pp. 9744-9753 ◽  
Author(s):  
Anna-Marja Aura ◽  
Piritta Niemi ◽  
Ismo Mattila ◽  
Klaus Niemelä ◽  
Annika Smeds ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Intestinal Microbiota ◽  
Brewer’S Spent Grain ◽  
Human Intestinal Microbiota ◽  
Spent Grain

Protein Hydrolysate from Brewer’s Spent Grain and its Inhibitory Ability of α-Glucosidase

2012 ◽  
Vol 581-582 ◽  
pp. 138-141 ◽  
Author(s):  
Hong Jia Lin ◽  
Lin Li ◽  
Ying Juan Tian ◽  
Xia Zhang ◽  
Bing Li
Keyword(s):  
Molecular Weight ◽  
Protein Hydrolysate ◽  
Weight Fraction ◽  
Hydrolysis Time ◽  
Brewer’S Spent Grain ◽  
Inhibitory Ability ◽  
Spent Grain ◽  
By Products

Brewer’s spent grain (BSG) rich in proteins is the main by-products in brewery. BSC proteins were extracted and hydrolyzed by Alcalase. The effects of hydrolysis time, temperature, pH and amount of enzyme added on the peptide yield were investigated. Inhibition of BSG protein hydrolysate against α-glucosidase was evaluated in vitro. BSG protein hydrolysate at 4.0 mg/mL showed the inhibition of 21.42% against α-glucosidase. The BSG protein hydrolysate after ultrafiltration purification exhibited much higher inhibition than that without ultrafiltration purification. The inhibition of BSC protein hydrolysate fraction with molecular weight fraction less than 5 kDa was 56.41%.

scholarly journals In vitro comparison of hydrolysis of different starches along the digestive tract of teleosts important for aquaculture

2017 ◽  
Vol 57 (3) ◽  
pp. 205
Author(s):  
E. S. PAPOUTSOGLOU (Ε.Σ. ΠΑΠΟΥΤΣΟΓΛΟΥ) ◽  
A. R. LYNDON
Keyword(s):  
Sparus Aurata ◽  
Amylose Content ◽  
Sea Bream ◽  
European Sea Bass ◽  
Carbohydrate Source ◽  
Brewer’S Spent Grain ◽  
Significant Difference ◽  
Spent Grain ◽  
Hydrolysis Of

The in vitro hydrolysis of different starches (potato, rice, wheat, corn, sorghum), as well as an intermediate product of malting process (brewer's spent grain), by blue tilapia, Oreochromis aureus, gilthead sea bream, Sparus aurata and European sea bass, Dicentrarchus labrax, were compared (total carbohydrase assay, 37° C, pH 7.6,4 hours incubation). Obtained results (significandy higher hydrolysis levels obtained for potato, rice and sorghum starch) suggest that starch source and associated properties (starch granule size, amylose content) and modifications applied may have significant difference on its digestion by fish digestive carbohydrases. Furthermore, the importance of brewer's spent grain as a low-cost carbohydrate source and feed component, in relation to aglucosidase adaptive response to dietary carbohydrate, is also considered significant.

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