Fast Determination of Phenolic Compounds in Rice Grains by Ultraperformance Liquid Chromatography Coupled to Photodiode Array Detection: Method Development and Validation

A new sample preparation and chromatographic separation method for methyl, propyl and butyl paraben in cosmetic and environmental samples has been developed, based on fabric phase sorptive extraction and high performance liquid chromatography-photodiode array detection.

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Capillary electrophoresis with capacitively coupled contactless conductivity detection method development and validation for the determination of azithromycin, clarithromycin, and clindamycin

Journal of Separation Science ◽

10.1002/jssc.201700560 ◽

2017 ◽

Vol 40 (17) ◽

pp. 3535-3544 ◽

Cited By ~ 14

Author(s):

Prasanta Paul ◽

Tom Duchateau ◽

Cari Sänger-van de Griend ◽

Erwin Adams ◽

Ann Van Schepdael

Keyword(s):

Capillary Electrophoresis ◽

Method Development ◽

Detection Method ◽

Conductivity Detection ◽

Capacitively Coupled ◽

Contactless Conductivity Detection ◽

Method Development And Validation ◽

Development And Validation

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Method Development and Validation of LC and Densitometric-TLC for Simultaneous Determination of Pitavastatin Calcium and Ezetimibe in a Binary Mixture

Journal of AOAC International ◽

10.5740/jaoacint.11-075 ◽

2014 ◽

Vol 97 (1) ◽

pp. 99-104 ◽

Cited By ~ 1

Author(s):

Hiral J Panchal ◽

Bhanubhai N Suhagia

Keyword(s):

Binary Mixture ◽

Method Development ◽

Array Detector ◽

Photodiode Array Detector ◽

Method Development And Validation ◽

Rf Values ◽

Photodiode Array ◽

Pitavastatin Calcium ◽

Development And Validation

Abstract Two simple and accurate methods to determine pitavastatin calcium (PIT) and ezetimibe (EZE), ina binary mixture, were developed and validated using LC and densitometric-TLC. The LC separation was achieved on a Phenomenex Luna C18 column (250 × 4.6 mm id, 5 μm), in the isocratic mode using 0.1% orthophosphoric acid–acetonitrile–triethylamine (19.8 + 80 + 0.2, v/v/v), pH 3.00 ± 0.05, at a flow rate of 1.4 mL/min. The retention times were about 6.98 and 2.36 min for PIT and EZE, respectively. Quantification was achieved with a photodiode array detector at 235 nm over the concentration range of 0.5–5 μg/mL for each drug, with meanrecoveries of 99.35 ± 0.19% and 99.51 ± 0.23% for PIT and EZE, respectively. The TLCseparation was achieved on silica gel 60 F254 HPTLC plates using toluene–ethyl acetate–glacial acetic acid (59.8 + 40.0 + 0.2, v/v/v), as the mobile phase. The Rf values were about 0.38 and 0.58 for PIT and EZE, respectively. Quantification was achieved withUV detection at 235 nm over the concentration range of 50–600 ng/spot for each drug, with mean recoveries of 99.49 ± 0.15% and 99.58 ± 0.10% for PIT and EZE, respectively. Both methods were validated, and based on statistical results were found to be specific, accurate, precise, and robust. The methods were successfully applied for the determination of PIT and EZE in abinary mixture without any interferences from common excipients.

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