Solvatochromic Fluorescence of a GFP Chromophore-Containing Organogelator in Solutions and Organogels

2021 ◽  
Author(s):  
Meng-Shiue Tsai ◽  
Chin-Han Lee ◽  
Jye-Chian Hsiao ◽  
Shih-Sheng Sun ◽  
Jye-Shane Yang
Keyword(s):  
Gfp Chromophore

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

2020 ◽  
Vol 124 (5) ◽  
pp. 871-880 ◽  
Author(s):  
Hongping Deng ◽  
Chunyang Yu ◽  
Deyue Yan ◽  
Xinyuan Zhu
Keyword(s):  
Gfp Chromophore

The Case of the Missing Ring:  Radical Cleavage of a Carbon−Carbon Bond and Implications for GFP Chromophore Biosynthesis

2007 ◽  
Vol 129 (11) ◽  
pp. 3118-3126 ◽  
Author(s):  
David P. Barondeau ◽  
Carey J. Kassmann ◽  
John A. Tainer ◽  
Elizabeth D. Getzoff
Keyword(s):  
Carbon Bond ◽  
Carbon Carbon Bond ◽  
Gfp Chromophore ◽  
Radical Cleavage ◽  
Missing Ring

scholarly journals Communication: Autodetachment versus internal conversion from the S1 state of the isolated GFP chromophore anion

2013 ◽  
Vol 139 (7) ◽  
pp. 071104 ◽  
Author(s):  
Christopher W. West ◽  
Alex S. Hudson ◽  
Steven L. Cobb ◽  
Jan R. R. Verlet
Keyword(s):  
Internal Conversion ◽  
Gfp Chromophore

Fluorescent and “breathable” CO2 responsive vesicles inspired from green fluorescent protein

2017 ◽  
Vol 8 (40) ◽  
pp. 6283-6288 ◽  
Author(s):  
Lei Xu ◽  
Ning Ren ◽  
Ji Pang ◽  
Hongping Deng ◽  
Xinyuan Zhu ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Block Copolymer ◽  
Fluorescent Protein ◽  
Gfp Chromophore ◽  
Green Fluorescent

CO2 responsive fluorescent vesicles from a GFP chromophore labeled block-copolymer could change their size and fluorescence to mimic jellyfish breathing.

scholarly journals Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins

2018 ◽  
Vol 19 (12) ◽  
pp. 3778 ◽  
Author(s):  
Nina Bozhanova ◽  
Mikhail Baranov ◽  
Nadezhda Baleeva ◽  
Alexey Gavrikov ◽  
Alexander Mishin
Keyword(s):  
Green Fluorescent Protein ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Fluorescent Protein ◽  
Live Cell ◽  
Protein Labeling ◽  
Cell Protein ◽  
Gfp Chromophore ◽  
Green Fluorescent ◽  
Derivatives Of

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs.

Environment-sensitive fluorogens based on a GFP chromophore structural motif

2021 ◽  
pp. 110033
Author(s):  
Maxim M. Perfilov ◽  
Elvira R. Zaitseva ◽  
Alexander Yu Smirnov ◽  
Andrey A. Mikhaylov ◽  
Nadezhda S. Baleeva ◽  
...  
Keyword(s):  
Structural Motif ◽  
Gfp Chromophore

Delayed vibrational modulation of the solvated GFP chromophore into a conical intersection

2019 ◽  
Vol 21 (19) ◽  
pp. 9728-9739 ◽  
Author(s):  
Miles A. Taylor ◽  
Liangdong Zhu ◽  
Nikita D. Rozanov ◽  
Kenneth T. Stout ◽  
Cheng Chen ◽  
...  
Keyword(s):  
Conical Intersection ◽  
Gfp Chromophore

Upon photoexcitation, a delayed structural bending motion facilitates the “dark” GFP chromophore ring-twisting isomerization in reaching a conical intersection in solution.

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