scholarly journals Deep Time Course Proteomics of SARS-CoV- and SARS-CoV-2-Infected Human Lung Epithelial Cells (Calu-3) Reveals Strong Induction of Interferon-Stimulated Gene Expression by SARS-CoV-2 in Contrast to SARS-CoV

2022 ◽  
Author(s):  
Marica Grossegesse ◽  
Daniel Bourquain ◽  
Markus Neumann ◽  
Lars Schaade ◽  
Jessica Schulze ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Time Course ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Deep Time ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

scholarly journals Gene Expression Profiling Reveals the Shared and Distinct Transcriptional Signatures in Human Lung Epithelial Cells Infected With SARS-CoV-2, MERS-CoV, or SARS-CoV: Potential Implications in Cardiovascular Complications of COVID-19

2021 ◽  
Vol 7 ◽  
Author(s):  
Prabhash Kumar Jha ◽  
Aatira Vijay ◽  
Arda Halu ◽  
Shizuka Uchida ◽  
Masanori Aikawa
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Severe Acute Respiratory Syndrome ◽  
Vascular Function ◽  
Human Lung ◽  
Cardiovascular Complications ◽  
Lung Epithelial Cells ◽  
Upper Respiratory Tract ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative virus for the current global pandemic known as coronavirus disease 2019 (COVID-19). SARS-CoV-2 belongs to the family of single-stranded RNA viruses known as coronaviruses, including the MERS-CoV and SARS-CoV that cause Middle East respiratory syndrome (MERS) and severe acute respiratory syndrome (SARS), respectively. These coronaviruses are associated in the way that they cause mild to severe upper respiratory tract illness. This study has used an unbiased analysis of publicly available gene expression datasets from Gene Expression Omnibus to understand the shared and unique transcriptional signatures of human lung epithelial cells infected with SARS-CoV-2 relative to MERS-CoV or SARS-CoV. A major goal was to discover unique cellular responses to SARS-CoV-2 among these three coronaviruses. Analyzing differentially expressed genes (DEGs) shared by the three datasets led to a set of 17 genes, suggesting the lower expression of genes related to acute inflammatory response (TNF, IL32, IL1A, CXCL1, and CXCL3) in SARS-CoV-2. This subdued transcriptional response to SARS-CoV-2 may cause prolonged viral replication, leading to severe lung damage. Downstream analysis of unique DEGs of SARS-CoV-2 infection revealed changes in genes related to apoptosis (NRP1, FOXO1, TP53INP1, CSF2, and NLRP1), coagulation (F3, PROS1, ITGB3, and TFPI2), and vascular function (VAV3, TYMP, TCF4, and NR2F2), which may contribute to more systemic cardiovascular complications of COVID-19 than MERS and SARS. The study has uncovered a novel set of transcriptomic signatures unique to SARS-CoV-2 infection and shared by three coronaviruses, which may guide the initial efforts in the development of prognostic or therapeutic tools for COVID-19.

scholarly journals Differences in gene expression and cytokine production by crystalline vs. amorphous silica in human lung epithelial cells

2012 ◽  
Vol 9 (1) ◽  
pp. 6 ◽  
Author(s):  
Timothy N Perkins ◽  
Arti Shukla ◽  
Paul M Peeters ◽  
Jeremy L Steinbacher ◽  
Christopher C Landry ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Amorphous Silica ◽  
Cytokine Production ◽  
Human Lung ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

scholarly journals Global Gene Expression Profiling of Human Lung Epithelial Cells After Exposure to Nanosilver

2012 ◽  
Vol 130 (1) ◽  
pp. 145-157 ◽  
Author(s):  
Rasmus Foldbjerg ◽  
Eveline S. Irving ◽  
Yuya Hayashi ◽  
Duncan S. Sutherland ◽  
Kasper Thorsen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Human Lung ◽  
Global Gene Expression ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Global Gene Expression Profiling ◽  
Human Lung Epithelial Cells

scholarly journals Identification of biomarkers and pathways for the SARS-CoV-2 infections that make complexities in pulmonary arterial hypertension patients

2021 ◽  
Author(s):  
Tasnimul Alam Taz ◽  
Kawsar Ahmed ◽  
Bikash Kumar Paul ◽  
Fahad Ahmed Al-Zahrani ◽  
S M Hasan Mahmud ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pulmonary Arterial Hypertension ◽  
Epithelial Cells ◽  
Arterial Hypertension ◽  
Human Lung ◽  
Genomic Analysis ◽  
Lung Epithelial Cells ◽  
Lung Epithelial ◽  
Pulmonary Arterial ◽  
Human Lung Epithelial Cells

Abstract This study aimed to identify significant gene expression profiles of the human lung epithelial cells caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We performed a comparative genomic analysis to show genomic observations between SARS-CoV and SARS-CoV-2. A phylogenetic tree has been carried for genomic analysis that confirmed the genomic variance between SARS-CoV and SARS-CoV-2. Transcriptomic analyses have been performed for SARS-CoV-2 infection responses and pulmonary arterial hypertension (PAH) patients’ lungs as a number of patients have been identified who faced PAH after being diagnosed with coronavirus disease 2019 (COVID-19). Gene expression profiling showed significant expression levels for SARS-CoV-2 infection responses to human lung epithelial cells and PAH lungs as well. Differentially expressed genes identification and integration showed concordant genes (SAA2, S100A9, S100A8, SAA1, S100A12 and EDN1) for both SARS-CoV-2 and PAH samples, including S100A9 and S100A8 genes that showed significant interaction in the protein–protein interactions network. Extensive analyses of gene ontology and signaling pathways identification provided evidence of inflammatory responses regarding SARS-CoV-2 infections. The altered signaling and ontology pathways that have emerged from this research may influence the development of effective drugs, especially for the people with preexisting conditions. Identification of regulatory biomolecules revealed the presence of active promoter gene of SARS-CoV-2 in Transferrin-micro Ribonucleic acid (TF-miRNA) co-regulatory network. Predictive drug analyses provided concordant drug compounds that are associated with SARS-CoV-2 infection responses and PAH lung samples, and these compounds showed significant immune response against the RNA viruses like SARS-CoV-2, which is beneficial in therapeutic development in the COVID-19 pandemic.

Microarray analysis of Streptococcus pneumoniae gene expression changes to human lung epithelial cells

2008 ◽  
Vol 54 (3) ◽  
pp. 189-200 ◽  
Author(s):  
Xin-Ming Song ◽  
Wayne Connor ◽  
Shakiba Jalal ◽  
Karsten Hokamp ◽  
Andrew A. Potter
Keyword(s):  
Gene Expression ◽  
Streptococcus Pneumoniae ◽  
Epithelial Cells ◽  
Human Lung ◽  
Cell Envelope ◽  
A549 Cells ◽  
Lung Epithelial Cells ◽  
Pcr Analysis ◽  
Lung Epithelial ◽  
Human Lung Epithelial Cells

Streptococcus pneumoniae infection starts from the respiratory tract where interaction with host epithelial cells occurs. To gain more insights on pneumococcal pathogenesis, an oligonucleotide (oligo)-based microarray was used to investigate gene expression changes of one serotype 3 encapsulated pathogenic S. pneumoniae strain 82 and one unencapsulated avirulent S. pneumoniae strain R6 upon exposure to human lung epithelial cells (A549) for 1 and 3 h, respectively. We observed that genes associated with many functional categories were differentially regulated in strain 82, such as genes in pathogenesis, cell envelope, transcription, translation, transport, metabolism, and unknown functions. In contrast, few genes were changed in strain R6 except for genes in ribonucleotide biosynthesis and unknown functions. Quantitative real-time PCR analysis confirmed the microarray results for most of the genes tested. To further characterize functions of the selected genes, knockout mutants were constructed in strain R6. We demonstrated that 2 genetic loci, SP_2170 (AdcB, zinc ABC transporter) and SP_0157 (hypothetical protein), were involved in adherence to A549 cells. These data suggest that divergent gene expression changes occur in S. pneumoniae pathogenic and avirulent strains during interaction with human lung epithelial cells. Some of those genes are involved in pneumococcal pathogenesis.

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