Impact of Oxygen Concentration on Metabolic Profile of Human Placenta-Derived Mesenchymal Stem Cells As Determined by Chemical Isotope Labeling LC–MS

2018 ◽  
Vol 17 (5) ◽  
pp. 1866-1878 ◽  
Author(s):  
Dan Wang ◽  
Deying Chen ◽  
Jiong Yu ◽  
Jingqi Liu ◽  
Xiaowei Shi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Oxygen Concentration ◽  
Human Placenta ◽  
Metabolic Profile ◽  
Isotope Labeling ◽  
Chemical Isotope Labeling

scholarly journals Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface

2016 ◽  
Vol 2016 ◽  
pp. 1-16 ◽  
Author(s):  
Tzyy Yue Wong ◽  
Ying-Hui Chen ◽  
Szu-Heng Liu ◽  
Mairim Alexandra Solis ◽  
Chen-Hsiang Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Tissue Culture ◽  
Mesenchymal Stem Cells ◽  
Human Placenta ◽  
Normal Tissue ◽  
Cell Function ◽  
Isotope Labeling ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Coated Surface ◽  
Culture Surface

Our previous results showed that hyaluronan (HA) preserved human placenta-derived mesenchymal stem cells (PDMSC) in a slow cell cycling mode similar to quiescence, the pristine state of stem cellsin vivo, and HA was found to prevent murine adipose-derived mesenchymal stem cells from senescence. Here, stable isotope labeling by amino acid in cell culture (SILAC) proteomic profiling was used to evaluate the effects of HA on aging phenomenon in stem cells, comparing (1) old and young passage PDMSC cultured on normal tissue culture surface (TCS); (2) old passage on HA-coated surface (CHA) compared to TCS; (3) old and young passage on CHA. The results indicated that senescence-associated protein transgelin (TAGLN) was upregulated in old TCS. Protein CYR61, reportedly senescence-related, was downregulated in old CHA compared to old TCS. The SIRT1-interacting Nicotinamide phosphoribosyltransferase (NAMPT) increased by 2.23-fold in old CHA compared to old TCS, and is 0.48-fold lower in old TCS compared to young TCS. Results also indicated that components of endoplasmic reticulum associated degradation (ERAD) pathway were upregulated in old CHA compared to old TCS cells, potentially for overcoming stress to maintain cell function and suppress senescence. Our data points to pathways that may be targeted by HA to maintain stem cells youth.

scholarly journals Osteogenic differentiation of human placenta-derived mesenchymal stem cells (PMSCs) on electrospun nanofiber meshes

2012 ◽  
Vol 64 (6) ◽  
pp. 701-710 ◽  
Author(s):  
Dongmei Zhang ◽  
Aiping Tong ◽  
Liangxue Zhou ◽  
Fang Fang ◽  
Gang Guo
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Human Placenta ◽  
Electrospun Nanofiber

Human placenta‐derived mesenchymal stem cells inhibit apoptosis of granulosa cells induced by IRE1α pathway in autoimmune POF mice

2019 ◽  
Vol 43 (8) ◽  
pp. 899-909 ◽  
Author(s):  
Hongxing Li ◽  
Wei Zhao ◽  
Li Wang ◽  
Qianqian Luo ◽  
Na Yin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Granulosa Cells ◽  
Human Placenta

Expression and biological function of programmed death ligands in human placenta mesenchymal stem cells

2013 ◽  
Vol 37 (2) ◽  
pp. 137-148 ◽  
Author(s):  
Guoyan Wang ◽  
Siying Zhang ◽  
Feifei Wang ◽  
Guangyun Li ◽  
Lixia Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Placenta ◽  
Biological Function ◽  
Programmed Death
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