scholarly journals Detailed Analysis of the Ice Surface after Binding of an Insect Antifreeze Protein and Correlation with the Gibbs–Thomson Equation

Langmuir ◽  
2021 ◽  
Vol 37 (40) ◽  
pp. 11716-11725
Author(s):  
Julian Gerhäuser ◽  
Volker Gaukel
Keyword(s):  
Detailed Analysis ◽  
Antifreeze Protein ◽  
Ice Surface

Ordered hydration layer mediated ice adsorption of a globular antifreeze protein: mechanistic insight

2019 ◽  
Vol 21 (35) ◽  
pp. 19298-19310 ◽  
Author(s):  
Sandipan Chakraborty ◽  
Biman Jana
Keyword(s):  
Antifreeze Protein ◽  
Hydration Layer ◽  
Type Iii ◽  
Ice Surface ◽  
Mechanistic Insight ◽  
Ice Binding ◽  
Binding Surface ◽  
Lower Temperature

The ice binding surface of a type III AFP induces water ordering at lower temperature, which mediates its adsorption on the ice surface.

scholarly journals Preordering of water is not needed for ice recognition by hyperactive antifreeze proteins

2018 ◽  
Vol 115 (33) ◽  
pp. 8266-8271 ◽  
Author(s):  
Arpa Hudait ◽  
Daniel R. Moberg ◽  
Yuqing Qiu ◽  
Nathan Odendahl ◽  
Francesco Paesani ◽  
...  
Keyword(s):  
Binding Site ◽  
Raman Spectra ◽  
Antifreeze Proteins ◽  
Antifreeze Protein ◽  
Bulk Liquid ◽  
Cold Environments ◽  
Ice Surface ◽  
Ice Binding ◽  
Binding Surface ◽  
Optimal Candidate

Antifreeze proteins (AFPs) inhibit ice growth in organisms living in cold environments. Hyperactive insect AFPs are particularly effective, binding ice through “anchored clathrate” motifs. It has been hypothesized that the binding of hyperactive AFPs to ice is facilitated by preordering of water at the ice-binding site (IBS) of the protein in solution. The antifreeze proteinTmAFP displays the best matching of its binding site to ice, making it the optimal candidate to develop ice-like order in solution. Here we use multiresolution simulations to unravel the mechanism by whichTmAFP recognizes and binds ice. We find that water at the IBS of the antifreeze protein in solution does not acquire ice-like or anchored clathrate-like order. Ice recognition occurs by slow diffusion of the protein to achieve the proper orientation with respect to the ice surface, followed by fast collective organization of the hydration water at the IBS to form an anchored clathrate motif that latches the protein to the ice surface. The simulations suggest that anchored clathrate order could develop on the large ice-binding surfaces of aggregates of ice-nucleating proteins (INP). We compute the infrared and Raman spectra of water in the anchored clathrate motif. The signatures of the OH stretch of water in the anchored clathrate motif can be distinguished from those of bulk liquid in the Raman spectra, but not in the infrared spectra. We thus suggest that Raman spectroscopy may be used to probe the anchored clathrate order at the ice-binding surface of INP aggregates.

The Effects of Small System on the Type I Antifreeze Protein ‘HPLC-6’

2013 ◽  
Vol 275-277 ◽  
pp. 1615-1618
Author(s):  
Li Fen Li ◽  
Xi Xia Liang
Keyword(s):  
Antifreeze Protein ◽  
Type I ◽  
Small System ◽  
Ice Surface

According to Hill’s thermodynamics theory for small system, the effect of small system on the type I antifreeze protein ‘HPLC-6’ is discussed in this article. We conclude that when the solution is very dilute, the effect is not visible, and as the concentration increases, the effect becomes more visible than before, and the result also shows that the number of molecules on the ice surface becomes larger when the effect of small system is considered.

Discrimination of monomer from multimer DNA disk-shaped toruses by freezeetch TEM

1984 ◽  
Vol 42 ◽  
pp. 210-211
Author(s):  
George C. Ruben ◽  
Kenneth A. Marx
Keyword(s):  
Biochemical Data ◽  
Dna Structures ◽  
Ice Surface ◽  
Double Stranded Dna ◽  
Data Support ◽  
Dna Organization ◽  
Trivalent Cations

In vitro collapse of DNA by trivalent cations like spermidine produces torus (donut) shaped DNA structures thought to have a DNA organization similar to certain double stranded DNA bacteriophage and viruses. This has prompted our studies of these structures using freeze-etch low Pt-C metal (9Å) replica TEM. With a variety of DNAs the TEM and biochemical data support a circumferential DNA winding model for hydrated DNA torus organization. Since toruses are almost invariably oriented nearly horizontal to the ice surface one of the most accessible parameters of a torus population is annulus (ring) thickness. We have tabulated this parameter for populations of both nicked, circular (Fig. 1: n=63) and linear (n=40: data not shown) ϕX-174 DNA toruses. In both cases, as can be noted in Fig. 1, there appears to be a compact grouping of toruses possessing smaller dimensions separated from a dispersed population possessing considerably larger dimensions.

Prosodic Impairment Associated With Traumatic Brain Injury

2009 ◽  
Vol 19 (3) ◽  
pp. 97-102
Author(s):  
Billy Irwin
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Detailed Analysis ◽  
Acoustic Characteristics ◽  
Individual Variations

Abstract Purpose: This article discusses impaired prosody production subsequent to traumatic brain injury (TBI). Prosody may affect naturalness and intelligibility of speech significantly, often for the long term, and TBI may result in a variety of impairments. Method: Intonation, rate, and stress production are discussed in terms of the perceptual, physiological, and acoustic characteristics associated with TBI. Results and Conclusions: All aspects of prosodic production are susceptible to the effects of damage resulting from TBI. There are commonly associated prosodic impairments; however, individual variations in specific aspects of prosody require detailed analysis.

ELLIPSOMETRIC STUDY OF THE ICE SURFACE STRUCTURE JUST BELOW THE MELTING POINT

1987 ◽  
Vol 48 (C1) ◽  
pp. C1-495-C1-501 ◽  
Author(s):  
Y. FURUKAWA ◽  
M. YAMAMOTO ◽  
T. KURODA
Keyword(s):  
Melting Point ◽  
Surface Structure ◽  
Ice Surface ◽  
Ellipsometric Study
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