Control Release Coating for Urinary Catheters with Enhanced Released Profile for Sustained Antimicrobial Protection

SummaryIn endothelial cells von Willebrand factor (vWF) and P-selectin are stored in dense granules, so-called Weibel-Palade bodies. Upon stimulation of endothelial cells with a variety of agents including thrombin, these organelles fuse with the plasma membrane and release their content. Small GTP-binding proteins have been shown to control release from intracellular storage pools in a number of cells. In this study we have investigated whether small GTP-binding proteins are associated with Weibel-Palade bodies. We isolated Weibel-Palade bodies by centrifugation on two consecutive density gradients of Percoll. The dense fraction in which these subcellular organelles were highly enriched, was analysed by SDS-PAGE followed by GTP overlay. A distinct band with an apparent molecular weight of 28,000 was observed. Two-dimensional gel electrophoresis followed by GTP overlay revealed the presence of a single small GTP-binding protein with an isoelectric point of 7.1. A monoclonal antibody directed against RalA showed reactivity with the small GTP-binding protein present in subcellular fractions that contain Weibel-Palade bodies. The small GTPase RalA was previously identified on dense granules of platelets and on synaptic vesicles in nerve terminals. Our observations suggest that RalA serves a role in regulated exocytosis of Weibel-Palade bodies in endothelial cells.

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NATURAL COSMETICS IN THE CARE OF YOUNG CHILDREN

PEDIATRIA Journal named after G N SPERANSKY ◽

10.24110/0031-403x-2020-99-6-155-162 ◽

2020 ◽

Vol 99 (6) ◽

pp. 155-162

Author(s):

O.B. Tamrazova ◽

◽

S.P. Seleznev ◽

A.V. Tamrazova ◽

◽

...

Keyword(s):

Skin Barrier ◽

Structural Features ◽

Transepidermal Water Loss ◽

General Information ◽

Skin Barrier Function ◽

Skin Physiology ◽

Main Emphasis ◽

Antimicrobial Protection ◽

Irritant Dermatitis ◽

Adaptive Shifts

The article provides general information about the skin physiology of newborns and infants. Structural features of the skin and main adaptive shifts in newborns, are described. Тhe child has an increase in the skin barrier function of the skin, which prevents transepidermal water loss; active synthesis of natural moisturizing factor (NMF) components that control skin hydration; shift of pH to acidic environment; normalization of thermoregulatory functions; enhancement of the photoprotective function; immune restructuring for antimicrobial protection; formation of a normal microbiome. The article describes the consequences of improper skin care of a newborn, using the example of diaper dermatitis, irritant dermatitis, prickly heat and vesiculopustulosis. The importance of using specialized children's cosmetics in caring for an infant is assessed. The basic recommendations for the choice of these products are presented, where the main emphasis is on the choice of products consisting of natural ingredients. Giving preference to natural cosmetics, everyone should carefully study the composition of these products and trust the manufacturers who can guarantee safety of care products for the youngest children.

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Faculty Opinions recommendation of Urinary catheters. Part 1--male catheterisation.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1141961.599073 ◽

2009 ◽

Author(s):

Yong-Hyun Cho

Keyword(s):

Urinary Catheters

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Urinary Catheters and Biofilm Formation

Recent Patents on Biomarkerse ◽

10.2174/22103090113036660010 ◽

2013 ◽

Vol 3 (3) ◽

pp. 196-203 ◽

Cited By ~ 1

Author(s):

Daniele Minardi ◽

Alessandro Conti ◽

Matteo Santoni ◽

Daniele Cantoro ◽

Oscar Cirioni ◽

...

Keyword(s):

Biofilm Formation ◽

Urinary Catheters

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Evaluation of the Antimicrobial Protection of Pharmaceutical Kaolin and Talc Modified with Copper and Zinc

Materials ◽

10.3390/ma14051173 ◽

2021 ◽

Vol 14 (5) ◽

pp. 1173

Author(s):

Fotini Martsouka ◽

Konstantinos Papagiannopoulos ◽

Sophia Hatziantoniou ◽

Martin Barlog ◽

Giorgos Lagiopoulos ◽

...

Keyword(s):

Cation Exchange ◽

Photoelectron Spectroscopy ◽

Antimicrobial Properties ◽

Exchange Capacity ◽

Elevated Concentration ◽

Chemical Mapping ◽

Exchange Method ◽

X Ray ◽

Copper And Zinc ◽

Antimicrobial Protection

Six pharmaceutical pastes were prepared using chemically modified kaolin and talc powders. Tests were conducted to determine their structural and chemical characteristics as well as their antimicrobial protection, thus rendering them suitable for cosmetic and pharmaceutical uses. Kaolin and talc were treated chemically via the cation exchange method to load the clay particles with copper and zinc ions, two cations well known for their antimicrobial properties. Mineralogical analyses were conducted by using X-ray diffraction (XRD) before and after the modification, confirming the mineralogical purity of the samples. Scanning electron microscopy was also used in conjunction with energy dispersed spectroscopy (SEM-EDS) to obtain chemical mapping images, revealing the dispersion of the added metals upon the clay minerals surfaces. Moreover, chemical analysis has been performed (XRF) to validate the enrichment of the clays with each metal utilizing the cation exchange capacity. All modified samples showed the expected elevated concentration in copper or zinc in comparison to their unmodified versions. From the X-ray photoelectron spectroscopy (XPS), the chemical state of the samples’ surfaces was investigated, revealing the presence of salt compounds and indicating the oxidation state of adsorbed metals. Finally, the resistance of pastes in microbial growth when challenged with bacteria, molds, and yeasts was assessed. The evaluation is based on the European Pharmacopeia (EP) criteria.

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Differences among institutions in the prevalence of and indications for urinary catheterization of advanced cancer patients at palliative care units: A multicenter prospective cohort study (EASED)

Palliative Medicine ◽

10.1177/0269216321989564 ◽

2021 ◽

pp. 026921632198956

Author(s):

Takahiro Higashibata ◽

Takayuki Hisanaga ◽

Shingo Hagiwara ◽

Miho Shimokawa ◽

Ritsuko Yabuki ◽

...

Keyword(s):

Palliative Care ◽

Cancer Patients ◽

Advanced Cancer ◽

Urinary Catheterization ◽

Urinary Catheters ◽

Advanced Cancer Patients ◽

Appropriate Use ◽

Palliative Care Units ◽

Multicenter Prospective Cohort Study ◽

Multicenter Prospective Cohort

Background: Studies on the appropriate use of urinary catheters for cancer patients at the end of life are limited. Aim: To clarify the differences among institutions in the prevalence of and indications for urinary catheterization of advanced cancer patients at palliative care units. Design: Pre-planned secondary analysis of a multicenter, prospective cohort study; East-Asian collaborative cross-cultural Study to Elucidate the Dying process (EASED). Setting/participants: This study enrolled consecutive advanced cancer patients admitted to palliative care units between January and December 2017. The final study group comprised 1212 patients from 21 institutions throughout Japan. Results: Out of the 1212 patients, 380 (31.4%; 95% confidence interval, 28.7%–34.0%) underwent urinary catheterization during their palliative care unit stay, and the prevalence of urinary catheterization in patients who died at palliative care units by institution ranged from 0.0% to 55.4%. When the 21 participating institutions were equally divided into three groups according to the institutional prevalence of catheterization, patients with difficulty in moving safely, exhaustion on movement, and restlessness or agitation were more likely to be catheterized in institutions with a high prevalence of catheterization than in those with a low or moderate prevalence ( p < 0.008, p = 0.008, and p < 0.008, respectively). Conclusion: This study revealed that the institutional prevalence of urinary catheterization in advanced cancer patients at palliative care units widely varied. Further studies are needed to establish the appropriate use of urinary catheters, especially in patients with difficulty in moving safely, exhaustion on movement, and restlessness or agitation.

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616 Preventing Biofilms on BACTIGON® Coated Camstent Urinary Catheters in Patients Undergoing Elective Colorectal Surgery: A Single Centre Pilot Study

British Journal of Surgery ◽

10.1093/bjs/znab134.015 ◽

2021 ◽

Vol 108 (Supplement_2) ◽

Author(s):

C Lewis-Lloyd ◽

J Dubern ◽

K Kalenderski ◽

N Halliday ◽

M Alexander ◽

...

Keyword(s):

Environmental Scanning Electron Microscopy ◽

Environmental Scanning ◽

Bacterial Cells ◽

Urinary Catheters ◽

Elective Colorectal Surgery ◽

Mineral Compositions ◽

Microscopy Data ◽

Tract Infections ◽

Biofilm Development ◽

Hospital Acquired

Abstract Introduction Catheter associated urinary tract infections account for 40% of hospital acquired infections. They are associated with biofilms consisting of bacterial cells enmeshed in a self-generated extracellular matrix adhering to catheter surfaces. We have developed a novel polymer family that, coated onto urinary catheters, creates a “non-stick” surface preventing biofilm development. Method Prospective cohort of elective colorectal patients recruited pre-operatively, received a standard silicone (SS) or Camstent (BACTIGON®) coated urinary catheter. After removal, catheters were cut longitudinally into 3 segments. Biomass and biomineralisation were analysed using confocal fluorescence microscopy. Data were normalised by square rooting the catheter indwelling duration. Environmental scanning electron microscopy and energy dispersive x-ray spectroscopy was performed. Results Of 40 patients, 20 each received a SS or coated catheter. Between SS and coated catheters, average indwelling duration was similar and biofilm biomass was 32.068µg/cm2 (95%CI ±21.950) vs. 1.948µg/cm2 (95%CI ±2.595) (P = 0.0111). Confocal microscopy suggested a 93.93% reduction in biofilm biomass on coated catheters. Mineral compositions were different with biofilm and struvite/apatite on SS and calcium oxalate, endogenously derived, on coated catheters. Conclusions Inert BACTIGON® coated catheters appear superior at preventing biofilm formation than SS catheters. Clinical trials are needed to determine the clinical and health economic benefit of this intervention.

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