Noninvasive Prognosis of Postmyocardial Infarction Using Urinary miRNA Ultratrace Detection Based on Single-Target DNA-Functionalized AuNPs

2022 ◽  
Author(s):  
Qianlong Wang ◽  
Lancong Liu ◽  
Xiaoyi Chen ◽  
Tiantian Wang ◽  
Hua Zhou ◽  
...  
Keyword(s):  
Postmyocardial Infarction ◽  
Single Target ◽  
Target Dna ◽  
Ultratrace Detection ◽  
Functionalized Aunps

scholarly journals Computational design of probes to detect bacterial genomes by multivalent binding

2020 ◽  
Vol 117 (16) ◽  
pp. 8719-8726 ◽  
Author(s):  
Tine Curk ◽  
Chris A. Brackley ◽  
James D. Farrell ◽  
Zhongyang Xing ◽  
Darshana Joshi ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Bacterial Infections ◽  
Bacterial Genome ◽  
Computational Design ◽  
Diagnostic Methods ◽  
Detection Sensitivity ◽  
Target Sequence ◽  
Inappropriate Use ◽  
Single Target ◽  
Target Dna

Rapid methods for diagnosis of bacterial infections are urgently needed to reduce inappropriate use of antibiotics, which contributes to antimicrobial resistance. In many rapid diagnostic methods, DNA oligonucleotide probes, attached to a surface, bind to specific nucleotide sequences in the DNA of a target pathogen. Typically, each probe binds to a single target sequence; i.e., target–probe binding is monovalent. Here we show using computer simulations that the detection sensitivity and specificity can be improved by designing probes that bind multivalently to the entire length of the pathogen genomic DNA, such that a given probe binds to multiple sites along the target DNA. Our results suggest that multivalent targeting of long pieces of genomic DNA can allow highly sensitive and selective binding of the target DNA, even if competing DNA in the sample also contains binding sites for the same probe sequences. Our results are robust to mild fragmentation of the bacterial genome. Our conclusions may also be relevant for DNA detection in other fields, such as disease diagnostics more broadly, environmental management, and food safety.

Investigation of ultra-thin films of YBa2Cu3O7−δ grown on MgO

1990 ◽  
Vol 48 (4) ◽  
pp. 6-7
Author(s):  
S.K. Streiffer ◽  
C.B. Eom ◽  
J.C. Bravman ◽  
T.H. Geballet
Keyword(s):  
Thin Films ◽  
Room Temperature ◽  
Deposition Process ◽  
Nucleation And Growth ◽  
Multilayer Structures ◽  
Rf Power ◽  
Oxygen Loss ◽  
Transmission Electron ◽  
Single Target ◽  
Mtorr Argon

The study of very thin (<15 nm) YBa2Cu3O7−δ (YBCO) films is necessary both for investigating the nucleation and growth of films of this material and for achieving a better understanding of multilayer structures incorporating such thin YBCO regions. We have used transmission electron microscopy to examine ultra-thin films grown on MgO substrates by single-target, off-axis magnetron sputtering; details of the deposition process have been reported elsewhere. Briefly, polished MgO substrates were attached to a block placed at 90° to the sputtering target and heated to 650 °C. The sputtering was performed in 10 mtorr oxygen and 40 mtorr argon with an rf power of 125 watts. After deposition, the chamber was vented to 500 torr oxygen and allowed to cool to room temperature. Because of YBCO’s susceptibility to environmental degradation and oxygen loss, the technique of Xi, et al. was followed and a protective overlayer of amorphous YBCO was deposited on the just-grown films.

Single-Block, Single-Target, Implicit Association Test

2011 ◽  
Author(s):  
Axel Zinkernagel ◽  
Wilhelm Hofmann ◽  
Friederike X. R. Dislich ◽  
Tobias Gschwendner ◽  
Manfred Schmitt
Keyword(s):  
Implicit Association Test ◽  
Association Test ◽  
Implicit Association ◽  
Single Target ◽  
Single Block

Assessment of the implicit attitude of Japanese people toward blacks and Little Black Sambo

2018 ◽  
Author(s):  
Kazuo Mori
Keyword(s):  
High School Students ◽  
Junior High School ◽  
Implicit Attitudes ◽  
Picture Book ◽  
Implicit Attitude ◽  
Japanese People ◽  
School Students ◽  
Negative Attitudes ◽  
Single Target ◽  
Significant Difference

We examined whether Japanese people, 47 junior high school students, 49 undergraduates, and 52 older adults, possessed negative attitudes against blacks and the picture book Little Black Sambo. We assessed the implicit attitude toward the target word pairs, “black/white” and “Sambo/Heidi,” by utilizing a paper-based Implicit Association Test and found that both black and Sambo were associated more negatively than white and Heidi. However, the implicit attitudes assessed with a single-target IAT showed that 67 Japanese students showed positive implicit scores for blacks but with smaller valences. A post hoc analysis revealed that the reading experience of Little Black Sambo did not show a significant difference between the implicit attitudes of those who had and had not read the book.

Voice Conversion Across Arbitrary Speakers Based on a Single Target-Speaker Utterance

2018 ◽  
Author(s):  
Songxiang Liu ◽  
Jinghua Zhong ◽  
Lifa Sun ◽  
Xixin Wu ◽  
Xunying Liu ◽  
...  
Keyword(s):  
Voice Conversion ◽  
Single Target ◽  
Target Speaker
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