A Photothermal Nanoplatform with Sugar-Triggered Cleaning Ability for High-Efficiency Intracellular Delivery

2022 ◽  
Author(s):  
Kunyan Lu ◽  
Yangcui Qu ◽  
Yuancheng Lin ◽  
Luohuizi Li ◽  
Yan Wu ◽  
...  
Keyword(s):  
High Efficiency ◽  
Intracellular Delivery

scholarly journals A boronic acid–rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing

2019 ◽  
Vol 5 (6) ◽  
pp. eaaw8922 ◽  
Author(s):  
Chongyi Liu ◽  
Tao Wan ◽  
Hui Wang ◽  
Song Zhang ◽  
Yuan Ping ◽  
...  
Keyword(s):  
Boronic Acid ◽  
Protein Delivery ◽  
High Efficiency ◽  
Intracellular Delivery ◽  
Cytosolic Protein ◽  
Native Proteins ◽  
Cytosolic Delivery ◽  
Cas9 Protein ◽  
Cargo Proteins ◽  
Positively Charged

Cytosolic protein delivery is of central importance for the development of protein-based biotechnologies and therapeutics; however, efficient intracellular delivery of native proteins remains a challenge. Here, we reported a boronic acid–rich dendrimer with unprecedented efficiency for cytosolic delivery of native proteins. The dendrimer could bind with both negatively and positively charged proteins and efficiently delivered 13 cargo proteins into the cytosol of living cells. All the delivered proteins kept their bioactivities after cytosolic delivery. The dendrimer ensures efficient intracellular delivery of Cas9 protein into various cell lines and showed high efficiency in CRISPR-Cas9 genome editing. The rationally designed boronic acid–rich dendrimer permits the development of an efficient platform with high generality for the delivery of native proteins.

Poloxamer/sodium cholate co-formulation for micellar encapsulation of doxorubicin with high efficiency for intracellular delivery: An in-vitro bioavailability study

2020 ◽  
Vol 579 ◽  
pp. 551-561 ◽  
Author(s):  
Elisamaria Tasca ◽  
Patrizia Andreozzi ◽  
Alessandra Del Giudice ◽  
Luciano Galantini ◽  
Karin Schillén ◽  
...  
Keyword(s):  
High Efficiency ◽  
Intracellular Delivery ◽  
Sodium Cholate ◽  
Bioavailability Study

Nanoscale Metal-organic Frameworks for Intracellular Delivery of CRISPR/Cas9 Genome Editing Machinery

2021 ◽  
Author(s):  
Qizhen Zheng ◽  
Wenting Li ◽  
Lanqun Mao ◽  
Ming Wang
Keyword(s):  
Genome Editing ◽  
Dna Sequence ◽  
High Efficiency ◽  
Genetic Disorders ◽  
Metal Organic Frameworks ◽  
Intracellular Delivery ◽  
Metal Organic

The discovery of CRISPR/Cas9 genome-editing technology enables the precise manipulation of DNA sequence of mammalians for treating genetic disorders. Despite its high efficiency for genome editing, the introduction of CRISPR/Cas9...

Two-in-One Platform for High-Efficiency Intracellular Delivery and Cell Harvest: When a Photothermal Agent Meets a Thermoresponsive Polymer

2019 ◽  
Vol 11 (13) ◽  
pp. 12357-12366 ◽  
Author(s):  
Jingxian Wu ◽  
Yanjun Zheng ◽  
Shuaibing Jiang ◽  
Yangcui Qu ◽  
Ting Wei ◽  
...  
Keyword(s):  
High Efficiency ◽  
Intracellular Delivery ◽  
Thermoresponsive Polymer ◽  
Cell Harvest

A Universal Platform for High‐Efficiency “Engineering” Living Cells: Integration of Cell Capture, Intracellular Delivery of Biomolecules, and Cell Harvesting Functions

2019 ◽  
Vol 30 (3) ◽  
pp. 1906362 ◽  
Author(s):  
Yangcui Qu ◽  
Yanjun Zheng ◽  
Liyin Yu ◽  
Yang Zhou ◽  
Yaran Wang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Intracellular Delivery ◽  
Living Cells ◽  
Cell Capture ◽  
Cell Harvesting

Shiga-like toxin-based high-efficiency and receptor-specific intracellular delivery system for a protein

2015 ◽  
Vol 464 (4) ◽  
pp. 1282-1289 ◽  
Author(s):  
Jeong-Hyun Ryou ◽  
Yoo-Kyoung Sohn ◽  
Da-Eun Hwang ◽  
Hak-Sung Kim
Keyword(s):  
Delivery System ◽  
High Efficiency ◽  
Intracellular Delivery

scholarly journals High-throughput and dosage-controlled intracellular delivery of large cargos by an acoustic-electric micro-vortices platform

2021 ◽  
Author(s):  
Mohammad Aghaamoo ◽  
Yu-Hsi Chen ◽  
Xuan Li ◽  
Neha Garg ◽  
Ruoyu Jiang ◽  
...  
Keyword(s):  
High Efficiency ◽  
Gene Knockout ◽  
Intracellular Delivery ◽  
Cell Types ◽  
Specific Cell ◽  
Single Chip ◽  
Dna Encoding ◽  
Suspension Cells ◽  
Wide Range ◽  
Large Plasmids

ABSTRACTIntracellular delivery of cargos for cell engineering plays a pivotal role in transforming medicine and biomedical discoveries. Recent advances in microfluidics and nanotechnology have opened up new avenues for efficient, safe, and controllable intracellular delivery, as they improve precision down to the single-cell level. Based on this capability, several promising micro- and nanotechnology approaches outperform viral and conventional non-viral techniques in offering dosage-controlled delivery and/or intracellular delivery of large cargos. However, to achieve this level of precision and effectiveness, they are either low in throughput, limited to specific cell types (e.g., adherent vs. suspension cells), or complicated to operate with. To address these challenges, here we introduce a versatile and simple-to-use intracellular delivery microfluidic platform, termed Acoustic-Electric Shear Orbiting Poration (AESOP). Hundreds of acoustic microstreaming vortices form the production line of the AESOP platform, wherein hundreds of thousands of cells are trapped, permeabilized, and mixed with exogenous cargos. Using AESOP, we show intracellular delivery of a wide range of molecules (from <1 kDa to 2 MDa) with high efficiency, cell viability, and dosage-controlled capability into both suspension and adherent cells and demonstrate throughput at 1 million cells/min per single chip. In addition, we demonstrate AESOP for two gene editing applications that require delivery of large plasmids: i) eGFP plasmid (6.1 kbp) transfection, and ii) CRISPR-Cas9-mediated gene knockout using a 9.3 kbp plasmid DNA encoding Cas9 protein and sgRNA. Compared to alternative platforms, AESOP not only offers dosage-controlled intracellular delivery of large plasmids (>6kbp) with viabilities over 80% and comparable delivery efficiencies, but also is an order of magnitude higher in throughput, compatible with both adherent and suspension cell lines, and simple to operate.

Microcalorimeters for X-Ray Spectroscopy

1988 ◽  
Vol 102 ◽  
pp. 41
Author(s):  
E. Silver ◽  
C. Hailey ◽  
S. Labov ◽  
N. Madden ◽  
D. Landis ◽  
...  
Keyword(s):  
High Resolution ◽  
High Efficiency ◽  
Thermal Response ◽  
Low Noise ◽  
High Resolution Spectroscopy ◽  
Superconducting Transition ◽  
Sapphire Substrates ◽  
Laboratory Plasmas ◽  
Adiabatic Demagnetization ◽  
Theoretical Predictions

The merits of microcalorimetry below 1°K for high resolution spectroscopy has become widely recognized on theoretical grounds. By combining the high efficiency, broadband spectral sensitivity of traditional photoelectric detectors with the high resolution capabilities characteristic of dispersive spectrometers, the microcalorimeter could potentially revolutionize spectroscopic measurements of astrophysical and laboratory plasmas. In actuality, however, the performance of prototype instruments has fallen short of theoretical predictions and practical detectors are still unavailable for use as laboratory and space-based instruments. These issues are currently being addressed by the new collaborative initiative between LLNL, LBL, U.C.I., U.C.B., and U.C.D.. Microcalorimeters of various types are being developed and tested at temperatures of 1.4, 0.3, and 0.1°K. These include monolithic devices made from NTD Germanium and composite configurations using sapphire substrates with temperature sensors fabricated from NTD Germanium, evaporative films of Germanium-Gold alloy, or material with superconducting transition edges. A new approache to low noise pulse counting electronics has been developed that allows the ultimate speed of the device to be determined solely by the detector thermal response and geometry. Our laboratory studies of the thermal and resistive properties of these and other candidate materials should enable us to characterize the pulse shape and subsequently predict the ultimate performance. We are building a compact adiabatic demagnetization refrigerator for conveniently reaching 0.1°K in the laboratory and for use in future satellite-borne missions. A description of this instrument together with results from our most recent experiments will be presented.

Imaging and diffraction modes in Scanning Transmission Electron Microscopy

1986 ◽  
Vol 44 ◽  
pp. 684-687
Author(s):  
J. M. Cowley ◽  
R. Glaisher ◽  
J. A. Lin ◽  
H.-J. Ou
Keyword(s):  
Scanning Transmission Electron Microscopy ◽  
High Efficiency ◽  
Fiber Optic ◽  
Image Intensifier ◽  
Detector System ◽  
Detector Plane ◽  
Secondary Electrons ◽  
Transmission Electron ◽  
Scanning Transmission ◽  
Special Detector

Some of the most important applications of STEM depend on the variety of imaging and diffraction made possible by the versatility of the detector system and the serial nature, of the image acquisition. A special detector system, previously described, has been added to our STEM instrument to allow us to take full advantage of this versatility. In this, the diffraction pattern in the detector plane may be formed on either of two phosphor screens, one with P47 (very fast) phosphor and the other with P20 (high efficiency) phosphor. The light from the phosphor is conveyed through a fiber-optic rod to an image intensifier and TV system and may be photographed, recorded on videotape, or stored digitally on a frame store. The P47 screen has a hole through it to allow electrons to enter a Gatan EELS spectrometer. Recently a modified SEM detector has been added so that high resolution (10Å) imaging with secondary electrons may be used in conjunction with other modes.

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