Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification

2017 ◽  
Vol 9 (18) ◽  
pp. 15245-15253 ◽  
Author(s):  
Fan Li ◽  
Xiaoguo Liu ◽  
Bin Zhao ◽  
Juan Yan ◽  
Qian Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Real Time Monitoring ◽  
Isothermal Nucleic Acid Amplification

scholarly journals A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

Sensors ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 23418-23430 ◽  
Author(s):  
Pascal Craw ◽  
Ruth Mackay ◽  
Angel Naveenathayalan ◽  
Chris Hudson ◽  
Manoharanehru Branavan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Low Cost ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

scholarly journals An IoT-enabled paper sensor platform for real-time analysis of isothermal nucleic acid amplification tests

2020 ◽  
Vol 169 ◽  
pp. 112651 ◽  
Author(s):  
Mingdian Liu ◽  
Yuxin Zhao ◽  
Hosein Monshat ◽  
Zheyuan Tang ◽  
Zuowei Wu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Time Analysis ◽  
Nucleic Acid Amplification Tests ◽  
Isothermal Nucleic Acid Amplification ◽  
Real Time Analysis ◽  
Sensor Platform ◽  
Paper Sensor

scholarly journals Real-time isothermal nucleic acid amplification detection in resource-limited settings: a description of an open-source miniature fluorimeter

2021 ◽  
Vol 9 ◽  
pp. S6
Author(s):  
Jackson Coole ◽  
Alex Kortum ◽  
Yubo Tang ◽  
Imran Vohra ◽  
Mary Natoli ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Open Source ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Resource Limited Settings ◽  
Isothermal Nucleic Acid Amplification ◽  
Resource Limited

An integrated portable hand-held analyser for real-time isothermal nucleic acid amplification

2007 ◽  
Vol 598 (2) ◽  
pp. 286-294 ◽  
Author(s):  
Matthew C. Smith ◽  
George Steimle ◽  
Stan Ivanov ◽  
Mark Holly ◽  
David P. Fries
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals Rapid Diagnosis of Tick-Borne Illnesses by Use of One-Step Isothermal Nucleic Acid Amplification and Bio-Optical Sensor Detection

2018 ◽  
Vol 64 (3) ◽  
pp. 556-565 ◽  
Author(s):  
Ji Yeun Kim ◽  
Bonhan Koo ◽  
Choong Eun Jin ◽  
Min Chul Kim ◽  
Yong Pil Chong ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Scrub Typhus ◽  
Nucleic Acid Amplification ◽  
Blood Samples ◽  
Clinical Sensitivity ◽  
Isothermal Nucleic Acid Amplification ◽  
One Step ◽  
Sensor Detection

Abstract BACKGROUND Scrub typhus and severe fever with thrombocytopenia syndrome (SFTS) are the most common tick-borne illnesses in South Korea. Early differentiation of SFTS from scrub typhus in emergency departments is essential but difficult because of their overlapping epidemiology, shared risk factors, and similar clinical manifestations. METHODS We compared the diagnostic performance of one-step isothermal nucleic acid amplification with bio-optical sensor detection (iNAD) under isothermal conditions, which is rapid (20–30 min), with that of real-time PCR, in patients with a confirmed tick-borne illness. Fifteen patients with confirmed SFTS who provided a total of 15 initial blood samples and 5 follow-up blood samples, and 21 patients with confirmed scrub typhus, were evaluated. RESULTS The clinical sensitivity of iNAD (100%; 95% CI, 83–100) for SFTS was significantly higher than that of real-time PCR (75%; 95% CI, 51–91; P = 0.047), while its clinical specificity (86%; 95% CI, 65–97) was similar to that of real-time PCR (95%; 95% CI, 77–99; P = 0.61). The clinical sensitivity of iNAD for scrub typhus (100%; 95% CI, 81–100) was significantly higher than that of real-time PCR for scrub typhus (67%; 95% CI, 43–85; P = 0.009), while its clinical specificity (90%; 95% CI, 67–98) was similar to that of real-time PCR (95%; 95% CI, 73–100; P > 0.99). CONCLUSIONS iNAD is a valuable, rapid method of detecting SFTS virus and Orientia tsutsugamushi with high clinical sensitivity and specificity.

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