scholarly journals Allele-specific recombinase polymerase amplification for real-time detection of sickle cell anaemia in low-resource settings: evaluation of an isothermal nucleic acid amplification test to detect the βS globin point mutation in paediatric patients

2021 ◽  
Vol 9 ◽  
pp. S13
Author(s):  
Mary Natoli ◽  
Megan Chang ◽  
Kathryn Kundrod ◽  
Jackson Coole ◽  
Gladstone Airewele ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Mutation ◽  
Sickle Cell ◽  
Sickle Cell Anaemia ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Low Resource Settings ◽  
Isothermal Nucleic Acid Amplification ◽  
Allele Specific

scholarly journals A Simple, Low-Cost Platform for Real-Time Isothermal Nucleic Acid Amplification

Sensors ◽  
2015 ◽  
Vol 15 (9) ◽  
pp. 23418-23430 ◽  
Author(s):  
Pascal Craw ◽  
Ruth Mackay ◽  
Angel Naveenathayalan ◽  
Chris Hudson ◽  
Manoharanehru Branavan ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Low Cost ◽  
Nucleic Acid Amplification ◽  
Isothermal Nucleic Acid Amplification

scholarly journals 1402Multicenter Clinical Evaluation of the Novel Alere i Strep Isothermal Nucleic Acid Amplification Test

2014 ◽  
Vol 1 (suppl_1) ◽  
pp. S369-S369
Author(s):  
Daniel Cohen ◽  
Michael Russo ◽  
Keith Vrbicky ◽  
Preeti Jaggi ◽  
William Gluckman ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Clinical Evaluation ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
The Novel ◽  
Isothermal Nucleic Acid Amplification

Graphene Nanoprobes for Real-Time Monitoring of Isothermal Nucleic Acid Amplification

2017 ◽  
Vol 9 (18) ◽  
pp. 15245-15253 ◽  
Author(s):  
Fan Li ◽  
Xiaoguo Liu ◽  
Bin Zhao ◽  
Juan Yan ◽  
Qian Li ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Nucleic Acid Amplification ◽  
Real Time Monitoring ◽  
Isothermal Nucleic Acid Amplification

scholarly journals A Robust, Low-Cost Instrument for Real-time Colorimetric Isothermal Nucleic Acid Amplification

2021 ◽  
Author(s):  
Frank Myers ◽  
Brian Moffatt ◽  
Ragheb El Khaja ◽  
Titash Chatterjee ◽  
Gurmeet Marwaha ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Point Of Care ◽  
Low Cost ◽  
Lamp Assay ◽  
Nucleic Acid Amplification ◽  
Diagnostic Assays ◽  
Isothermal Nucleic Acid Amplification ◽  
Point Of Care Diagnostics ◽  
Time Readout

The COVID-19 pandemic has highlighted the need for broader access to molecular diagnostics. Colorimetric isothermal nucleic acid amplification assays enable simplified instrumentation over more conventional PCR diagnostic assays and, as such, represent a promising approach for addressing this need. In particular, colorimetric LAMP (loop-mediated isothermal amplification) has received a great deal of interest recently. However, there do not currently exist robust instruments for performing these kinds of assays in high throughput with real-time readout of amplification signals. To address this need, we developed LARI, the LAMP Assay Reader Instrument. We have deployed over 50 LARIs for routine use in R&D and production environments, with over 12,000 assays run to date. In this paper, we present the design and construction of LARI along with thermal, optical, and assay performance characteristics. LARI can be produced for under $1500 and has broad applications in R&D, point-of-care diagnostics, and global health.

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