Assembly Pathway Selection of Designer Self-Assembling Peptide and Fabrication of Hierarchical Scaffolds for Neural Regeneration

2018 ◽  
Vol 10 (31) ◽  
pp. 26128-26141 ◽  
Author(s):  
Yuyuan Zhao ◽  
Rong Zhu ◽  
Xiyong Song ◽  
Zheng Ma ◽  
Shengfeng Chen ◽  
...  
Keyword(s):  
Neural Regeneration ◽  
Self Assembling ◽  
Assembly Pathway ◽  
Selection Of

Genetic Exchange Leading to Self-Assembling RNA Species upon Encapsulation in Artificial Protocells

2007 ◽  
Vol 13 (3) ◽  
pp. 279-289 ◽  
Author(s):  
Sergio-Francis M. Zenisek ◽  
Eric J. Hayden ◽  
Niles Lehman
Keyword(s):  
Self Assembly ◽  
Divalent Cations ◽  
Direct Interaction ◽  
Living Systems ◽  
Oil Emulsion ◽  
Rna Molecules ◽  
Self Assembling ◽  
Assembly Pathway ◽  
Water In Oil Emulsion ◽  
Recombination Reactions

The encapsulation of information-bearing macromolecules inside protocells is a critical step in scenarios for the origins of life on the Earth as well as for the construction of artificial living systems. For these protocells to emulate life, they must be able to transmit genetic information to other cells. We have used a water-in-oil emulsion system to simulate the compartmentalization of catalytic RNA molecules. By exploiting RNA-directed recombination reactions previously developed in our laboratory, including a ribozyme self-assembly pathway, we demonstrate that it is possible for information to be exchanged among protocells. This can happen either indirectly by the passage of divalent cations through the inter-protocellular medium (oil), or by the direct interaction of two or more protocells that allows RNA molecules to be exchanged. The degree of agitation affects the ability of such exchange. The consequences of these results include the implications that prototypical living systems can transmit information among compartments, and that the environment can regulate the extent of this crosstalk.

Defects in cytochrome oxidase assembly in humans: lessons from yeastThis paper is one of a selection of papers published in this Special Issue, entitled CSBMCB — Membrane Proteins in Health and Disease.

2006 ◽  
Vol 84 (6) ◽  
pp. 859-869 ◽  
Author(s):  
Jennifer M. Zee ◽  
D. Moira Glerum
Keyword(s):  
Molecular Mechanisms ◽  
Inner Mitochondrial Membrane ◽  
Yeast Saccharomyces Cerevisiae ◽  
Assembly Pathway ◽  
Complex Process ◽  
Health And Disease ◽  
Molecular Bases ◽  
Near Future ◽  
Insight Into ◽  
Selection Of

The biogenesis of the inner mitochondrial membrane enzyme cytochrome c oxidase (COX) is a complex process that requires the actions of ancillary proteins, collectively called assembly factors. Studies with the yeast Saccharomyces cerevisiae have provided considerable insight into the COX assembly pathway and have proven to be a fruitful model for understanding the molecular bases for inherited COX deficiencies in humans. In this review, we focus on critical steps in the COX assembly pathway. These processes are conserved from yeast to humans and are known to be involved in the etiology of human COX deficiencies. The contributions from our studies in yeast suggest that this organism remains an excellent model system for delineating the molecular mechanisms underlying COX assembly defects in humans. Current progress suggests that a complete picture of COX assembly will be achieved in the near future.

Self-Assembling Peptides Mediate Neural Regeneration

2015 ◽  
pp. 229-236 ◽  
Author(s):  
Jiasong Guo ◽  
Kwok-Fai So ◽  
Wutian Wu
Keyword(s):  
Neural Regeneration ◽  
Self Assembling

α-Hemolysin: A Self-Assembling Protein Pore With Potential Applications In The Synthesis of New Materials

1991 ◽  
Vol 255 ◽  
Author(s):  
Hagan Bayley ◽  
Musti Krishnasastry ◽  
Barbara Walker ◽  
John Kasianowicz
Keyword(s):  
Genetic Manipulation ◽  
Internal Diameter ◽  
New Materials ◽  
Membrane Pores ◽  
Self Assembling ◽  
Assembly Mechanism ◽  
Potential Applications ◽  
Membrane Bound ◽  
Insight Into ◽  
Selection Of

AbstractA selection of nanoscale membrane pores is being constructed by genetic manipulation of α-hemolysin (αHL), a 33.2 kDa polypeptide secreted by the bacterium Staphylococcus aureus, which can self-assemble into hexameric cylindrical channels -1 to 2 nm In Internal diameter. Ultimately, the new pores will be used to confer novel permeability properties upon materials such as thin films utilizing, for example, monolayer sheets of the hexamer. Recombinant αHL (r-αHL) has now been obtained in multimilligram amounts and purified to homogeneity after overexpression of the αHL gene in Escherichia coli. The properties of r-αHL are closely similar to those of αHL purified from S. aureus. Recent deletion mutagenesis experiments have given us new insight into the assembly mechanism of the pore. Three intermediates have been identified: a membrane-bound monomer; an oligomeric pore precursor; and the hexameric pore itself. Currently, point mutogenesis combined with chemical modification is being used to produce new pores of different internal diameter, with selectivity for the passage of molecules and Ions, and with gating properties (the ability to open and close in response to a physical stimulus, e.g. an electric field or light).

scholarly journals Allosteric pathway selection in templated assembly

2019 ◽  
Vol 5 (10) ◽  
pp. eaaw3353 ◽  
Author(s):  
Martijn van Galen ◽  
Ruben Higler ◽  
Joris Sprakel
Keyword(s):  
Building Blocks ◽  
Supramolecular Interactions ◽  
High Fidelity ◽  
Self Assembling ◽  
Molecular Building Blocks ◽  
Large Numbers ◽  
Solution Aggregation ◽  
Allosteric Pathway ◽  
Insight Into ◽  
Selection Of

Assembling large numbers of molecular building blocks into functional nanostructures is no trivial task. It relies on guiding building blocks through complex energy landscapes shaped by synergistic and antagonistic supramolecular interactions. In nature, the use of molecular templates is a potent strategy to navigate the process to the desired structure with high fidelity. Yet, nature’s templating strategy remains to be fully exploited in man-made nanomaterials. Designing effective template-guided self-assembling systems can only be realized through precise insight into how the chemical design of building blocks and the resulting balance of repulsive and attractive forces give rise to pathway selection and suppression of trapped states. We develop a minimal model to unravel the kinetic pathways and pathway selection of the templated assembly of molecular building blocks on a template. We show how allosteric activation of the associative interactions can suppress undesired solution-aggregation pathways and gives rise to a true template-assembly path.

Complete Selection of a Self-Assembling Homo- or Hetero-Cavitand Cage via Metal Coordination Based on Ligand Tuning

2004 ◽  
Vol 126 (43) ◽  
pp. 13896-13897 ◽  
Author(s):  
Kenji Kobayashi ◽  
Yoshifumi Yamada ◽  
Masamichi Yamanaka ◽  
Yoshihisa Sei ◽  
Kentaro Yamaguchi
Keyword(s):  
Metal Coordination ◽  
Self Assembling ◽  
Selection Of

scholarly journals Delivery of mRNA Vaccine against SARS-CoV-2 Using a Polyglucin:Spermidine Conjugate

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 76
Author(s):  
Larisa I. Karpenko ◽  
Andrey P. Rudometov ◽  
Sergei V. Sharabrin ◽  
Dmitry N. Shcherbakov ◽  
Mariya B. Borgoyakova ◽  
...  
Keyword(s):  
Zero Point ◽  
The Self ◽  
Charge Ratio ◽  
Receptor Binding Domain ◽  
Specific Antibodies ◽  
Electrophoretic Light Scattering ◽  
Self Assembling ◽  
Monodisperse Suspension ◽  
Positively Charged ◽  
Selection Of

One of the key stages in the development of mRNA vaccines is their delivery. Along with liposome, other materials are being developed for mRNA delivery that can ensure both the safety and effectiveness of the vaccine, and also facilitate its storage and transportation. In this study, we investigated the polyglucin:spermidine conjugate as a carrier of an mRNA-RBD vaccine encoding the receptor binding domain (RBD) of the SARS-CoV-2 spike protein. The conditions for the self-assembling of mRNA-PGS complexes were optimized, including the selection of the mRNA:PGS charge ratios. Using dynamic and electrophoretic light scattering it was shown that the most monodisperse suspension of nanoparticles was formed at the mRNA:PGS charge ratio equal to 1:5. The average hydrodynamic particles diameter was determined, and it was confirmed by electron microscopy. The evaluation of the zeta potential of the investigated complexes showed that the particles surface charge was close to the zero point. This may indicate that the positively charged PGS conjugate has completely packed the negatively charged mRNA molecules. It has been shown that the packaging of mRNA-RBD into the PGS envelope leads to increased production of specific antibodies with virus-neutralizing activity in immunized BALB/c mice. Our results showed that the proposed polycationic polyglucin:spermidine conjugate can be considered a promising and safe means to the delivery of mRNA vaccines, in particular mRNA vaccines against SARS-CoV-2.

Selection of the Appropriate Parameter to Measure Neural Regeneration

1989 ◽  
Vol 23 (3) ◽  
pp. 197-202 ◽  
Author(s):  
A Lee Dellon ◽  
Susan E. Mackinnon
Keyword(s):  
Neural Regeneration ◽  
Selection Of

PLGA nanofibers blended with designer self-assembling peptides for peripheral neural regeneration

2016 ◽  
Vol 62 ◽  
pp. 329-337 ◽  
Author(s):  
Manasa Nune ◽  
Uma Maheswari Krishnan ◽  
Swaminathan Sethuraman
Keyword(s):  
Neural Regeneration ◽  
Self Assembling

scholarly journals Effect of heterocyclic capping groups on the self-assembly of a dipeptide hydrogel

Soft Matter ◽  
2016 ◽  
Vol 12 (10) ◽  
pp. 2700-2707 ◽  
Author(s):  
Adam D. Martin ◽  
Jonathan P. Wojciechowski ◽  
Holly Warren ◽  
Marc in het Panhuis ◽  
Pall Thordarson
Keyword(s):  
Mechanical Strength ◽  
Network Structure ◽  
Self Assembly ◽  
The Self ◽  
Assembly Pathway ◽  
Selection Of

In this work it is shown that the selection of heterocyclic capping group for a dipeptide hydrogel affects the self-assembly pathway taken, resulting in differences in mechanical strength, network structure and thixotropic properties.

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