Keratin-Associated Protein Nanoparticles as Hemostatic Agents

Patients undergoing re-operative cardiac surgical procedures present a great challenge with regard to obtaining hemostasis in the surgical field. Adhesions are ever-present and these patients are often on oral anti-coagulants and platelet inhibitors. As part of a well-planned surgical intervention, a systematic approach to hemostasis should be employed to decrease blood transfusion requirement and improve patient outcomes. Topical hemostatic agents can be a great help to the surgeon in achieving surgical field hemostasis and are increasingly being employed. Our approach, to these difficult patients, includes the systematic and planned use of AristaAH, which is a novel hemostatic agent whose use has proven safe and efficacious in our patient population.

Download Full-text

Ca 2+ -induced soy protein nanoparticles as pickering stabilizers: Fabrication and characterization

Food Hydrocolloids ◽

10.1016/j.foodhyd.2016.11.011 ◽

2017 ◽

Vol 65 ◽

pp. 175-186 ◽

Cited By ~ 38

Author(s):

Fu Liu ◽

Shi-Yi Ou ◽

Chuan-He Tang

Keyword(s):

Soy Protein ◽

Protein Nanoparticles ◽

Fabrication And Characterization

Download Full-text

Effect of heat-treated tea water-insoluble protein nanoparticles on the characteristics of pickering emulsions

LWT ◽

10.1016/j.lwt.2021.111999 ◽

2021 ◽

pp. 111999

Author(s):

Zhongyang Ren ◽

Zhongzheng Chen ◽

Yuanyuan Zhang ◽

Xiaorong Lin ◽

Zhanming Li ◽

...

Keyword(s):

Pickering Emulsions ◽

Insoluble Protein ◽

Heat Treated ◽

Protein Nanoparticles

Download Full-text

Evaluation and comparison of structurally different cellulose-based hemostatic agents in a rat kidney model

Cellulose ◽

10.1007/s10570-021-04104-1 ◽

2021 ◽

Author(s):

Alice Paprskářová ◽

Pavel Suchý ◽

Marta Chalupová ◽

Lenka Michlovská ◽

Jarmila Klusáková ◽

...

Keyword(s):

Rat Kidney ◽

Hemostatic Agents

Download Full-text

Single-component multilayered self-assembling nanoparticles presenting rationally designed glycoprotein trimers as Ebola virus vaccines

Nature Communications ◽

10.1038/s41467-021-22867-w ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Linling He ◽

Anshul Chaudhary ◽

Xiaohe Lin ◽

Cindy Sou ◽

Tanwee Alkutkar ◽

...

Keyword(s):

Neutralizing Antibodies ◽

Ebola Virus ◽

Heptad Repeat ◽

T Cell Epitopes ◽

Vaccine Strategy ◽

Cell Responses ◽

Self Assembling ◽

Protein Nanoparticles ◽

Main Target ◽

Repertoire Sequencing

AbstractEbola virus (EBOV) glycoprotein (GP) can be recognized by neutralizing antibodies (NAbs) and is the main target for vaccine design. Here, we first investigate the contribution of the stalk and heptad repeat 1-C (HR1C) regions to GP metastability. Specific stalk and HR1C modifications in a mucin-deleted form (GPΔmuc) increase trimer yield, whereas alterations of HR1C exert a more complex effect on thermostability. Crystal structures are determined to validate two rationally designed GPΔmuc trimers in their unliganded state. We then display a modified GPΔmuc trimer on reengineered protein nanoparticles that encapsulate a layer of locking domains (LD) and a cluster of helper T-cell epitopes. In mice and rabbits, GP trimers and nanoparticles elicit cross-ebolavirus NAbs, as well as non-NAbs that enhance pseudovirus infection. Repertoire sequencing reveals quantitative profiles of vaccine-induced B-cell responses. This study demonstrates a promising vaccine strategy for filoviruses, such as EBOV, based on GP stabilization and nanoparticle display.

Download Full-text