Geometric Confinement Guides the Expression of Cancer Stem Cell Molecular Markers CD44 via Cell Traction Forces

2020 ◽  
Vol 6 (8) ◽  
pp. 4623-4630
Author(s):  
Sisi Liu ◽  
Xiaoan Wu ◽  
Yang Yu ◽  
Xiongwei Wen ◽  
Zhang Yu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Molecular Markers ◽  
Cancer Stem Cell ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Cell Traction ◽  
Geometric Confinement

Video-microscopic measurement of cell-substratum traction forces generated by locomoting keratocytes

1993 ◽  
Vol 51 ◽  
pp. 188-189
Author(s):  
Tim Oliver ◽  
Michelle Leonard ◽  
Juliet Lee ◽  
Akira Ishihara ◽  
Ken Jacobson
Keyword(s):  
Silicone Rubber ◽  
Molecular Motors ◽  
Video Frame ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Latex Beads ◽  
Cell Traction ◽  
Local Cell ◽  
Microscopic Measurement ◽  
Kinetics Of

We are using video-enhanced light microscopy to investigate the pattern and magnitude of forces that fish keratocytes exert on flexible silicone rubber substrata. Our goal is a clearer understanding of the way molecular motors acting through the cytoskeleton co-ordinate their efforts into locomotion at cell velocities up to 1 μm/sec. Cell traction forces were previously observed as wrinkles(Fig.l) in strong silicone rubber films by Harris.(l) These forces are now measureable by two independant means.In the first of these assays, weakly crosslinked films are made, into which latex beads have been embedded.(Fig.2) These films report local cell-mediated traction forces as bead displacements in the plane of the film(Fig.3), which recover when the applied force is released. Calibrated flexible glass microneedles are then used to reproduce the translation of individual beads. We estimate the force required to distort these films to be 0.5 mdyne/μm of bead movement. Video-frame analysis of bead trajectories is providing data on the relative localisation, dissipation and kinetics of traction forces.

scholarly journals Cell Traction Forces Direct Fibronectin Matrix Assembly

2009 ◽  
Vol 96 (2) ◽  
pp. 729-738 ◽  
Author(s):  
Christopher A. Lemmon ◽  
Christopher S. Chen ◽  
Lewis H. Romer
Keyword(s):  
Matrix Assembly ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Fibronectin Matrix ◽  
Cell Traction

scholarly journals Dissipation of contractile forces: the missing piece in cell mechanics

2017 ◽  
Vol 28 (14) ◽  
pp. 1825-1832 ◽  
Author(s):  
Laetitia Kurzawa ◽  
Benoit Vianay ◽  
Fabrice Senger ◽  
Timothée Vignaud ◽  
Laurent Blanchoin ◽  
...  
Keyword(s):  
Cell Mechanics ◽  
Force Production ◽  
Traction Force ◽  
Structural Rearrangements ◽  
Force Transmission ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Cell Traction ◽  
Contractile Forces ◽  
Fiber Contraction

Mechanical forces are key regulators of cell and tissue physiology. The basic molecular mechanism of fiber contraction by the sliding of actin filament upon myosin leading to conformational change has been known for decades. The regulation of force generation at the level of the cell, however, is still far from elucidated. Indeed, the magnitude of cell traction forces on the underlying extracellular matrix in culture is almost impossible to predict or experimentally control. The considerable variability in measurements of cell-traction forces indicates that they may not be the optimal readout to properly characterize cell contractile state and that a significant part of the contractile energy is not transferred to cell anchorage but instead is involved in actin network dynamics. Here we discuss the experimental, numerical, and biological parameters that may be responsible for the variability in traction force production. We argue that limiting these sources of variability and investigating the dissipation of mechanical work that occurs with structural rearrangements and the disengagement of force transmission is key for further understanding of cell mechanics.

Development of micropost force sensor array with culture experiments for determination of cell traction forces

2007 ◽  
Vol 64 (7) ◽  
pp. 509-518 ◽  
Author(s):  
Bin Li ◽  
Luke Xie ◽  
Zane C. Starr ◽  
Zhaochun Yang ◽  
Jeen-Shang Lin ◽  
...  
Keyword(s):  
Sensor Array ◽  
Force Sensor ◽  
Traction Forces ◽  
Cell Traction Forces ◽  
Cell Traction

scholarly journals Molecular markers of cancer stem cells verified in vivo

2016 ◽  
Vol 62 (3) ◽  
pp. 228-238 ◽  
Author(s):  
Y.S. Kim ◽  
A.M. Kaidina ◽  
J.H. Chiang ◽  
K.N. Yarygin ◽  
A.Yu. Lupatov
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Molecular Markers ◽  
Cancer Stem Cells ◽  
Cancer Stem Cell ◽  
Stem Cell Markers ◽  
Cancer Stem Cell Markers ◽  
Immunodeficient Mice ◽  
Molecular Features

This systematic review aims to analyze molecular markers of cancer stem cells. Only studies that confirmed tumor-initiating capacity of this population by in vivo assay in immunodeficient mice were included. Final sample of papers that fully correspond with initial aim consists of 97 original studies. The results of their analysis reveal that markers commonly used for cancer stem cells deriving were as follows: CD133, СD44, ALDH, CD34, CD24 and EpCAM. The review also contains description of molecular features of some cancer stem cell markers, modern approaches to cancer treatment by targeting this population and brief assessment of cancer stem cell theory development.

scholarly journals Micropost Force Sensor Array (MFSA) for Measuring Cell Traction Forces

2006 ◽  
Vol 3 (4) ◽  
pp. 195-196
Author(s):  
B. Li ◽  
L. Xie ◽  
Z. C. Starr ◽  
Z. Yang ◽  
J. H-C. Wang
Keyword(s):  
Sensor Array ◽  
Force Sensor ◽  
Traction Forces ◽  
Measuring Cell ◽  
Cell Traction Forces ◽  
Cell Traction
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