In Situ Fabrication of Fiber Reinforced Three-Dimensional Hydrogel Tissue Engineering Scaffolds

Scaffolds is three dimensional structure that serves as a framework for bone growth. Natural materials are often used in synthesis of bone tissue engineering scaffolds with respect to compliance with the content of the human body. Among the materials used to make scafffold was hydroxyapatite, alginate and chitosan. Hydroxyapatite powder obtained by mixing phosphoric acid and calcium hydroxide, alginate powders extracted from brown algae and chitosan powder acetylated from crab. The purpose of this study was to examine the functional groups of hydroxyapatite, alginate and chitosan. The method used in this study was laboratory experimental using Fourier Transform Infrared (FTIR) spectroscopy for hydroxyapatite, alginate and chitosan powders. The results indicated the presence of functional groups PO43-, O-H and CO32- in hydroxyapatite. In alginate there were O-H, C=O, COOH and C-O-C functional groups, whereas in chitosan there were O-H, N-H, C=O, C-N, and C-O-C. It was concluded that the third material containing functional groups as found in humans that correspond to the scaffolds material in bone tissue engineering.

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Synthesis and Characterization of Electrospun Nanofibrous Tissue Engineering Scaffolds Generated from in Situ Polymerization of Ionomeric Polyurethane Composites

SSRN Electronic Journal ◽

10.2139/ssrn.3368398 ◽

2019 ◽

Author(s):

Jennifer PY Chan ◽

J. Paul Santerre

Keyword(s):

Tissue Engineering ◽

In Situ Polymerization ◽

Synthesis And Characterization ◽

Tissue Engineering Scaffolds ◽

Polyurethane Composites

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In Situ Tissue Engineering Using Magnetically Guided Three-Dimensional Cell Patterning

Tissue Engineering Part C Methods ◽

10.1089/ten.tec.2011.0525 ◽

2012 ◽

Vol 18 (7) ◽

pp. 496-506 ◽

Cited By ~ 21

Author(s):

Shawn P. Grogan ◽

Chantal Pauli ◽

Peter Chen ◽

Jiang Du ◽

Christine B. Chung ◽

...

Keyword(s):

Tissue Engineering ◽

Three Dimensional ◽

Cell Patterning ◽

Dimensional Cell ◽

In Situ Tissue Engineering

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Three-Dimensional Microfluidic Tissue-Engineering Scaffolds Using a Flexible Biodegradable Polymer

Advanced Materials ◽

10.1002/adma.200500438 ◽

2006 ◽

Vol 18 (2) ◽

pp. 165-169 ◽

Cited By ~ 219

Author(s):

C. J. Bettinger ◽

E. J. Weinberg ◽

K. M. Kulig ◽

J. P. Vacanti ◽

Y. Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Biodegradable Polymer ◽

Three Dimensional ◽

Tissue Engineering Scaffolds

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Mineralized Nanofibers for Bone Tissue Engineering

Biomedical Engineering ◽

10.4018/978-1-5225-3158-6.ch020 ◽

2018 ◽

pp. 461-475 ◽

Cited By ~ 1

Author(s):

Ozan Karaman

Keyword(s):

Tissue Engineering ◽

Bone Tissue Engineering ◽

Bone Regeneration ◽

Bone Tissue ◽

Three Dimensional ◽

Bone Grafts ◽

Tissue Engineering Scaffolds ◽

Promising Alternative ◽

Biomimetic Scaffolds

The limitation of orthopedic fractures and large bone defects treatments has brought the focus on fabricating bone grafts that could enhance ostegenesis and vascularization in-vitro. Developing biomimetic materials such as mineralized nanofibers that can provide three-dimensional templates of the natural bone extracellular-matrix is one of the most promising alternative for bone regeneration. Understanding the interactions between the structure of the scaffolds and cells and therefore the control cellular pathways are critical for developing functional bone grafts. In order to enhance bone regeneration, the engineered scaffold needs to mimic the characteristics of composite bone ECM. This chapter reviews the fabrication of and fabrication techniques for fabricating biomimetic bone tissue engineering scaffolds. In addition, the chapter covers design criteria for developing the scaffolds and examples of enhanced osteogenic differentiation outcomes by fabricating biomimetic scaffolds.

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The Fabrication of Tissue Engineering Scaffolds by Inkjet Printing Technology

Materials Science Forum ◽

10.4028/www.scientific.net/msf.934.129 ◽

2018 ◽

Vol 934 ◽

pp. 129-133 ◽

Cited By ~ 1

Author(s):

Chao Fan Lv ◽

Li Ya Zhu ◽

Jian Ping Shi ◽

Zong An Li ◽

Wen Lai Tang ◽

...

Keyword(s):

Tissue Engineering ◽

Sodium Alginate ◽

Inkjet Printing ◽

Three Dimensional ◽

3D Bioprinting ◽

Tissue Engineering Scaffolds ◽

Printing Technology ◽

3D Printed ◽

Inkjet Printing Technology ◽

Alginate Scaffold

Three-dimensional (3D) printing has been playing an important role in diverse areas in medicine. In order to promote the development of tissue engineering, this study attempts to fabricate tissue engineering scaffolds using the inkjet printing technology. Sodium alginate, exhibiting similar properties to the native human extracellular matrix (ECM), was used as bioink. The jetted fluid of sodium alginate would be gelatinized when printed into the calcium chloride solution. The characteristics of the 3D-printed sodium alginate scaffold were systematically measured and analyzed. The results show that, the pore size, porosity and degradation property of these scaffolds could be well controlled. This study indicates the capability of 3D bioprinting technology for preparing tissue engineering scaffolds.

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Hybrid Tissue Engineering Scaffolds by Combination of Three-Dimensional Printing and Cell Photoencapsulation

Journal of Nanotechnology in Engineering and Medicine ◽

10.1115/1.4031466 ◽

2015 ◽

Vol 6 (2) ◽

Cited By ~ 36

Author(s):

Marica Markovic ◽

Jasper Van Hoorick ◽

Katja Hölzl ◽

Maximilian Tromayer ◽

Peter Gruber ◽

...

Keyword(s):

Tissue Engineering ◽

Fused Deposition Modeling ◽

Structural Parameters ◽

Three Dimensional ◽

Poly Lactic Acid ◽

Tissue Engineering Scaffolds ◽

Ecm Remodeling ◽

Initial Cell ◽

Fused Deposition ◽

Hydrogel Matrix

Three-dimensional (3D) printing offers versatile possibilities for adapting the structural parameters of tissue engineering scaffolds. However, it is also essential to develop procedures allowing efficient cell seeding independent of scaffold geometry and pore size. The aim of this study was to establish a method for seeding the scaffolds using photopolymerizable cell-laden hydrogels. The latter facilitates convenient preparation, and handling of cell suspension, while distributing the hydrogel precursor throughout the pores, before it is cross-linked with light. In addition, encapsulation of living cells within hydrogels can produce constructs with high initial cell loading and intimate cell-matrix contact, similar to that of the natural extra-cellular matrix (ECM). Three dimensional scaffolds were produced from poly(lactic) acid (PLA) by means of fused deposition modeling. A solution of methacrylamide-modified gelatin (Gel-MOD) in cell culture medium containing photoinitiator Li-TPO-L was used as a hydrogel precursor. Being an enzymatically degradable derivative of natural collagen, gelatin-based matrices are biomimetic and potentially support the process of cell-induced remodeling. Preosteoblast cells MC3T3-E1 at a density of 10 × 106 cells per 1 mL were used for testing the seeding procedure and cell proliferation studies. Obtained results indicate that produced constructs support cell survival and proliferation over extended duration of our experiment. The established two-step approach for scaffold seeding with the cells is simple, rapid, and is shown to be highly reproducible. Furthermore, it enables precise control of the initial cell density, while yielding their uniform distribution throughout the scaffold. Such hybrid tissue engineering constructs merge the advantages of rigid 3D printed constructs with the soft hydrogel matrix, potentially mimicking the process of ECM remodeling.

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A Nanofiber Mat With Dual Bioactive Components and a Biomimetic Matrix Structure for Improving Osteogenesis Effect

Frontiers in Chemistry ◽

10.3389/fchem.2021.740191 ◽

2021 ◽

Vol 9 ◽

Author(s):

Yadi Han ◽

Xiaofeng Shen ◽

Sihao Chen ◽

Xiuhui Wang ◽

Juan Du ◽

...

Keyword(s):

Tissue Engineering ◽

Lactic Acid ◽

Bone Repair ◽

Three Dimensional ◽

Dimensional Structure ◽

Bioactive Components ◽

Tissue Engineering Scaffolds ◽

Cell Growth And Migration ◽

Nanofiber Mats

The challenge of effectively regenerating bone tissue through tissue engineering technology is that most tissue engineering scaffolds cannot imitate the three-dimensional structure and function of the natural extracellular matrix. Herein, we have prepared the poly(L-lactic acid)–based dual bioactive component reinforced nanofiber mats which were named as poly(L-lactic acid)/bovine serum albumin/nanohydroxyapatite (PLLA/BSA/nHAp) with dual bioactive components by combining homogeneous blending and electrospinning technology. The results showed that these nanofiber mats had sufficient mechanical properties and a porous structure suitable for cell growth and migration. Furthermore, the results of cell experiments in vitro showed that PLLA/BSA/nHAp composite nanofiber mat could preferably stimulate the proliferation of mouse osteoblastic cells (MC3T3 cells) compared with pure PLLA nanofiber mats. Based on these results, the scaffolds developed in this study are considered to have a great potential to be adhibited as bone repair materials.

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