Three-Dimensional Spheroid Culture on Polymer-Coated Surface Potentiate Stem Cell Functions via Enhanced Cell–Extracellular Matrix Interactions

2020 ◽  
Vol 6 (4) ◽  
pp. 2240-2250
Author(s):  
Seung Jung Yu ◽  
Goro Choi ◽  
Youngbin Cho ◽  
Minseok Lee ◽  
Younghak Cho ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Three Dimensional ◽  
Spheroid Culture ◽  
Cell Functions ◽  
Matrix Interactions ◽  
Coated Surface ◽  
Extracellular Matrix Interactions

scholarly journals Modulation of Inherent Niches in 3D Multicellular MSC Spheroids Reconfigures Metabolism and Enhances Therapeutic Potential

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2747
Author(s):  
Li-Chi Chen ◽  
Hsin-Wen Wang ◽  
Chieh-Cheng Huang
Keyword(s):  
Stem Cell ◽  
Therapeutic Potential ◽  
Three Dimensional ◽  
Paracrine Signaling ◽  
Multicellular Spheroids ◽  
Spheroid Culture ◽  
Matrix Interactions ◽  
Extensive Cell ◽  
Cellular Behaviors ◽  
Extracellular Matrix Interactions

Multicellular spheroids show three-dimensional (3D) organization with extensive cell–cell and cell–extracellular matrix interactions. Owing to their native tissue-mimicking characteristics, mesenchymal stem cell (MSC) spheroids are considered promising as implantable therapeutics for stem cell therapy. Herein, we aim to further enhance their therapeutic potential by tuning the cultivation parameters and thus the inherent niche of 3D MSC spheroids. Significantly increased expression of multiple pro-regenerative paracrine signaling molecules and immunomodulatory factors by MSCs was observed after optimizing the conditions for spheroid culture. Moreover, these alterations in cellular behaviors may be associated with not only the hypoxic niche developed in the spheroid core but also with the metabolic reconfiguration of MSCs. The present study provides efficient methods for manipulating the therapeutic capacity of 3D MSC spheroids, thus laying solid foundations for future development and clinical application of spheroid-based MSC therapy for regenerative medicine.

A rhabdomyosarcoma hydrogel model to unveil cell-extracellular matrix interactions

2021 ◽  
Author(s):  
Mattia Saggioro ◽  
Stefania D'Agostino ◽  
Anna Gallo ◽  
Sara Crotti ◽  
Sara D'Aronco ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Cell Culture ◽  
Culture Systems ◽  
Matrix Interactions ◽  
In Vitro Systems ◽  
Extracellular Matrix Interactions

Three-dimensional (3D) culture systems are progressively getting attention given their potential in overcoming limitations of the classical 2D in vitro systems. Among different supports for 3D cell culture, hydrogels (HGs)...

ROS stimulate reorganization of mesangial cell-collagen gels by tyrosine kinase signaling

1999 ◽  
Vol 276 (2) ◽  
pp. F278-F287 ◽  
Author(s):  
Roy Zent ◽  
Menachem Ailenberg ◽  
Gregory P. Downey ◽  
Melvin Silverman
Keyword(s):  
Extracellular Matrix ◽  
Tyrosine Phosphorylation ◽  
Mesangial Cell ◽  
Mesangial Cells ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Kinase Signaling ◽  
Matrix Interactions ◽  
Three Dimensional Culture ◽  
Extracellular Matrix Interactions

Reactive oxygen species (ROS) initiate multiple pathological and physiological cellular responses, including tyrosine phosphorylation of proteins. In this study, we investigated the effects of ROS on cell-extracellular matrix interactions utilizing the floating three-dimensional collagen gel assay. Exposure of mesangial cells grown in three-dimensional culture to H2O2, 3-amino-1,2,4-triazole (a catalase inhibitor), or puromycin is associated with gel reorganization accompanied by tyrosine phosphorylation of multiple proteins, including focal adhesion kinase (FAK). Neutrophils cocultured with mesangial cells in three-dimensional culture also induce mesangial cell-collagen gel reorganization and initiate tyrosine phosphorylation of a similar set of proteins. Collectively, these results show that ROS of either endogenous or exogenous origin can modulate mesangial cell-extracellular matrix interactions through initiation of a phosphotyrosine kinase signaling cascade. Consequently, ROS may play a role as signaling molecules that regulate mesangial cell-extracellular matrix interactions in both physiological and pathological conditions.

scholarly journals Structural analysis of receptors and actin polarity in platelet protrusions

2021 ◽  
Vol 118 (37) ◽  
pp. e2105004118
Author(s):  
Simona Sorrentino ◽  
Jose Javier Conesa ◽  
Ana Cuervo ◽  
Roberto Melero ◽  
Bruno Martins ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Structural Analysis ◽  
Structural Information ◽  
Three Dimensional ◽  
Cell Spreading ◽  
Membrane Receptors ◽  
Structural Class ◽  
Matrix Interactions ◽  
Contractile Forces ◽  
Extracellular Matrix Interactions

During activation the platelet cytoskeleton is reorganized, inducing adhesion to the extracellular matrix and cell spreading. These processes are critical for wound healing and clot formation. Initially, this task relies on the formation of strong cellular–extracellular matrix interactions, exposed in subendothelial lesions. Despite the medical relevance of these processes, there is a lack of high-resolution structural information on the platelet cytoskeleton controlling cell spreading and adhesion. Here, we present in situ structural analysis of membrane receptors and the underlying cytoskeleton in platelet protrusions by applying cryoelectron tomography to intact platelets. We utilized three-dimensional averaging procedures to study receptors at the plasma membrane. Analysis of substrate interaction-free receptors yielded one main structural class resolved to 26 Å, resembling the αIIbβ3 integrin folded conformation. Furthermore, structural analysis of the actin network in pseudopodia indicates a nonuniform polarity of filaments. This organization would allow generation of the contractile forces required for integrin-mediated cell adhesion.

Sign in / Sign up

Export Citation Format

Share Document