scholarly journals Dehydration Enhances Prebiotic Lipid Remodeling and Vesicle Formation in Acidic Environments

2022 ◽  
Author(s):  
Luke H. Steller ◽  
Martin J. Van Kranendonk ◽  
Anna Wang
Keyword(s):  
Vesicle Formation ◽  
Acidic Environments

scholarly journals Dehydration Enhances Prebiotic Lipid Remodeling and Vesicle Formation in Acidic Environments

2021 ◽  
Author(s):  
Luke H Steller ◽  
Martin J Van Kranendonk ◽  
Anna Wang
Keyword(s):  
Lipid Bilayer ◽  
Building Blocks ◽  
Selective Advantage ◽  
Physicochemical Process ◽  
Vesicle Formation ◽  
Acidic Conditions ◽  
Acidic Environments ◽  
Thin Walled ◽  
Lipid Aggregates

The encapsulation of genetic polymers inside lipid bilayer compartments is a vital step in the emergence of cell-based life. However, even though acidic conditions promote many reactions required for generating prebiotic building blocks, prebiotically-relevant lipids tend to form denser aggregates at acidic pHs rather than prebiotically useful vesicles that exhibit sufficient solute encapsulation. Here we describe how dehydration/rehydration (DR) events, a prebiotically-relevant physicochemical process known to promote polymerization reactions, can remodel dense lipid aggregates into thin-walled vesicles capable of RNA encapsulation even at acidic pHs. Furthermore, DR events appears to favor the encapsulation of RNA within thin-walled vesicles over more lipid-rich vesicles, thus conferring such vesicles a selective advantage.

Corrosion resistance and mechanical properties of quenched and tempered 28MnCrB5 steel in two acidic environments

2017 ◽  
Vol 59 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Aysel Yazıcı ◽  
M. Sadrettin Zeybek ◽  
Hüseyin Güler ◽  
Ahmet Murat Pınar ◽  
Renas Tücer
Keyword(s):  
Mechanical Properties ◽  
Corrosion Resistance ◽  
Acidic Environments

Intracellular trafficking and cytoplasmic streaming under abiotic stress conditions

2019 ◽  
Vol 6 (04) ◽  
Author(s):  
JESHIMA KHAN YASIN ◽  
ANIL KUMAR SINGH
Keyword(s):  
Plasma Membrane ◽  
Abiotic Stress ◽  
Confocal Microscopy ◽  
Fusion Protein ◽  
Intracellular Trafficking ◽  
Cytoplasmic Streaming ◽  
Living Cells ◽  
Stress Conditions ◽  
Vesicle Formation ◽  
External Stimuli

Cytoplasmic streaming is one among the vital activities of the living cells. In plants cytolplasmic streaming could clearly be seen in hypocotyls of growing seedlings. To observe cytoplsmic streaming and its correlated intracellular trafficking an investigation was conducted in legumes in comparison with GFP-AtRab75 and 35S::GFP:δTIP tonoplast fusion protein expressing arabidopsis lines. These seedlings were observed under confocal microscopy with different buffer incubation treatments and under different stress conditions. GFP expressing 35S::GFP:δTIP tonoplast lines were looking similar to the control lines and differ under stress conditions. Movement of cytoplasmic invaginations within the tonoplast and cytoplasmic sub vesicle or bulb budding during cytoplasmic streaming was observed in hypocotyls of At-GFP tonoplast plants. We found the cytoplasmic bulbs/ vesicles or sub vesicle formation from the plasma membrane. The streaming speed also depends on the incubation medium in which the specimen was incubated, indicating that the external stimuli as well as internal stimuli can alter the speed of streaming

scholarly journals In Vitro Incorporation of GPI-Anchored Proteins Into Human Erythrocytes and Their Fate in the Membrane

Blood ◽  
1998 ◽  
Vol 91 (5) ◽  
pp. 1784-1792 ◽  
Author(s):  
Gianluca Civenni ◽  
Samuel T. Test ◽  
Urs Brodbeck ◽  
Peter Bütikofer
Keyword(s):  
Membrane Vesicle ◽  
Human Erythrocytes ◽  
Membrane Extraction ◽  
Membrane Microdomains ◽  
Membrane Insertion ◽  
Vesicle Formation ◽  
Calcium Loading ◽  
Lipid Moiety ◽  
Triton X

Abstract In many different cells, glycosylphosphatidylinositol (GPI)-anchored molecules are clustered in membrane microdomains that resist extraction by detergents at 4°C. In this report, we identified the presence of such domains in human erythrocytes and examined the ability of exogenously-added GPI-anchored molecules to colocalize with the endogenous GPI-anchored proteins in these detergent-insoluble complexes. We found that the addition to human erythrocytes of three purified GPI-anchored proteins having different GPI lipid moieties resulted in their efficient and correct incorporation into the membrane. The extent of membrane insertion was dependent on the intactness of the GPI lipid moiety. However, unlike the endogenous GPI-anchored proteins, the in vitro incorporated GPI molecules were not resistant to membrane extraction by Triton X-100 at 4°C. In addition, in contrast to the endogenous GPI-anchored proteins, they were not preferentially released from erythrocytes during vesiculation induced by calcium loading of the cells. These results suggest that in vitro incorporated GPI-linked molecules are excluded from pre-existing GPI-enriched membrane areas in human erythrocytes and that these microdomains may represent the sites of membrane vesicle formation.

scholarly journals Mutational analysis of caveolin-induced vesicle formation

FEBS Letters ◽  
1998 ◽  
Vol 434 (1-2) ◽  
pp. 127-134 ◽  
Author(s):  
Shengwen Li ◽  
Ferruccio Galbiati ◽  
Daniela Volonte' ◽  
Massimo Sargiacomo ◽  
Jeffrey A Engelman ◽  
...  
Keyword(s):  
Mutational Analysis ◽  
Vesicle Formation

Development of a facile one-pot synthesis method for an ingestible pH sensitive actuator

MRS Advances ◽  
2019 ◽  
Vol 5 (17) ◽  
pp. 881-889
Author(s):  
Alex Keller ◽  
Holly Warren ◽  
Marc in het Panhuis
Keyword(s):  
Acrylic Acid ◽  
Calcium Hydroxide ◽  
Sodium Citrate ◽  
Synthesis Method ◽  
Gastric Fluid ◽  
Ph Sensitive ◽  
One Pot ◽  
Acidic Environments ◽  
Printed Materials ◽  
Poly Acrylic Acid

ABSTRACTEdible devices are an emergent technology and in this paper the simplicity and efficacy that poly(acrylic acid)/calcium hydroxide possess in creating a pH sensitive ingestible actuator which responds to acidic environments such as gastric fluid is demonstrated. It was found that poly(acrylic acid)/calcium hydroxide hydrogels exhibit reversible actuation upon submerging in 0.1 M sodium citrate for 2 hours. Our results show that these hydrogels can restore their compressive stress to 0.19 ± 0.06 MPa, swelling ratio to 26 ± 2 and volume to 56% ± 3% of its original volume. This work offers new possibilities for developments in a variety of fields such as drug delivery, 4D printed materials, soft robotics and edible devices.

Vesicle formation in the Golgi apparatus

1989 ◽  
Vol 141 (4) ◽  
pp. 463-504 ◽  
Author(s):  
George F. Oster ◽  
Louis Y. Cheng ◽  
Hsiao-Ping H. Moore ◽  
Alan S. Perelson
Keyword(s):  
Golgi Apparatus ◽  
Vesicle Formation

IL-25 Hermansky-Pudlak syndrome: a model for abnormal vesicle formation and trafficking

2003 ◽  
Vol 16 (5) ◽  
pp. 584-584 ◽  
Author(s):  
M. Huizing ◽  
A. Helip-Wooley ◽  
H. Dorward ◽  
D. Claassen ◽  
R. Hess ◽  
...  
Keyword(s):  
Vesicle Formation ◽  
Hermansky Pudlak Syndrome
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