scholarly journals Direct Detection of Conserved Viral Sequences and Other Nucleic Acid Motifs with Solid-State Nanopores

ACS Nano ◽  
2021 ◽  
Author(s):  
Komal Sethi ◽  
Gabrielle P. Dailey ◽  
Osama K. Zahid ◽  
Ethan W. Taylor ◽  
Jan A. Ruzicka ◽  
...  
Keyword(s):  
Solid State ◽  
Nucleic Acid ◽  
Direct Detection ◽  
Viral Sequences

scholarly journals Picomolar Fingerprinting of Nucleic Acid Nanoparticles Using Solid-State Nanopores

ACS Nano ◽  
2017 ◽  
Vol 11 (10) ◽  
pp. 9701-9710 ◽  
Author(s):  
Mohammad Amin Alibakhshi ◽  
Justin R. Halman ◽  
James Wilson ◽  
Aleksei Aksimentiev ◽  
Kirill A. Afonin ◽  
...  
Keyword(s):  
Solid State ◽  
Nucleic Acid

scholarly journals Universal amplification-free molecular diagnostics by billion-fold hierarchical nanofluidic concentration

2019 ◽  
Vol 116 (33) ◽  
pp. 16240-16249 ◽  
Author(s):  
Wei Ouyang ◽  
Jongyoon Han
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Molecular Diagnostics ◽  
Troponin I ◽  
Direct Detection ◽  
Signal To Noise Ratio ◽  
Protein Detection ◽  
Clinical Diagnostics ◽  
Nucleic Acid Amplification ◽  
Enzyme Linked Immunosorbent Assay

Rapid and reliable detection of ultralow-abundance nucleic acids and proteins in complex biological media may greatly advance clinical diagnostics and biotechnology development. Currently, nucleic acid tests rely on enzymatic processes for target amplification (e.g., PCR), which have many inherent issues restricting their implementation in diagnostics. On the other hand, there exist no protein amplification techniques, greatly limiting the development of protein-based diagnosis. We report a universal biomolecule enrichment technique termed hierarchical nanofluidic molecular enrichment system (HOLMES) for amplification-free molecular diagnostics using massively paralleled and hierarchically cascaded nanofluidic concentrators. HOLMES achieves billion-fold enrichment of both nucleic acids and proteins within 30 min, which not only overcomes many inherent issues of nucleic acid amplification but also provides unprecedented enrichment performance for protein analysis. HOLMES features the ability to selectively enrich target biomolecules and simultaneously deplete nontargets directly in complex crude samples, thereby enormously enhancing the signal-to-noise ratio of detection. We demonstrate the direct detection of attomolar nucleic acids in urine and serum within 35 min and HIV p24 protein in serum within 60 min. The performance of HOLMES is comparable to that of nucleic acid amplification tests and near million-fold improvement over standard enzyme-linked immunosorbent assay (ELISA) for protein detection, being much simpler and faster in both applications. We additionally measured human cardiac troponin I protein in 9 human plasma samples, and showed excellent agreement with ELISA and detection below the limit of ELISA. HOLMES is in an unparalleled position to unleash the potential of protein-based diagnosis.

scholarly journals Exploration of solid-state nanopores in characterizing reaction mixtures generated from a catalytic DNA assembly circuit

2019 ◽  
Vol 10 (7) ◽  
pp. 1953-1961 ◽  
Author(s):  
Zhentong Zhu ◽  
Ruiping Wu ◽  
Bingling Li
Keyword(s):  
Solid State ◽  
Nucleic Acid ◽  
Base Pair ◽  
Quantitative Method ◽  
Dna Assembly ◽  
Characterization Methods ◽  
Catalytic Dna ◽  
Acid Amplifier

We adapt a solid-state nanopore for analyzing DNA assembly mixtures, which is usually a tougher task for either traditional characterization methods or nanopores themselves. A trigger induced nucleic acid amplifier, SP-CHA, is designed as a model. We propose an electrophoresis-gel like, but homogeneous, quantitative method that can comprehensively profile the “base-pair distribution” of SP-CHA concatemer mixtures.

Direct Detection of3hJNC′Hydrogen-Bond Scalar Couplings in Proteins by Solid-State NMR Spectroscopy

2009 ◽  
Vol 48 (49) ◽  
pp. 9322-9325 ◽  
Author(s):  
Paul Schanda ◽  
Matthias Huber ◽  
René Verel ◽  
Matthias Ernst ◽  
Beat H. Meier
Keyword(s):  
Hydrogen Bond ◽  
Solid State ◽  
Nmr Spectroscopy ◽  
Solid State Nmr ◽  
Direct Detection ◽  
Scalar Couplings ◽  
Solid State Nmr Spectroscopy

Distinguishing Single- and Double-Stranded Nucleic Acid Molecules Using Solid-State Nanopores

Nano Letters ◽  
2009 ◽  
Vol 9 (8) ◽  
pp. 2953-2960 ◽  
Author(s):  
Gary M. Skinner ◽  
Michiel van den Hout ◽  
Onno Broekmans ◽  
Cees Dekker ◽  
Nynke H. Dekker
Keyword(s):  
Solid State ◽  
Nucleic Acid
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