A Genetically Encoded Bioluminescent System for Fast and Highly Sensitive Detection of Antibodies with a Bright Green Fluorescent Protein

ACS Nano ◽  
2021 ◽  
Author(s):  
Mengying Deng ◽  
Jing Yuan ◽  
Haibin Yang ◽  
Xuli Wu ◽  
Xiaoyuan Wei ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Bright Green ◽  
Green Fluorescent ◽  
Highly Sensitive Detection

scholarly journals Distribution pattern of GFP (green fluorescent protein) in a bivalve-inhabiting hydrozoan, Eutima japonica (Leptomedusae: Eirenidae)

2010 ◽  
Vol 90 (7) ◽  
pp. 1371-1374
Author(s):  
Shin Kubota ◽  
Eriko Nomaru ◽  
Hiroko Uchida ◽  
Akio Murakami
Keyword(s):  
Green Fluorescent Protein ◽  
Distribution Pattern ◽  
Fluorescence Spectra ◽  
Fluorescent Protein ◽  
Bright Green ◽  
The Common ◽  
Green Fluorescent ◽  
Auto Fluorescence ◽  
Japan And China

Bright green auto-fluorescence was observed in the umbrellar margin, umbrellar marginal warts, tentacular bulbs, tentacles, and manubrium of laboratory-reared immature (1–14 days old) medusae of Eutima japonica from Japan and China. In vivo microscopic fluorescence spectra showed that the green fluorescence was similar to that of green fluorescent protein (GFP) found in Aequorea victoria, although the maximum emission wavelength (503 nm) was slightly bluer. No fluorescence was detected in the cirri, statocysts, radial canals, velum, or subumbrella of the medusae. The fluorescence distribution pattern in E. japonica more closely resembles that of Eugymnanthea inquilina from the Mediterranean Sea than that of Japanese Eugymnanthea japonica, which is the derived species of E. japonica. This suggests that the common fluorescence pattern is convergently evolved in the former two species, perhaps owing to the as yet unclarified physiological and/or ecological function of GFP and/or GFP-like proteins.

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