Contrast-Enhanced X-ray Detection of Microcalcifications in Radiographically Dense Mammary Tissue Using Targeted Gold Nanoparticles

Quantitative cellular in vitro nanoparticle uptake measurements are possible with a large number of different techniques, however, all have their respective restrictions. Here, we demonstrate the application of synchrotron-based X-ray fluorescence imaging (XFI) on prostate tumor cells, which have internalized differently functionalized gold nanoparticles. Total nanoparticle uptake on the order of a few hundred picograms could be conveniently observed with microsamples consisting of only a few hundreds of cells. A comparison with mass spectroscopy quantification is provided, experimental results are both supported and sensitivity limits of this XFI approach extrapolated by Monte-Carlo simulations, yielding a minimum detectable nanoparticle mass of just 5 pg. This study demonstrates the high sensitivity level of XFI, allowing non-destructive uptake measurements with very small microsamples within just seconds of irradiation time.

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Contrast enhanced ultrasound as a new tool to estimate the performance of midline catheters in the single patient

The Journal of Vascular Access ◽

10.1177/11297298211034629 ◽

2021 ◽

pp. 112972982110346

Author(s):

Antonio Gidaro ◽

Francesco Casella ◽

Francesca Lugli ◽

Chiara Cogliati ◽

Maria Calloni ◽

...

Keyword(s):

Contrast Enhanced Ultrasound ◽

Single Patient ◽

Central Venous ◽

X Ray ◽

Chamber View ◽

Contrast Enhanced ◽

Chest X Ray ◽

Vein Diameter ◽

Tip Position ◽

Echocardiographic Images

Background: Contrast enhanced ultrasound (CEUS) through MicroBubbles Time (MBT) (time from infusion of saline with addition of micro-bubbles of air to visualization of first bubbles in right atrium (RA), visualized by subxiphoid or apical echocardiography) is an alternative to Intracavitary ECG and chest X-ray in evaluation of tip location in central venous catheters. Objective: To evaluate feasibility and variability of CEUS in peripheral catheters (Midline-MC) in a cohort of patients and in a subgroup where tip location was also performed through chest X-ray. Secondary outcomes were verifying the correlation between MBT and distance between tip of MC and RA (anthropometric and radiological measures), body mass index (BMI), vein diameter at point of insertion. Methods: Patients with insertion of MC were enrolled in this prospective cohort. After catheter insertion, CEUS was performed recording MBT. Results: One hundred thirty-two MCs were inserted, 45 performed Chest X-ray. MBT wasn’t feasible in 7 (5%) because of low quality echocardiographic images. Subcostal view was available in 114 patients (91.2%), while 11 patients (8.8%) were examined through apical four-chamber view. Mean MBT in the whole population was 2.3 ± 0.8 s. Significant correlation between anthropometric and radiological measures, BMI and MBT was found. 32.8% of MC had a MBT ⩽2 s. Conclusions: CEUS could be useful to estimate tip position. Our study showed how 2 s is not a suitable cutoff to confirm central catheter’s tip.

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Quantification of tumor angiogenesis with contrast-enhanced x-ray imaging in preclinical studies: a review

Biomedical Physics & Engineering Express ◽

10.1088/2057-1976/aadc2d ◽

2018 ◽

Vol 4 (6) ◽

pp. 062001 ◽

Cited By ~ 3

Author(s):

L Ayala-Domínguez ◽

M E Brandan

Keyword(s):

Tumor Angiogenesis ◽

Preclinical Studies ◽

X Ray ◽

X Ray Imaging ◽

Contrast Enhanced

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Green Synthesis of Silver and Gold Nanoparticles via Sargassum serratifolium Extract for Catalytic Reduction of Organic Dyes

Catalysts ◽

10.3390/catal11030347 ◽

2021 ◽

Vol 11 (3) ◽

pp. 347

Author(s):

Beomjin Kim ◽

Woo Chang Song ◽

Sun Young Park ◽

Geuntae Park

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Catalytic Reduction ◽

Organic Dyes ◽

Low Cost ◽

Inorganic Nanoparticles ◽

X Ray ◽

Selected Area Electron Diffraction ◽

Transmission Electron ◽

Sargassum Serratifolium

The green synthesis of inorganic nanoparticles (NPs) using bio-materials has attained enormous attention in recent years due to its simple, eco-friendly, low-cost and non-toxic nature. In this work, silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were synthesized by the marine algae extract, Sargassum serratifolium (SS). The characteristic studies of bio-synthesized SS-AgNPs and SS-AuNPs were carried out by using ultraviolet–visible (UV–Vis) absorption spectroscopy, dynamic light scattering (DLS), high-resolution transmission electron microscope (HR-TEM), selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). Phytochemicals in the algae extract, such as meroterpenoids, acted as a capping agent for the NPs’ growth. The synthesized Ag and Au NPs were found to have important catalytic activity for the degradation of organic dyes, including methylene blue, rhodamine B and methyl orange. The reduction of dyes by SS-AgNPs and -AuNPs followed the pseudo-first order kinetics.

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Synthesis and Study the Properties of a Solar Cell of Gold Nanoparticles Prepared in a Simple Chemical Way

NeuroQuantology ◽

10.14704/nq.2021.19.11.nq21176 ◽

2021 ◽

Vol 19 (11) ◽

pp. 66-71

Author(s):

Maithm A. Obaid ◽

Suha A Fadaam ◽

Osama S. Hashim

Keyword(s):

Electron Microscopy ◽

Gold Nanoparticles ◽

Chemical Method ◽

X Ray Diffraction ◽

Structural And Optical Properties ◽

Casting Method ◽

X Ray ◽

Transmission Electron ◽

Simple Chemical ◽

Scanning Electron

The aim of this study is to prepare gold nanoparticles by a simple chemical method at a temperature of 70°C. The solution was dried on glass basest by Casting method, the rate of five drops per sample At a temperature 100 C. Then the structural and optical properties have been confirmed by X-ray diffraction, scanning electron microscopy (SEM) and Transmission Electron microscope (TEM), Fourier Transform Infrared Spectroscopy (FTIR) and spectrum.

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