Fluorescence Detection of DNA Hybridization Based on the Aggregation-Induced Emission of a Perylene-Functionalized Polymer

2014 ◽  
Vol 6 (14) ◽  
pp. 11136-11141 ◽  
Author(s):  
Guojie Wang ◽  
Ruichen Zhang ◽  
Chen Xu ◽  
Ruyi Zhou ◽  
Jie Dong ◽  
...  
2021 ◽  
Author(s):  
Shenglan Wang ◽  
Chong-Hua Zhang ◽  
Peisheng Zhang ◽  
Shu Chen ◽  
Zhi-ling Song ◽  
...  

Aggregation induced emission (AIE) dots have gained broad attention in fluorescence bioimaging and biosensor in virtue of their distinctive optical properties of splendid biocompatibility, high brightness and good photostability. However,...


2017 ◽  
Vol 38 (6) ◽  
pp. 1600657 ◽  
Author(s):  
Young Kyu Song ◽  
Beomjin Kim ◽  
Tae Hee Lee ◽  
Jin Chul Kim ◽  
Joon Hyun Nam ◽  
...  

1991 ◽  
Vol 37 (9) ◽  
pp. 1506-1512 ◽  
Author(s):  
E F Templeton ◽  
H E Wong ◽  
R A Evangelista ◽  
T Granger ◽  
A Pollak

Abstract A new nonisotopic detection method based on time-resolved fluorescence for nucleic acid hybridization assays with alkaline phosphatase labels has been developed: enzyme-amplified lanthanide luminescence (EALL). EALL combines the amplification of an enzyme label with the sensitivity and background elimination of time-resolved fluorescence detection of lanthanide ion luminescence. The detection system for alkaline phosphatase makes use of a phosphorylated salicylic acid derivative that, upon dephosphorylation, gives a product capable of forming a luminescent terbium chelate. We demonstrate DNA hybridization assays by using two substrates, one for membrane and one for solution-based formats. Using the substrate that produces a more adhesive product allows performance of dot-blot and Southern blot assays on nylon membranes; results can be recorded with a time-resolved photographic camera system, or with an ultraviolet transilluminator-based system. Less than 4 pg of target sequence can be detected in a dot-blot assay after incubation with substrate for 2-4 h. DNA microwell-plate hybridization assays with the more soluble substrate/product pair can be quantified with time-resolved fluorescence plate readers, giving a similar detection sensitivity. EALL is thus a practical time-resolved fluorescence-based alternative to other detection systems for DNA hybridization assays.


The Analyst ◽  
2018 ◽  
Vol 143 (13) ◽  
pp. 3068-3074 ◽  
Author(s):  
Shanliang Song ◽  
Yuping Zhang ◽  
Yizhou Yang ◽  
Chuanxi Wang ◽  
Ying Zhou ◽  
...  

A novel dual-emission fluorescent nanocomposite material, CDs/Cu NCs, was fabricated for detecting trace water in organic solvents (DMSO, DMF, THF, and ACN).


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