Resonance Raman microprobe spectroscopy of rhodopsin mutants: effect of substitutions in the third transmembrane helix

Biochemistry ◽  
1992 ◽  
Vol 31 (22) ◽  
pp. 5105-5111 ◽  
Author(s):  
Steven W. Lin ◽  
Thomas P. Sakmar ◽  
Roland R. Franke ◽  
H. Gobind Khorana ◽  
Richard A. Mathies
Keyword(s):  
Transmembrane Helix ◽  
Resonance Raman ◽  
The Third ◽  
Raman Microprobe

scholarly journals Point Mutations in Transmembrane Helices 2 and 3 of ExbB and TolQ Affect Their Activities in Escherichia coli K-12

2004 ◽  
Vol 186 (13) ◽  
pp. 4402-4406 ◽  
Author(s):  
Volkmar Braun ◽  
Christina Herrmann
Keyword(s):  
Escherichia Coli ◽  
Amino Acid ◽  
Transmembrane Helix ◽  
Point Mutations ◽  
Transmembrane Helices ◽  
The Third ◽  
Form Part ◽  
K 12

ABSTRACT Replacement of glutamate 176, the only charged amino acid in the third transmembrane helix of ExbB, with alanine (E176A) abolished ExbB activity in all determined ExbB-dependent functions of Escherichia coli. Combination of the mutations T148A in the second transmembrane helix and T181A in the third transmembrane helix, proposed to form part of a proton pathway through ExbB, also resulted in inactive ExbB. E176 and T148 are strictly conserved in ExbB and TolQ proteins, and T181 is almost strictly conserved in ExbB, TolQ, and MotA.

What Makes Red Visual Pigments Red? A Resonance Raman Microprobe Study of Retinal Chromophore Structure in Iodopsin

Biochemistry ◽  
1994 ◽  
Vol 33 (8) ◽  
pp. 2151-2160 ◽  
Author(s):  
Steven W. Lin ◽  
Yasushi Imamoto ◽  
Yoshitaka Fukada ◽  
Yoshinori Shichida ◽  
Toru Yoshizawa ◽  
...  
Keyword(s):  
Resonance Raman ◽  
Visual Pigments ◽  
Raman Microprobe ◽  
Retinal Chromophore

Asp147 in the third transmembrane helix of the rat μ opioid receptor forms ion-pairing with morphine and naltrexone

Life Sciences ◽  
1999 ◽  
Vol 65 (2) ◽  
pp. 175-185 ◽  
Author(s):  
Jian-Guo Li ◽  
Chongguang Chen ◽  
Jinling Yin ◽  
Kenner Rice ◽  
Ying Zhang ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Transmembrane Helix ◽  
Ion Pairing ◽  
The Third ◽  
Μ Opioid Receptor

Continuously Tuneable, Quasi-Continuous-Wave Source for Ultraviolet Resonance Raman Spectroscopy

1995 ◽  
Vol 49 (2) ◽  
pp. 247-252 ◽  
Author(s):  
Stephen J. Doig ◽  
Franklyn G. Prendergast
Keyword(s):  
Raman Spectroscopy ◽  
Continuous Wave ◽  
Uv Light ◽  
Resonance Raman Spectroscopy ◽  
Resonance Raman ◽  
Laser Source ◽  
Ultraviolet Region ◽  
Wave Source ◽  
The Third ◽  
Ultraviolet Resonance Raman

A continuously tuneable, quasi-continuous-wave (cw), ultraviolet source for resonance Raman spectroscopy has been developed. The third and fourth harmonics of a picosecond mode-locked titanium: sapphire laser were generated in nonlinear crystals. The pulses which resulted from these processes were spectrally narrow (10–15 cm−1) and had low peak powers (<1 kW), while the system as a whole provides high average powers. Fourth-harmonic wavelengths were generated from 208 to 230 nm with average powers from 15 to 40 mW, while the third harmonic ranged from 255 to 305 nm with over 90 mW of power. The UV light was well collimated and focused well, and the power was stable for hours. Resonance Raman spectra of tryptophan and phenylalanine were recorded to demonstrate the practical application of this system. Spectra with excellent signal-to-noise ratios were recorded in 6 min with no deleterious effects from nonlinear processes. The selective enhancement of tryptophan scattering in a 1:10 molar solution of tryptophan to phenylalanine makes clear the advantage of a continuously tuneable UV source. Elaboration of this system to the full tuning range of titanium: sapphire lasers will create a laser source suitable for resonance Raman spectroscopy throughout the ultraviolet region (205–400 nm).

scholarly journals Mutation of a Conserved Threonine in the Third Transmembrane Helix of α- and β-Connexins Creates a Dominant-negative Closed Gap Junction Channel

2005 ◽  
Vol 281 (12) ◽  
pp. 7994-8009 ◽  
Author(s):  
Derek L. Beahm ◽  
Atsunori Oshima ◽  
Guido M. Gaietta ◽  
Galen M. Hand ◽  
Amy E. Smock ◽  
...  
Keyword(s):  
Gap Junction ◽  
Transmembrane Helix ◽  
Dominant Negative ◽  
The Third ◽  
Gap Junction Channel

Mutation of ASP to glu in the third transmembrane helix of the muscarinic receptors

Life Sciences ◽  
1993 ◽  
Vol 52 (5-6) ◽  
pp. 560 ◽  
Author(s):  
K.M. Page ◽  
C.A.M. Curtis ◽  
E.C. Hulme
Keyword(s):  
Muscarinic Receptors ◽  
Transmembrane Helix ◽  
The Third

F200A substitution in the third transmembrane helix of human cannabinoid CB1 receptor converts AM2233 from receptor agonist to inverse agonist

2006 ◽  
Vol 531 (1-3) ◽  
pp. 41-46 ◽  
Author(s):  
Chun-Pyn Shen ◽  
Jing Chen Xiao ◽  
Helen Armstrong ◽  
William Hagmann ◽  
Tung M. Fong
Keyword(s):  
Receptor Agonist ◽  
Transmembrane Helix ◽  
Inverse Agonist ◽  
Cb1 Receptor ◽  
Cannabinoid Cb1 Receptor ◽  
The Third

Resonance Raman in platinum phthalocyanine

1978 ◽  
Vol 56 (7) ◽  
pp. 976-984 ◽  
Author(s):  
Tzer-Hsiang Huang ◽  
Klaus E. Rieckhoff ◽  
Eva-Maria Voigt
Keyword(s):  
Absorption Maximum ◽  
Lower Energy ◽  
Mixed Solvents ◽  
Resonance Raman ◽  
Visible Region ◽  
Excited Singlet ◽  
Absorption Region ◽  
The Third ◽  
Resonance Raman Spectra ◽  
Platinum Phthalocyanine

The resonance Raman spectra and excitation profiles of platinum phthalocyanine (PtPc) in α-chloronaphthalene (α-ClN) and in mixtures of α-ClN and n-octane were obtained at 295 K with excitation from 660 to 570 nm covering the total lower energy absorption region of PtPc. Eighteen fundamentals and overtone-combinations were observed. Our data show that the third absorption maximum of PtPc in the visible region is actually the (0–1), but not the (0–2) as previously interpreted, transition. The (0–1) bands in the excitation profiles of most resonance-enhanced-vibrations were found to be more intense and frequency shifted compared with their counterparts in absorption. Based on the depolarization ratios, we deduced the symmetry of PtPc in α-ClN in the excited singlets to be D2h, C2v, or D2; no differences were found in the mixed solvents. The small Stokes loss observed in the absorption spectra, as well as the absence of the (0–2) maxima in the excitation profiles, indicate that the equilibrium position of the first excited singlet is only slightly different from that of the ground state.

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