Expression of Recombinant Monomer Hemoglobins (Component IV) from the Marine Annelid Glycera dibranchiata: Evidence for Primary Sequence Positional Regulation of Heme Rotational Disorder

Biochemistry ◽  
1994 ◽  
Vol 33 (34) ◽  
pp. 10337-10344 ◽  
Author(s):  
Steve L. Alam ◽  
David P. Dutton ◽  
James D. Satterlee
Keyword(s):  
Primary Sequence ◽  
Glycera Dibranchiata

scholarly journals The cDNA sequences encoding two components of the polymeric fraction of the intracellular hemoglobin of Glycera dibranchiata.

1990 ◽  
Vol 265 (35) ◽  
pp. 21843-21851
Author(s):  
R S Zafar ◽  
L H Chow ◽  
M S Stern ◽  
J S Scully ◽  
P R Sharma ◽  
...  
Keyword(s):  
Cdna Sequences ◽  
Glycera Dibranchiata ◽  
Polymeric Fraction

scholarly journals Primary Sequence of U-1 Nuclear Ribonucleic Acid of Novikoff Hepatoma Ascites Cells

1974 ◽  
Vol 249 (20) ◽  
pp. 6486-6494
Author(s):  
Ramachandra Reddy ◽  
Tae Suk Ro-Choi ◽  
Dale Henning ◽  
Harris Busch
Keyword(s):  
Ribonucleic Acid ◽  
Primary Sequence ◽  
Novikoff Hepatoma

scholarly journals Structural basis of antigen recognition: crystal structure of duck egg lysozyme

2017 ◽  
Vol 73 (11) ◽  
pp. 910-920 ◽  
Author(s):  
David Brent Langley ◽  
Ben Crossett ◽  
Peter Schofield ◽  
Jenny Jackson ◽  
Mahdi Zeraati ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Mass Spectrometry ◽  
Binding Affinity ◽  
Anas Platyrhynchos ◽  
Antigen Recognition ◽  
Pekin Duck ◽  
Structural Basis ◽  
Primary Sequence ◽  
Duck Egg ◽  
Differential Affinity

Duck egg lysozyme (DEL) is a widely used model antigen owing to its capacity to bind with differential affinity to anti-chicken egg lysozyme antibodies. However, no structures of DEL have so far been reported, and the situation had been complicated by the presence of multiple isoforms and conflicting reports of primary sequence. Here, the structures of two DEL isoforms from the eggs of the commonly used Pekin duck (Anas platyrhynchos) are reported. Using structural analyses in combination with mass spectrometry, non-ambiguous DEL primary sequences are reported. Furthermore, the structures and sequences determined here enable rationalization of the binding affinity of DEL for well documented landmark anti-lysozyme antibodies.

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