Plasma Membrane Cholesterol:  A Possible Barrier to Intracellular Oxygen in Normal and Mutant CHO Cells Defective in Cholesterol Metabolism†

Biochemistry ◽  
2003 ◽  
Vol 42 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Nadeem Khan ◽  
Jiangang Shen ◽  
Ta Yuan Chang ◽  
Catherine C. Chang ◽  
Peter C. W. Fung ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Cho Cells ◽  
Cholesterol Metabolism ◽  
Membrane Cholesterol ◽  
Plasma Membrane Cholesterol

scholarly journals Reprogramming cholesterol metabolism in macrophages and its role in host defense against cholesterol-dependent cytolysins

2022 ◽  
Author(s):  
Min-Sub Lee ◽  
Steven J. Bensinger
Keyword(s):  
Plasma Membrane ◽  
Host Defense ◽  
Mammalian Cells ◽  
Cholesterol Metabolism ◽  
Current Knowledge ◽  
Membrane Integrity ◽  
Cholesterol Homeostasis ◽  
Metabolic Reprogramming ◽  
Membrane Cholesterol ◽  
Plasma Membrane Cholesterol

AbstractCholesterol is a critical lipid for all mammalian cells, ensuring proper membrane integrity, fluidity, and biochemical function. Accumulating evidence indicates that macrophages rapidly and profoundly reprogram their cholesterol metabolism in response to activation signals to support host defense processes. However, our understanding of the molecular details underlying how and why cholesterol homeostasis is specifically reshaped during immune responses remains less well understood. This review discusses our current knowledge of cellular cholesterol homeostatic machinery and introduces emerging concepts regarding how plasma membrane cholesterol is partitioned into distinct pools. We then discuss how proinflammatory signals can markedly reshape the cholesterol metabolism of macrophages, with a focus on the differences between MyD88-dependent pattern recognition receptors and the interferon signaling pathway. We also discuss recent work investigating the capacity of these proinflammatory signals to selectively reshape plasma membrane cholesterol homeostasis. We examine how these changes in plasma membrane cholesterol metabolism influence sensitivity to a set of microbial pore-forming toxins known as cholesterol-dependent cytolysins that specifically target cholesterol for their effector functions. We also discuss whether lipid metabolic reprogramming can be leveraged for therapy to mitigate tissue damage mediated by cholesterol-dependent cytolysins in necrotizing fasciitis and other related infections. We expect that advancing our understanding of the crosstalk between metabolism and innate immunity will help explain how inflammation underlies metabolic diseases and highlight pathways that could be targeted to normalize metabolic homeostasis in disease states.

scholarly journals Monoclonal antibody detection of plasma membrane cholesterol microdomains responsive to cholesterol trafficking

2001 ◽  
Vol 42 (9) ◽  
pp. 1492-1500 ◽  
Author(s):  
Howard S. Kruth ◽  
Ina Ifrim ◽  
Janet Chang ◽  
Lia Addadi ◽  
Daniele Perl-Treves ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Plasma Membrane ◽  
Antibody Detection ◽  
Membrane Cholesterol ◽  
Cholesterol Trafficking ◽  
Plasma Membrane Cholesterol

Cell culturing in a three-dimensional matrix affects the localization and properties of plasma membrane cholesterol

2009 ◽  
Vol 33 (10) ◽  
pp. 1079-1086 ◽  
Author(s):  
Nadezhda Stefanova ◽  
Galya Staneva ◽  
Diana Petkova ◽  
Teodora Lupanova ◽  
Roumen Pankov ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Three Dimensional ◽  
Membrane Cholesterol ◽  
Dimensional Matrix ◽  
Cell Culturing ◽  
Plasma Membrane Cholesterol
Sign in / Sign up

Export Citation Format

Share Document