Biodegradable Poly(vinyl alcohol)-polyethylenimine Nanocomposites for Enhanced Gene Expression In Vitro and In Vivo

2011 ◽  
Vol 13 (1) ◽  
pp. 73-83 ◽  
Author(s):  
Ritu Goyal ◽  
Sushil K. Tripathi ◽  
Esther Vazquez ◽  
Pradeep Kumar ◽  
Kailash C. Gupta
Keyword(s):  
Gene Expression ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Biodegradable Poly

Surface Modified Poly(vinyl alcohol) Nanofiber for the Artificial Corneal Stroma

2007 ◽  
Vol 342-343 ◽  
pp. 209-212 ◽  
Author(s):  
Hisatoshi Kobayashi
Keyword(s):  
Corneal Epithelium ◽  
Vinyl Alcohol ◽  
Type I Collagen ◽  
Corneal Stroma ◽  
Light Transmittance ◽  
Poly Vinyl Alcohol ◽  
Type I ◽  
Nanofiber Sheets

Previously we have found that the immobilization of Type I collagen on the poly(vinyl alcohol)(PVA) hydrogel disc was effective in supporting adhesion and growth of the corneal epithelium and stromal cell in vitro. But the durability of the produced corneal epithelium layer in vivo has some problem. We hypothesized the cell construction force is much stronger than the force of the cell adhesion on the flat modified PVA surfaces. Therefore the improvement of mechanical anchoring force between the substrate and formed corneal cell layer maybe become one of the solving methods. In this study, we prepared the PVA nanofiber mat by using the electrospinning method and the surface modification of the PVA nanofiber was studied to improve the durability of the corneal epithelium layer. The collagen-immobilized PVA nanofiber sheets could support the adhesion and proliferation of rabbit corneal epithelial cells. And the stratified corneal epithelium structure was observed on the PVA nanofiber sheets when the epithelium was co-cultured with rabbit corneal stromal cells. It means that the corneal epithelium was well differentiated on the collagen immobilized PVA nanofiber sheet. The stability of the corneal epithelium layer on the PVA was dramatically improved; the stratified epithelium layer was kept for two weeks after the differentiation introduction, totally after one month. A light transmittance of these materials is not yet enough. Further study to improve the transmission of light, is required.

Electrospun poly(vinyl) alcohol/collagen nanofibrous scaffold hybridized by graphene oxide for accelerated wound healing

2018 ◽  
Vol 41 (8) ◽  
pp. 467-473 ◽  
Author(s):  
Rethinam Senthil ◽  
Robert Berly ◽  
Thimmiah Bhargavi Ram ◽  
Nallathambi Gobi
Keyword(s):  
Wound Healing ◽  
Graphene Oxide ◽  
Vinyl Alcohol ◽  
Collagen Type ◽  
Collagen Type I ◽  
Fish Bone ◽  
Poly Vinyl Alcohol ◽  
Type I

Purpose: In this study, a blend of synthetic polymer (poly(vinyl) alcohol), natural polymer (collagen type I from fish bone), and graphene oxide nanoparticles is used to fabricate a composite nanofibrous scaffold, by electrospinning, for their potential application in accelerated wound healing. Methods: The scaffold was characterized for its physicochemical and mechanical properties. In vitro studies were carried out using human keratinocyte cell line (HaCaT) which proved the biocompatibility of the scaffold. In vivo study using mice model was carried out and the healing pattern was evaluated using histopathological studies. Results: Scaffold prepared from poly(vinyl) alcohol, collagen type I from fish bone, and graphene oxide possessed better physicochemical and mechanical properties. In addition, in vivo and in vitro studies showed its accelerated wound healing properties. Conclusion: The scaffold with required strength and biocompatibility may be tried as a wound dressing material in large animals after getting necessary approval.

A novel biodegradable poly(p-dioxanone)-grafted poly(vinyl alcohol) copolymer with a controllable in vitro degradation

Polymer ◽  
2006 ◽  
Vol 47 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Si-Chong Chen ◽  
Zhi-Xuan Zhou ◽  
Yu-Zhong Wang ◽  
Xiu-Li Wang ◽  
Ke-Ke Yang
Keyword(s):  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
In Vitro Degradation ◽  
Biodegradable Poly

Honey-Loaded Egg White/Poly(vinyl alcohol)/Clay Bionanocomposite Hydrogel Wound Dressings: In Vitro and In Vivo Evaluations

2019 ◽  
Vol 28 (1) ◽  
pp. 32-46 ◽  
Author(s):  
Zahra Rafati ◽  
Mohammad Sirousazar ◽  
Zuhair Muhammad Hassan ◽  
Farshad Kheiri
Keyword(s):  
Vinyl Alcohol ◽  
Egg White ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol

In vitro and in vivo evaluation of pirfenidone loaded acrylamide grafted pullulan-poly(vinyl alcohol) interpenetrating polymer networks

2018 ◽  
Vol 202 ◽  
pp. 288-298 ◽  
Author(s):  
Saundray Raj Soni ◽  
Nimmy Kumari ◽  
Bibhas K. Bhunia ◽  
Biswatrish Sarkar ◽  
Biman B. Mandal ◽  
...  
Keyword(s):  
Vinyl Alcohol ◽  
Polymer Networks ◽  
Interpenetrating Polymer Networks ◽  
Poly Vinyl Alcohol ◽  
In Vivo Evaluation

scholarly journals Antimicrobial Films Based on Nanocomposites of Chitosan/Poly(vinyl alcohol)/Graphene Oxide for Biomedical Applications

Biomolecules ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 109 ◽  
Author(s):  
Sebastián Ruiz ◽  
Julián Andrés Tamayo ◽  
Johannes Delgado Ospina ◽  
Diana Paola Navia Porras ◽  
Mayra Eliana Valencia Zapata ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Graphene Oxide ◽  
Vinyl Alcohol ◽  
Biomedical Applications ◽  
Subcutaneous Tissue ◽  
Poly Vinyl Alcohol ◽  
Salmonella Spp ◽  
Mueller Hinton Agar

Today, tissue regeneration is one of the greatest challenges in the field of medicine, since it represents hope after accidents or illnesses. Tissue engineering is the science based on improving or restoring tissues and organs. In this work, five formulations of chitosan/poly(vinyl alcohol)/graphene oxide (CS/PVA/GO) nanocomposites were studied for the development of biodegradable films with potential biomedical applications. The characterization of the films consisted of Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). The antibacterial activity was evaluated in vitro against Gram-positive bacteria Bacillus cereus and Staphylococcus aureus and Gram-negative Salmonella spp. and Escherichia coli, by contact of the film above inoculum bacterial in Müeller–Hinton agar. On the other hand, in vivo tests in which the material implanted in the subcutaneous tissue of Wistar rats demonstrated that the formulation CS/PVA/GO (14.25:85:0.75) was the best antibacterial film with adequate degradation in vivo. All together, these results indicate the potential of the films using nanocomposites of CS/PVA/GO in tissue engineering and cell regeneration.

scholarly journals Új, laparoszkópos beültetésre alkalmas sérvháló állatkísérletes vizsgálata

2016 ◽  
Vol 157 (5) ◽  
pp. 180-184 ◽  
Author(s):  
Péter Marcell Guba
Keyword(s):  
Vinyl Alcohol ◽  
Three Dimensional ◽  
Major Complication ◽  
Biological Properties ◽  
Poly Vinyl Alcohol ◽  
In Vitro Tests ◽  
In Vivo Tests ◽  
Male Wistar Rats

Introduction: Reconstruction of the abdominal wall with mesh is a widely used surgical procedure. The non-absorbable meshes tend to cause numerous side-effects. Aim: The aim of the author was to produce an absorbable, polymer-based mesh that possesses appropriate chemical, mechanical and biological properties. Method: A three-dimensional, biocompatible mesh was produced from poly-vinyl-alcohol using reactive electrospinning. Toxicity and cell-mesh interactions were tested using human lung carcinoma epithelial cells (A-549), and in vivo tests were conducted in 42 male Wistar rats at the 1–5, 7 and 14 postoperative days (3 rats/groups). Results: In the in vitro tests poly-vinyl-alcohol was biocompatible. In the in vivo tests no major complication was associated with the mesh made of poly-vinyl-alcohol. Conclusions: The author concludes that this polymer mesh is biocompatible, it does not damage the surrounding tissues and integrates well with them. Orv. Hetil., 2016, 157(5), 180–184.

Bi-Layer Composite Dressing of Gelatin Nanofibrous Mat and Poly Vinyl Alcohol Hydrogel for Drug Delivery and Wound Healing Application:In-Vitro and In-Vivo Studies

2013 ◽  
Vol 9 (9) ◽  
pp. 1495-1508 ◽  
Author(s):  
Maneesh Jaiswal ◽  
Asheesh Gupta ◽  
Ashwini K. Agrawal ◽  
Manjeet Jassal ◽  
Amit Kr. Dinda ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Wound Healing ◽  
Vinyl Alcohol ◽  
Poly Vinyl Alcohol ◽  
Layer Composite
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