Enzymatically cross-linked hydrogels based on a linear poly(ethylene glycol) analogue for controlled protein release and 3D cell culture

2018 ◽  
Vol 6 (38) ◽  
pp. 6067-6079 ◽  
Author(s):  
Danni Wang ◽  
Xiaowei Yang ◽  
Qiong Liu ◽  
Lin Yu ◽  
Jiandong Ding
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
3D Cell Culture ◽  
Protein Release ◽  
Poly Ethylene Glycol ◽  
Delivery Platform ◽  
Linear Poly ◽  
Poly Ethylene

An injectable PEG-based hydrogel is designed using an enzyme as a catalyst, and its potential as a delivery platform for proteins and cells is demonstrated.

scholarly journals Effects of the poly(ethylene glycol) hydrogel crosslinking mechanism on protein release

2016 ◽  
Vol 4 (3) ◽  
pp. 405-411 ◽  
Author(s):  
Soah Lee ◽  
Xinming Tong ◽  
Fan Yang
Keyword(s):  
Ethylene Glycol ◽  
Differential Effect ◽  
Protein Release ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Poly(ethylene glycol) hydrogel crosslinking mechanism has a differential effect on controlling protein release.

Best of both worlds: Diels–Alder chemistry towards fabrication of redox-responsive degradable hydrogels for protein release

RSC Advances ◽  
2016 ◽  
Vol 6 (78) ◽  
pp. 74757-74764 ◽  
Author(s):  
Ismail Altinbasak ◽  
Rana Sanyal ◽  
Amitav Sanyal
Keyword(s):  
Ethylene Glycol ◽  
Alder Reaction ◽  
Diels Alder ◽  
Protein Release ◽  
Poly Ethylene Glycol ◽  
Diels Alder Reaction ◽  
Redox Responsive ◽  
Poly Ethylene ◽  
Responsive Hydrogels

Poly(ethylene glycol)-based redox-responsive hydrogels have been preparedviathe Diels–Alder reaction between a furan-containing hydrophilic copolymer and a disulfide-containing bis-maleimide based crosslinker.

Tailorable Cell Culture Platforms from Enzymatically Cross-Linked Multifunctional Poly(ethylene glycol)-Based Hydrogels

2013 ◽  
Vol 14 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Donna J. Menzies ◽  
Andrew Cameron ◽  
Trent Munro ◽  
Ernst Wolvetang ◽  
Lisbeth Grøndahl ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
Max Männel ◽  
Carolin Fischer ◽  
Julian Thiele
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Umbilical Vein ◽  
Polymer Materials ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Impact

Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (µSL). However, common resins for µSL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for µSL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.

scholarly journals Acid‐Cleavable Poly(ethylene glycol) Hydrogels Displaying Protein Release at pH 5

2020 ◽  
Vol 26 (13) ◽  
pp. 2947-2953 ◽  
Author(s):  
Johannes Ewald ◽  
Jan Blankenburg ◽  
Matthias Worm ◽  
Laura Besch ◽  
Ronald E. Unger ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Protein Release ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Bottlebrush-architectured poly(ethylene glycol) as an efficient vector for RNA interference in vivo

2019 ◽  
Vol 5 (2) ◽  
pp. eaav9322 ◽  
Author(s):  
Dali Wang ◽  
Jiaqi Lin ◽  
Fei Jia ◽  
Xuyu Tan ◽  
Yuyan Wang ◽  
...  
Keyword(s):  
Rna Interference ◽  
Ethylene Glycol ◽  
Target Genes ◽  
Area Under The Curve ◽  
Fold Increase ◽  
Small Interfering Rnas ◽  
Poly Ethylene Glycol ◽  
Linear Poly ◽  
Poly Ethylene

Nonhepatic delivery of small interfering RNAs (siRNAs) remains a challenge for development of RNA interference–based therapeutics. We report a noncationic vector wherein linear poly(ethylene glycol) (PEG), a polymer generally considered as inert and safe biologically but ineffective as a vector, is transformed into a bottlebrush architecture. This topology provides covalently embedded siRNA with augmented nuclease stability and cellular uptake. Consisting almost entirely of PEG and siRNA, the conjugates exhibit a ~25-fold increase in blood elimination half-life and a ~19-fold increase in the area under the curve compared with unmodified siRNA. The improved pharmacokinetics results in greater tumor uptake and diminished liver capture. Despite the structural simplicity these conjugates efficiently knock down target genes in vivo without apparent toxic and immunogenic reactions. Given the benign biological nature of PEG and its widespread precedence in biopharmaceuticals, we anticipate the brush polymer–based technology to have a significant impact on siRNA therapeutics.

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