Linear Poly(ethylene imine)-Based Hydrogels for Effective Binding and Release of DNA

Purpose: Poly l-lysine (PLL) has been introduced as a strengthening covering layer for alginate microcapsules which are the most convenient way for cell encapsulation. Some disadvantages of PLL such as high price and low biocompatibility have prompted scientists to find better alternatives. Linear poly ethylene imine (LPEI), thanks to its highly similar structure to PLL, could be considered as a proper cost-effective alternative. In this study LPEI and PLL were compared as covering layers of cell-loaded alginate-LPEI-alginate (cALA) and alginate-PLL-alginate (cAPA) microcapsules. Methods: In addition to the physico-mechanical properties, the encapsulation efficiency, cell survival post encapsulation, cell viability, and cellular metabolic activity within the microcapsules were evaluated using trypan blue, live/dead cell staining, and MTT test, respectively. Results: Physico-mechanical evaluation of the microcapsules revealed that the cell microencapsulation process did not affect their shape, size, and mechanical stability. Although the encapsulation efficiency for cALA and cAPA was not different (P>0.05), cell survival post encapsulation was higher in cALA than in cAPA (P<0.05) which could be the reason for the higher cell viability and also cellular metabolic activity within these microcapsules in comparison to cAPA. Conclusion: Here, based on these results, ALA could be introduced as a preferable alternative to APA for cell encapsulation.

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Dextran-graft-linear poly(ethylene imine)s for gene delivery: Importance of the linking strategy

Carbohydrate Polymers ◽

10.1016/j.carbpol.2014.07.048 ◽

2014 ◽

Vol 113 ◽

pp. 597-606 ◽

Cited By ~ 20

Author(s):

Sofia Ochrimenko ◽

Antje Vollrath ◽

Lutz Tauhardt ◽

Kristian Kempe ◽

Stephanie Schubert ◽

...

Keyword(s):

Gene Delivery ◽

Ethylene Imine ◽

Linear Poly ◽

Poly Ethylene

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Calcium phosphate mineralization with linear poly(ethylene imine): a time-resolved study

Colloid & Polymer Science ◽

10.1007/s00396-011-2403-2 ◽

2011 ◽

Vol 289 (8) ◽

pp. 881-888 ◽

Cited By ~ 19

Author(s):

Andriy Shkilnyy ◽

Stefanie Schöne ◽

Claudia Rumplasch ◽

Annett Uhlmann ◽

Annett Hedderich ◽

...

Keyword(s):

Calcium Phosphate ◽

Ethylene Imine ◽

Time Resolved ◽

Phosphate Mineralization ◽

Linear Poly ◽

Poly Ethylene

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Linear poly(ethylene imine)s: true molar masses, solution properties and conformation

Polymer Chemistry ◽

10.1039/c7py01634d ◽

2017 ◽

Vol 8 (46) ◽

pp. 7169-7179 ◽

Cited By ~ 10

Author(s):

Igor Perevyazko ◽

Alexander S. Gubarev ◽

Lutz Tauhardt ◽

Anatoly Dobrodumov ◽

Georges M. Pavlov ◽

...

Keyword(s):

Gene Therapy ◽

Pivotal Role ◽

Polymer Chemistry ◽

Solution Properties ◽

Ethylene Imine ◽

Linear Poly ◽

Poly Ethylene

In-depth characterization of pharmaceutically relevant polymers plays a pivotal role in many areas, including nanoscience, gene therapy, analytical and polymer chemistry etc.

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