Effect of Flow Rate Pattern on Glucose-6-phosphate Dehydrogenase Synthesis in Fed-Batch Culture of Recombinant Saccharomyces cerevisiae

2003 ◽  
Vol 19 (2) ◽  
pp. 320-324 ◽  
Author(s):  
A.S.M. Miguel ◽  
L.C. Martins das Neves ◽  
M. Vitolo ◽  
A. Pessoa, Jr.
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Culture ◽  
Flow Rate ◽  
Fed Batch ◽  
Recombinant Saccharomyces Cerevisiae ◽  
Fed Batch Culture ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0248382
Author(s):  
Emanuela Palomba ◽  
Valentina Tirelli ◽  
Elisabetta de Alteriis ◽  
Palma Parascandola ◽  
Carmine Landi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Batch Culture ◽  
Time Course ◽  
Reference Model ◽  
Product Yield ◽  
Fed Batch ◽  
Batch Bioreactor ◽  
Biotechnological Processes ◽  
Fed Batch Culture

The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.

Glutathione accumulation in ethanol-stat fed-batch culture of Saccharomyces cerevisiae with a switch to cysteine feeding

2010 ◽  
Vol 87 (1) ◽  
pp. 175-183 ◽  
Author(s):  
Ildar Nisamedtinov ◽  
Kaspar Kevvai ◽  
Kerti Orumets ◽  
Jari J. Rautio ◽  
Toomas Paalme
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Culture ◽  
Fed Batch ◽  
Fed Batch Culture
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