In-Vitro Methods May Offer Alternatives to Animal Testing

1984 ◽  
Vol 62 (46) ◽  
pp. 25-28 ◽  
Author(s):  
RON DAGANI
Keyword(s):  
Animal Testing ◽  
In Vitro Methods ◽  
Alternatives To Animal Testing

In Vitro Methods: Alternatives to Animal Testing

2019 ◽  
pp. 447-457
Author(s):  
Dayane Pifer Luco ◽  
Vânia Rodrigues Leite-Silva ◽  
Heather A.E. Benson ◽  
Patricia Santos Lopes
Keyword(s):  
Animal Testing ◽  
In Vitro Methods ◽  
Alternatives To Animal Testing

Research on the development of alternatives to animal testing for pharmaceutical products and international standardisation

Impact ◽  
2021 ◽  
Vol 2021 (8) ◽  
pp. 44-45
Author(s):  
Hajime Kojima
Keyword(s):  
Research Group ◽  
Pharmaceutical Products ◽  
In Vitro Assays ◽  
Animal Testing ◽  
3 Dimensional ◽  
Alternatives To Animal Testing ◽  
Gene Assay ◽  
Innovative Work ◽  
Regulatory Acceptance

Scientists are working to develop new and innovative alternatives to animal testing that don't rely on the use of animals. Takao Ashikaga, Hajime Kojima and Yoko Hirabayashi are part of JaCVAM which works to promote the use of alternatives to animal testing. The goal is to replace, reduce or refine (3Rs) the use of animal under International harmonization. Hirabayashi is also the representative of a research group that is funded by the AMED and the representative of a research group funded by the MHLW. A challenge the researchers are facing in their quest to ensure the welfare of experimental animals and also ensure the safety of various pharmaceutical and chemicals is the lack of biomarkers to more accurately predict toxicity for regulatory acceptance. This means that without animal testing more costly and complex non-animal methods are required and presents a barrier to the adoption of non-animal methods for international standerisation. As such, there is a need to develop an easy way to obtain a lot of information. Hirabayashi and the team are working on the development of AI that can be used to evaluate the safety of different compounds. The researchers are developing in vitro assays such as ordinary 2-dimensional culture, 3-dimensional culture including organoids or spheroids, reporter gene assay and organ-on-a chip; and in silico assays such as computer toxicology using QSAR and Read Across. The researchers hope that their innovative work will contribute to the 3Rs, benefiting animal welfare for regulatory use.

Integrated Decision-tree Testing Strategies for Skin Corrosion and Irritation with Respect to the Requirements of the EU REACH Legislation

2008 ◽  
Vol 36 (1_suppl) ◽  
pp. 65-74
Author(s):  
Christina Grindon ◽  
Robert Combes ◽  
Mark T.D. Cronin ◽  
David W. Roberts ◽  
John F. Garrod
Keyword(s):  
Alternative Methods ◽  
The European Union ◽  
Testing Strategy ◽  
Animal Testing ◽  
Safety Testing ◽  
Alternatives To Animal Testing ◽  
Testing Strategies ◽  
The Status ◽  
The Eu

Liverpool John Moores University and FRAME recently conducted a research project, sponsored by Defra, on the status of alternatives to animal testing with regard to the European Union REACH (Registration, Evaluation and Authorisation of Chemicals) system for the safety testing and risk assessment of chemicals. The project covered all the main toxicity endpoints associated with the REACH system. This report focuses on how to maximise the use of alternative methods (both in vitro and in silico) for skin corrosion and irritation testing within a tiered testing strategy. It considers the latest developments in in vitro testing, with particular reference to the reconstituted skin models which have now been now been successfully validated and independently endorsed as suitable for both skin corrosivity and irritancy testing within the EU.

scholarly journals Development and use of in vitro alternatives to animal testing by the pharmaceutical industry 1980–2013

2015 ◽  
Vol 4 (5) ◽  
pp. 1297-1307 ◽  
Author(s):  
Jen-Yin Goh ◽  
Richard J. Weaver ◽  
Libby Dixon ◽  
Nicola J. Platt ◽  
Ruth A. Roberts
Keyword(s):  
Pharmaceutical Industry ◽  
Animal Testing ◽  
Genetic Toxicology ◽  
Safety Testing ◽  
Safety Pharmacology ◽  
Alternatives To Animal Testing ◽  
Ich Guidelines ◽  
Preclinical Safety

An analysis of the use ofin vitrotechniques in preclinical safety testing revealed a marked increase to >180 000 tests/year by 2012. Step changes in uptake were notable in the three main areas of ADME, safety pharmacology and genetic toxicology, correlated with relevant ICH guidelines.

In vitro pituitary and thyroid cell proliferation assays and their relevance as alternatives to animal testing

2013 ◽  
Vol 221 ◽  
pp. S151
Author(s):  
Barae Jomaa ◽  
Jac M.M.J.G. Aarts ◽  
Laura H.J. de Haan ◽  
Ad A.C.M. Peijinenburg ◽  
Toine F.H. Bovee ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Thyroid Cell ◽  
Animal Testing ◽  
Alternatives To Animal Testing ◽  
Proliferation Assays

The Overt and Hidden Use of Animal-Derived Products in Alternative Methods for Skin Sensitisation: A Systematic Review

2019 ◽  
Vol 47 (5-6) ◽  
pp. 174-195 ◽  
Author(s):  
Bianca Marigliani ◽  
Felipe Perraro Sehn ◽  
Josemar Vinicius Maiworm Abreu Silva ◽  
Luciene Bottentuit López Balottin ◽  
Elisabeth de Fatima Pires Augusto ◽  
...  
Keyword(s):  
Systematic Review ◽  
Safety Assessment ◽  
Alternative Methods ◽  
Animal Origin ◽  
Animal Testing ◽  
Complete Replacement ◽  
In Vitro Methods ◽  
Skin Sensitisation ◽  
Animal Alternatives

In vitro methods that can replace animal testing in the identification of skin sensitisers are now a reality. However, as cell culture and related techniques usually rely on animal-derived products, these methods may be failing to address the complete replacement of animals in safety assessment. The objective of this study was to identify the animal-derived products that are used as part of in vitro methods for skin sensitisation testing. Thus, a systematic review of 156 articles featuring 83 different in vitro methods was carried out and, from this review, the use of several animal-derived products from different species was identified, with the use of fetal bovine serum being cited in most of the methods (78%). The use of sera from other animals, monoclonal antibodies and animal proteins were also variously mentioned. While non-animal alternatives are available and methods free of animal-derived products are emerging, most of the current methods reported used at least one animal-derived product, which raises ethical and technical concerns. Therefore, to deliver technically and ethically better in vitro methods for the safety assessment of chemicals, more effort should be made to replace products of animal origin in existing methods and to avoid their use in the development of new method protocols.

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