Determination of Diffusion Coefficients of Humic Substances by Fluorescence Correlation Spectroscopy:  Role of Solution Conditions

2000 ◽  
Vol 34 (7) ◽  
pp. 1365-1369 ◽  
Author(s):  
Jamie R. Lead ◽  
Kevin J. Wilkinson ◽  
Konstantin Starchev ◽  
Silvio Canonica ◽  
Jacques Buffle
Keyword(s):  
Humic Substances ◽  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation

Role of the tracer in characterizing the aggregation behavior of aqueous block copolymer solutions using fluorescence correlation spectroscopy

2006 ◽  
Vol 285 (5) ◽  
pp. 491-497 ◽  
Author(s):  
Tune B. Bonné ◽  
Christine M. Papadakis ◽  
Karin Lüdtke ◽  
Rainer Jordan
Keyword(s):  
Block Copolymer ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Aggregation Behavior ◽  
Fluorescence Correlation

Monte Carlo simulation of fluorescence correlation spectroscopy data

2011 ◽  
Vol 76 (3) ◽  
pp. 207-222 ◽  
Author(s):  
Peter Košovan ◽  
Filip Uhlík ◽  
Jitka Kuldová ◽  
Miroslav Štěpánek ◽  
Zuzana Limpouchová ◽  
...  
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Rotational Diffusion ◽  
Correlation Spectroscopy ◽  
Analytical Models ◽  
Spherical Particles ◽  
Fluorescence Signal ◽  
Fluorescence Correlation

We employed the Monte Carlo simulation methodology to emulate the diffusion of fluorescently labeled particles and understand the source of differences between values of diffusion coefficients (and consequently hydrodynamic radii) of fluorescently labeled nanoparticles measured by fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS). We used the simulation program developed in our laboratory and studied the diffusion of spherical particles of different sizes, which are labeled on their surface. In this study, we focused on two complicating effects: (i) multiple labeling and (ii) rotational diffusion which affect the fluorescence signal from large particles and hinder the analysis of autocorrelation functions according to simple analytical models. We have shown that the fluorescence fluctuations can be well fitted using the analytical model for small point-like particles, but the obtained parameters deviate in some cases significantly from the real ones. It means that the current data treatment yields apparent values of diffusion coefficients and other parameters only and the interpretation of experimental results for systems of particles with sizes comparable to the size of the active illuminated volume requires great care and precaution.

Accurate Diffusion Coefficients of Organosoluble Reference Dyes in Organic Media Measured by Dual-Focus Fluorescence Correlation Spectroscopy

ACS Nano ◽  
2015 ◽  
Vol 9 (7) ◽  
pp. 7360-7373 ◽  
Author(s):  
Karel Goossens ◽  
Mira Prior ◽  
Victor Pacheco ◽  
Dieter Willbold ◽  
Klaus Müllen ◽  
...  
Keyword(s):  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Organic Media ◽  
Fluorescence Correlation

Size determination of quantum dots with fluorescence correlation spectroscopy

2011 ◽  
Author(s):  
D. Hill ◽  
C. Ast ◽  
H.-G. Löhmannsröben ◽  
A. Zulqurnain ◽  
W. Parak ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Size Determination ◽  
Fluorescence Correlation

Determination of quantum yields of semiconductor nanocrystals at the single emitter level via fluorescence correlation spectroscopy

Nanoscale ◽  
2018 ◽  
Vol 10 (15) ◽  
pp. 7147-7154 ◽  
Author(s):  
Gerardo Abbandonato ◽  
Katrin Hoffmann ◽  
Ute Resch-Genger
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Semiconductor Nanocrystals ◽  
Correlation Spectroscopy ◽  
Quantum Yields ◽  
Fluorescence Correlation ◽  
Fluorescence Quantum Yields ◽  
Ultralow Concentration ◽  
Single Emitter

A microscopy-based method to determine fluorescence quantum yields ΦF of dispersed semiconductor nanocrystals at ultralow concentration with fluorescence correlation spectroscopy (FCS) is presented.

scholarly journals Variations in Plasma Membrane Topography Can Explain Heterogenous Diffusion Coefficients Obtained by Fluorescence Correlation Spectroscopy

2020 ◽  
Vol 8 ◽  
Author(s):  
Astrid Gesper ◽  
Stefan Wennmalm ◽  
Philipp Hagemann ◽  
Sven-Göran Eriksson ◽  
Patrick Happel ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Diffusion Coefficients ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Membrane Topography
Sign in / Sign up

Export Citation Format

Share Document