Mechanistic insight into the reaction of phenyl isocyanate with ditungsten hexa-tert-butoxide: isolation and characterization of early intermediates

1985 ◽  
Vol 107 (16) ◽  
pp. 4662-4667 ◽  
Author(s):  
F. Albert Cotton ◽  
Edwar S. Shamshoum
Keyword(s):  
Phenyl Isocyanate ◽  
Isolation And Characterization ◽  
Mechanistic Insight ◽  
Insight Into

scholarly journals Substrate-dependent competition and cooperation relationships between Geobacter and Dehalococcoides for their organohalide respiration

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Yongyi Liang ◽  
Qihong Lu ◽  
Zhiwei Liang ◽  
Xiaokun Liu ◽  
Wenwen Fang ◽  
...  
Keyword(s):  
Electron Donors ◽  
Community Stability ◽  
Organohalide Respiration ◽  
Competition And Cooperation ◽  
Free Competition ◽  
Isolation And Characterization ◽  
Geochemical Cycling ◽  
New Strategy ◽  
Insight Into

AbstractObligate and non-obligate organohalide-respiring bacteria (OHRB) play central roles in the geochemical cycling and environmental bioremediation of organohalides. Their coexistence and interactions may provide functional redundancy and community stability to assure organohalide respiration efficiency but, at the same time, complicate isolation and characterization of specific OHRB. Here, we employed a growth rate/yield tradeoff strategy to enrich and isolate a rare non-obligate tetrachloroethene (PCE)-respiring Geobacter from a Dehalococcoides-predominant microcosm, providing experimental evidence for the rate/yield tradeoff theory in population selection. Surprisingly, further physiological and genomic characterizations, together with co-culture experiments, revealed three unique interactions (i.e., free competition, conditional competition and syntrophic cooperation) between Geobacter and Dehalococcoides for their respiration of PCE and polychlorinated biphenyls (PCBs), depending on both the feeding electron donors (acetate/H2 vs. propionate) and electron acceptors (PCE vs. PCBs). This study provides the first insight into substrate-dependent interactions between obligate and non-obligate OHRB, as well as a new strategy to isolate fastidious microorganisms, for better understanding of the geochemical cycling and bioremediation of organohalides.

scholarly journals Discovery and characterization of Hv1-type proton channels in reef-building corals.

2021 ◽  
Author(s):  
Gisela Rangel-Tescas ◽  
Cecilia Cervantes ◽  
Miguel A Cervantes-Rocha ◽  
Esteban Suarez-Delgado ◽  
Anastazia T Banaszak ◽  
...  
Keyword(s):  
Voltage Dependence ◽  
Ph Regulation ◽  
Functional Characterization ◽  
Gene Encoding ◽  
Proton Channels ◽  
Gating Model ◽  
Mechanistic Insight ◽  
Voltage Dependent ◽  
Insight Into

Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding for Hv1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a manner that makes them interesting models for studying these processes more easily. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH regulation processes and possible consequences of ocean acidification related to the function of these channels.

scholarly journals Formation and characterization of a reactive chromium(v)–oxo complex: mechanistic insight into hydrogen-atom transfer reactions

2015 ◽  
Vol 6 (2) ◽  
pp. 945-955 ◽  
Author(s):  
Hiroaki Kotani ◽  
Suzue Kaida ◽  
Tomoya Ishizuka ◽  
Miyuki Sakaguchi ◽  
Takashi Ogura ◽  
...  
Keyword(s):  
Hydrogen Atom ◽  
Reaction Mechanisms ◽  
Hydrogen Atom Transfer ◽  
Transfer Reactions ◽  
Atom Transfer ◽  
Oxidation Potentials ◽  
Mechanistic Insight ◽  
Atom Transfer Reactions ◽  
Insight Into

Mechanistic insights were gained into hydrogen-atom transfer reactions from benzyl alcohol derivatives with different oxidation potentials to a highly reactive Cr(v)–oxo complex to reveal switching of reaction mechanisms.

scholarly journals 210 Mechanistic insight into the repigmentation of piebaldism: Functional characterization of a mutant KIT in melanocyte regeneration

2017 ◽  
Vol 137 (10) ◽  
pp. S228
Author(s):  
A. Shimizu ◽  
M. Hattori ◽  
D. Oikawa ◽  
H. Amano ◽  
A. Ishida-Yamamoto ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Mechanistic Insight ◽  
Insight Into

Nickel-catalyzed reductive defunctionalization of esters and amides to aromatic hydrocarbons

2017 ◽  
Vol 41 (22) ◽  
pp. 13211-13214 ◽  
Author(s):  
Manoj Mondal ◽  
Pankaj Bharali
Keyword(s):  
Aromatic Hydrocarbons ◽  
Bond Activation ◽  
Bond Cleavage ◽  
Reductive Activation ◽  
One Pot ◽  
Activation Pathway ◽  
Isolation And Characterization ◽  
Insight Into

In two recent studies, nickel has been used in the removal of ester and amide groups to afford aromatic hydrocarbons via one-pot reductive activation followed by tandem C–CO bond cleavage–decarbonylation. Isolation and characterization of key intermediates provide insight into this acyl C–O bond activation pathway.

scholarly journals Isolation and characterization of extracellular vesicles from Broncho-alveolar lavage fluid: a review and comparison of different methods

2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Jonathan M. Carnino ◽  
Heedoo Lee ◽  
Yang Jin
Keyword(s):  
Extracellular Vesicles ◽  
Culture Medium ◽  
Bronchoalveolar Lavage Fluid ◽  
Cell Communication ◽  
Lavage Fluid ◽  
Surface Marker ◽  
Cell Culture Medium ◽  
Isolation And Characterization ◽  
Insight Into

Abstract Extracellular vesicles (EVs) are cell-derived membranous vesicles secreted by cells into the extracellular space, which play a role in cell to cell communication. EVs are categorized into 3 groups depending on their size, surface marker, and method of release from the host cell. Recently, EVs have become of interest in the study of multiple disease etiologies and are believed to be potential biomarkers for many diseases. Multiple different methods have been developed to isolate EVs from different samples such as cell culture medium, serum, blood, and urine. Once isolated, EVs can be characterized by technology such as nanotracking analysis, dynamic light scattering, and nanoscale flow cytometry. In this review, we summarize the current methods of EV isolation, provide details into the three methods of EV characterization, and provide insight into which isolation approaches are most suitable for EV isolation from bronchoalveolar lavage fluid (BALF).

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