scholarly journals Discovery and characterization of Hv1-type proton channels in reef-building corals.

2021 ◽  
Author(s):  
Gisela Rangel-Tescas ◽  
Cecilia Cervantes ◽  
Miguel A Cervantes-Rocha ◽  
Esteban Suarez-Delgado ◽  
Anastazia T Banaszak ◽  
...  
Keyword(s):  
Voltage Dependence ◽  
Ph Regulation ◽  
Functional Characterization ◽  
Gene Encoding ◽  
Proton Channels ◽  
Gating Model ◽  
Mechanistic Insight ◽  
Voltage Dependent ◽  
Insight Into

Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding for Hv1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a manner that makes them interesting models for studying these processes more easily. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH regulation processes and possible consequences of ocean acidification related to the function of these channels.

scholarly journals Discovery and characterization of Hv1-type proton channels in reef-building corals

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Gisela Rangel-Yescas ◽  
Cecilia Cervantes ◽  
Miguel A Cervantes-Rocha ◽  
Esteban Suárez-Delgado ◽  
Anastazia T Banaszak ◽  
...  
Keyword(s):  
Ph Regulation ◽  
Functional Characterization ◽  
Model Systems ◽  
Biophysical Properties ◽  
Gene Encoding ◽  
Proton Channels ◽  
Gating Model ◽  
Voltage Dependent ◽  
Insight Into

Voltage-dependent proton-permeable channels are membrane proteins mediating a number of important physiological functions. Here we report the presence of a gene encoding Hv1 voltage-dependent, proton-permeable channels in two species of reef-building corals. We performed a characterization of their biophysical properties and found that these channels are fast-activating and modulated by the pH gradient in a distinct manner. The biophysical properties of these novel channels make them interesting model systems. We have also developed an allosteric gating model that provides mechanistic insight into the modulation of voltage-dependence by protons. This work also represents the first functional characterization of any ion channel in scleractinian corals. We discuss the implications of the presence of these channels in the membranes of coral cells in the calcification and pH-regulation processes and possible consequences of ocean acidification related to the function of these channels.

scholarly journals 210 Mechanistic insight into the repigmentation of piebaldism: Functional characterization of a mutant KIT in melanocyte regeneration

2017 ◽  
Vol 137 (10) ◽  
pp. S228
Author(s):  
A. Shimizu ◽  
M. Hattori ◽  
D. Oikawa ◽  
H. Amano ◽  
A. Ishida-Yamamoto ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Mechanistic Insight ◽  
Insight Into

Identification and functional characterization of levS, a gene encoding for a levansucrase from Leuconostoc mesenteroides NRRL B-512 F

Gene ◽  
2006 ◽  
Vol 376 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Sandra Morales-Arrieta ◽  
Maria Elena Rodríguez ◽  
Lorenzo Segovia ◽  
Agustín López-Munguía ◽  
Clarita Olvera-Carranza
Keyword(s):  
Leuconostoc Mesenteroides ◽  
Functional Characterization ◽  
Gene Encoding

Characterization of voltage-dependent calcium currents in mouse motoneurons

1992 ◽  
Vol 68 (1) ◽  
pp. 85-92 ◽  
Author(s):  
M. Mynlieff ◽  
K. G. Beam
Keyword(s):  
Voltage Dependence ◽  
Low Voltage ◽  
Calcium Currents ◽  
Patch Clamp Technique ◽  
Maximal Amplitude ◽  
Time To Peak ◽  
Whole Cell Patch Clamp ◽  
Voltage Dependent ◽  
Channel Currents

1. Calcium channel currents were measured with the whole-cell patch clamp technique in cultured, identified mouse motoneurons. Three components of current were operationally defined on the basis of voltage dependence, kinetics, and pharmacology. 2. Test potentials to -50 mV or greater (10 mM external Ca2+) elicited a low-voltage activated T-type current that was transient (decaying to baseline in less than 200 ms) and had a relatively slow time to peak (20-50 ms). A 1-s prepulse to -45 mV produced approximately half-maximal inactivation of this T current. 3. Two high-voltage activated (HVA) components of current (1 transient and 1 sustained) were activated by test potentials to -20 mV or greater (10 mM external Ca2+). A 1-s prepulse to -35 mV produced approximately half-maximal inactivation of the transient component without affecting the sustained component. 4. When Ba2+ was substituted for Ca2+ as the charge carrier, activation of the HVA components was shifted in the hyperpolarizing direction, and the relative amplitude of the transient HVA component was reduced. 5. Amiloride (1-2 mM) caused a reversible, partial block of the T current without affecting the HVA components. 6. The dihydropyridine agonist isopropyl 4-(2,1,3-benzoxadiazol-4-yl)-1,4-dihydro-2,6-dimethyl-5-nitro-3- pyridine-carboxylate [(+)-SDZ 202-791, 100 nM-1 microM)] shifted the activation of the sustained component of HVA current to more negative potentials and increased its maximal amplitude. Additionally, (+)-SDZ 202-791 caused the appearance of a slowed component of tail current.(ABSTRACT TRUNCATED AT 250 WORDS)

Functional characterization of the Haemophilus influenzae 4.5S RNA

1998 ◽  
Vol 44 (1) ◽  
pp. 91-94
Author(s):  
G Scott Jenkins ◽  
Mark S Chandler ◽  
Pamela S Fink
Keyword(s):  
Haemophilus Influenzae ◽  
Functional Characterization ◽  
Coli Strain ◽  
Northern Analysis ◽  
Gene Encoding ◽  
E Coli ◽  
Functional Homolog ◽  
Polymerase Chain ◽  
4.5S Rna

The putative 4.5S RNA of Haemophilus influenzae was identified in the genome by computer analysis, amplified by the polymerase chain reaction, and cloned. We have determined that this putative 4.5S RNA will complement an Escherichia coli strain conditionally defective in 4.5S RNA production. The predicted secondary structures of the molecules were quite similar, but Northern analysis showed that the H. influenzae RNA was slightly larger than the E. coli RNA. The H. influenzae gene encoding this RNA is the functional homolog of the ffs gene in E. coli. Key words: ffs gene, complementation studies, small RNA, prokaryotic genetics.

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