Post-Column Denaturation-Assisted Native Size-Exclusion Chromatography–Mass Spectrometry for Rapid and In-Depth Characterization of High Molecular Weight Variants in Therapeutic Monoclonal Antibodies

2021 ◽  
Author(s):  
Yuetian Yan ◽  
Tao Xing ◽  
Anita P. Liu ◽  
Zhengqi Zhang ◽  
Shunhai Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Monoclonal Antibodies ◽  
High Molecular Weight ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Chromatography Mass Spectrometry ◽  
Exclusion Chromatography ◽  
Therapeutic Monoclonal Antibodies

scholarly journals Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates

2017 ◽  
Vol 8 (5) ◽  
pp. 4062-4072 ◽  
Author(s):  
Michael Cammarata ◽  
Ross Thyer ◽  
Michael Lombardo ◽  
Amy Anderson ◽  
Dennis Wright ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Dihydrofolate Reductase ◽  
Native Mass Spectrometry ◽  
Size Exclusion ◽  
E Coli ◽  
Exclusion Chromatography

Native mass spectrometry, size exclusion chromatography, and kinetic assays were employed to study trimethoprim resistance in E. coli caused by mutations P21L and W30R of dihydrofolate reductase.

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