Approach to the profiling and characterization of influenza vaccine constituents by the combined use of size-exclusion chromatography, gel electrophoresis and mass spectrometry

Biologicals ◽  
2010 ◽  
Vol 38 (2) ◽  
pp. 294-302 ◽  
Author(s):  
Virginia Garcia-Cañas ◽  
Barry Lorbetskie ◽  
Terry D. Cyr ◽  
Mary A. Hefford ◽  
Sophie Smith ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Influenza Vaccine ◽  
Size Exclusion Chromatography ◽  
Gel Electrophoresis ◽  
Size Exclusion ◽  
Combined Use ◽  
Exclusion Chromatography

scholarly journals Characterization of trimethoprim resistant E. coli dihydrofolate reductase mutants by mass spectrometry and inhibition by propargyl-linked antifolates

2017 ◽  
Vol 8 (5) ◽  
pp. 4062-4072 ◽  
Author(s):  
Michael Cammarata ◽  
Ross Thyer ◽  
Michael Lombardo ◽  
Amy Anderson ◽  
Dennis Wright ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Size Exclusion Chromatography ◽  
Dihydrofolate Reductase ◽  
Native Mass Spectrometry ◽  
Size Exclusion ◽  
E Coli ◽  
Exclusion Chromatography

Native mass spectrometry, size exclusion chromatography, and kinetic assays were employed to study trimethoprim resistance in E. coli caused by mutations P21L and W30R of dihydrofolate reductase.

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