scholarly journals Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

2020 ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Nancy M. El-Shweihy
Keyword(s):  
Yeast Extract ◽  
Cholesterol Oxidase ◽  
Agitation Speed ◽  
Isolation And Identification ◽  
Box Behnken Design ◽  
Positive Effects ◽  
Wide Range ◽  
Burman Design ◽  
Promising Source ◽  
Plackett Burman Design

Abstract Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.

scholarly journals Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

2020 ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Nancy M. El-Shweihy
Keyword(s):  
Yeast Extract ◽  
Cholesterol Oxidase ◽  
Agitation Speed ◽  
Isolation And Identification ◽  
Box Behnken Design ◽  
Positive Effects ◽  
Wide Range ◽  
Burman Design ◽  
Promising Source ◽  
Plackett Burman Design

Abstract Background: Cholesterol oxidase biosensors have been used to determine the level of cholesterol in different serum and food samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and identification of a new microbial source (s) of cholesterol oxidase are very important. Results: The local isolate Streptomyces sp. strain NEAE-94 is a promising source of cholesterol oxidase. It was identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence. The sequencing product had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 in shake flasks was optimized using surface response methodology. The different process parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentrations had the most significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. Cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 was 11.03, 27.31 U/mL after Plackett-Burman Design and Box-Behnken design; respectively, with a fold of increase of 6.06 times compared to the production before applying the Plackett-Burman design (4.51 U/mL). Conclusions: Maximum cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4 % (v/v), temperature 37°C, agitation speed 150 rpm, medium volume 50 mL and incubation time 5 days.

scholarly journals Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94

2019 ◽  
Author(s):  
Noura El-Ahmady El-Naggar ◽  
Nancy M. El-Shweihy
Keyword(s):  
Yeast Extract ◽  
Oxidase Activity ◽  
Cholesterol Oxidase ◽  
Agitation Speed ◽  
Cholesterol Concentration ◽  
Positive Effects ◽  
Extract Concentration ◽  
Wide Range ◽  
Burman Design ◽  
Plackett Burman Design

Abstract Background Cholesterol oxidase biosensors have been applied to detect cholesterol level in different in serum and foods samples. Due to a wide range of industrial and clinical applications of microbial cholesterol oxidase, isolation and detection of new microbial source (s) of cholesterol oxidase are very important.Results Among the potential strains, Streptomyces sp. strain NEAE-94 was chosen and identified based on cultural, morphological and physiological characteristics; in addition to the 16S rRNA sequence which had been deposited in the GenBank database under the accession number KC354803. Cholesterol oxidase production in shake flask by Streptomyces anulatus strain NEAE-94 was optimized using response surface methodology. The production parameters were first screened using a Plackett-Burman design and the parameters with significant effects on the production of cholesterol oxidase were identified. Out of the fifteen factors screened, agitation speed, cholesterol and yeast extract concentration were selected due to significant positive effects on the production of cholesterol oxidase. The optimal levels of these variables and the effects of their mutual interactions on cholesterol oxidase production were determined using Box-Behnken design. The maximum cholesterol oxidase activity (27.31 U/mL) was achieved at cholesterol concentration (4 g/L), the agitation speed (150 rpm/min) and yeast extract concentration (5 g/L). In comparison with cholesterol oxidase production by Streptomyces anulatus strain NEAE-94 before the application of Plackett-Burman design, the statistical optimization resulted in an increased production by 4.66 times.Conclusions The maximal cholesterol oxidase activity is obtained at the following fermentation conditions: g/L (cholesterol 4, yeast extract 5, NaCl 0.5, K 2 HPO 4 1, FeSO 4 .7H 2 O 0.01, MgSO 4 .7H 2 O 0.5), pH 7, inoculum size 4% (v/v), temperature 37°C, agitation speed 150 rpm/min, medium volume 100 mL and incubation time 5 days.

DESIGN OF CULTURAL CONDITIONS FOR PRODUCTION OF CHONDROITINASE BY ASPERGILLUS NIGER USING DESIGN OF EXPERIMENTS

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (06) ◽  
pp. 24-32
Author(s):  
N Kasinathan ◽  
◽  
V. M. Subrahmanyam ◽  
V. R Josyula
Keyword(s):  
Design Of Experiments ◽  
Yeast Extract ◽  
Spinal Cord Injuries ◽  
Box Behnken Design ◽  
Medium Components ◽  
Cultural Conditions ◽  
Burman Design ◽  
Level Of Significance ◽  
Optimized Conditions ◽  
Plackett Burman Design

Chondroitinases are useful in treating cancer and spinal cord injuries. The work reported so far does not focus on production of fungal chondroitinase. The present study focuses on the optimization of cultural conditions using design of experiments for production of chondroitinase by a fungal isolate CHCS-2, obtained from humus containing poultry waste. The cultural conditions were optimized systematically using a one-factor-at-a-time (OFAT) approach, factorial design, Plackett-Burman design (PBD) and Box- Behnken design to identify the composition of the production medium and the level of the variables. The level of significance of the medium components selected by OFAT study viz., yeast extract, chondroitin, and phosphate, was analyzed by factorial studies. The effect of eleven minerals on the enzyme yield was studied by PBD. The interaction among the medium components selected using factorial and PBD viz., yeast extract, cobalt and aluminum was investigated using the Box-Behnken design (BBD). Under the optimized conditions, maximum chondroitinase yield was 22.5 U/mL.

Enhancement of Hydrogen Production from Sugarcane Bagasse by Klebsiella pneumoniae subsp. pneumonia DSM 30104(T) Using Statistical Methods

2017 ◽  
Vol 757 ◽  
pp. 146-150
Author(s):  
Piyawadee Saraphirom ◽  
Mullika Teerakul ◽  
Manit Anyabho
Keyword(s):  
Potassium Chloride ◽  
Optimal Condition ◽  
Calcium Chloride ◽  
Sugarcane Bagasse ◽  
Yeast Extract ◽  
Total Sugar ◽  
Sugarcane Bagasse Hydrolysate ◽  
Box Behnken Design ◽  
Burman Design ◽  
Plackett Burman Design

This study employed statistically based experimental designs to optimize environmental factors for H2 production from sugarcane bagasse hydrolysate by K. pneumoniae subsp. Pneumonia DSM 30104(T) isolated from wastewater sludge using statistical method. The 12 runs of Plackett-Burman design were used to classify important factors influencing the H2 production from sugarcane bagasse hydrolysate by K. pneumonia subsp. pneumonia DSM 30104(T). Mutual interaction between the significant factor and their optimal values that brought the maximum H2 production (mL H2/L) were further investigated using Box-Behnken design of response surface method. Experimental results indicated that yeast extract, ammonium chloride, potassium chloride, calcium chloride, iron (II) sulfate and total sugar of sugarcane bagasse had an interdependent effect on the maximum H2 production while only interaction effect between yeast extract and calcium chloride had statistically significant (P≤0.05) influences on the maximum H2 production. Optimal conditions for the predicted maximal H2 production were 7.50 g/L yeast extract, 0.50 g/L ammonium chloride, 15.0 g/L potassium chloride, 0.75 g/L calcium chloride, and 10.0 g/L total sugar. At the optimal condition, the maximum H2 production of 277 mL H2/L was estimated from Box-Behnken design that more than 9 times compared to Plackett-Burman design. The highest ratio of butyric acid to acetic acid (B/A ratio) of 1.47 was indicated the high performance of H2 fermentation of sugarcane bagasse hydrolysate by K. pneumoniae subsp. Pneumonia DSM 30104(T) under the optimal condition obtained.

Plackett-Burman Design for Media Nutrients of Biocontrol Lysinibacillus xylanilyticus BPM1 against Aflatoxin

2014 ◽  
Vol 522-524 ◽  
pp. 295-298
Author(s):  
Kai Wang ◽  
Pei Sheng Yan ◽  
Li Xin Cao
Keyword(s):  
Biological Control ◽  
Yeast Extract ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Peanut Hulls ◽  
Two Factors ◽  
Burman Design ◽  
Soya Peptone ◽  
Plackett Burman Design ◽  
Biocontrol Bacterium

Aflatoxins (AFs) are a series of highly toxic and carcinogenic secondary metabolites. In order to eliminate AFs contamination, biological control is one of the more promising techniques. In this study, we describe the optimization of media nutrients for the selected biocontrol bacterium, Lysinibacillus xylanilyticus strain BPM1. The strain was isolated from the peanut hulls in Shandong Province, China and exhibited antagonistic activity against aflatoxins. Maltose and sucrose were identified as best carbon source, while soya peptone and yeast extract as nitrogen source led to the highest OD600 observations. Medium composition was optimized using Plackett-Burman design, which was applied to find the key ingredients. The results revealed that the most significant two factors which were more effective in the fermentation of L. xylanilyticus BPM1 were soya peptone and yeast extract.

scholarly journals Engineering rice based medium for production of lovastatin with Monascus species

2009 ◽  
Vol 27 (No. 5) ◽  
pp. 352-360 ◽  
Author(s):  
B.P. Panda ◽  
S. Javed ◽  
M. Ali
Keyword(s):  
Blood Lipid ◽  
Red Yeast Rice ◽  
Red Rice ◽  
Box Behnken Design ◽  
Glutinous Rice ◽  
Lower Blood ◽  
Red Mold Rice ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Blood Lipid Levels

Angkak (red mold rice, red yeast rice, Chinese red rice), a traditional Chinese functional food is produced by solid-state fermentation of cooked non-glutinous rice with <i>Monascus</i> species. The secondary metabolite of <i>Monascus</i> species, monacolin K (lovastatin), has been proved to lower blood lipid levels. In this study, the best <i>Monascus purpureus</i> strain was selected from MTCC 369, 410, 1090 based on lovastatin concentration. Four medium parameters (NH<sub>4</sub>Cl, MgSO<sub>4</sub>, NaCl, CaCl<sub>2</sub>) screened by Plackett-Burman design from total nine medium variables were optimised by Box-Behnken design of response surface methodology. Maximum lovastatin production of 3.420 mg/g was predicted in the solid medium containing 20 g rice and 40 ml liquid nutrient (NH<sub>4</sub>Cl 14.32 g/l, MgSO<sub>4</sub> 0.76 g/l, NaCl 14.65 g/l, and CaCl<sub>2</sub> 0.54 g/l) by the point prediction tool of Design Expert Ver. 7.1 software at 14<sup>th</sup> day of fermentation.

Enhanced Production of Mycelia by the Medicinal Mushroom Cordyceps militaris Using Plackett-Burman Design and Response Surface Methodology

2011 ◽  
Vol 138-139 ◽  
pp. 1209-1214
Author(s):  
Xiao Yu Liu ◽  
Fan Xing Meng ◽  
Yi Bo Zhang ◽  
Huan He ◽  
Wei Han ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Yeast Extract ◽  
Dry Weight ◽  
Fermentation Medium ◽  
Cordyceps Militaris ◽  
Enhanced Production ◽  
Burman Design ◽  
Optimization Of Fermentation ◽  
Plackett Burman Design

Response surface methodology (RSM) was used for statistical optimization of fermentation medium that influenced the yield of endo-polysaccharide from cultivated mycelia of Cordyceps militaris. First, the Plackett-Burman design was used to evaluate the effects of ten variables including glucose, maltose, peptone, yeast extract, KH2PO4, MgSO4, CaCl2, VB1, inoculum density and medium capacity. Among these variables, glucose, peptone and yeast extract were identified to have the significant effects. Subsequently, response surface methodology based on a five-level three-factor central composite design was employed to determine the maximum dry weight (DW) of mycelial biomass at optimum concentration of glucose, peptone and yeast extract. The mycelia growth was found to correlate to the three parameters that could be represented by second-order polynomial models. The optimal values of the three parameters were determined as 4.62% glucose, 3.36% peptone and 0.43% yeast extract. The prediction DW was 23.727g/L. The actual experimental results were in agreement with the prediction.

scholarly journals Fungal Endophytes of Vitis vinifera—Plant Growth Promotion Factors

Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1250
Author(s):  
Markéta Kulišová ◽  
Maria Vrublevskaya ◽  
Petra Lovecká ◽  
Blanka Vrchotová ◽  
Milena Stránská ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Endophytic Fungi ◽  
Growth Promotion ◽  
Fungal Endophytes ◽  
Plant Tissues ◽  
Antifungal Compounds ◽  
Microscopic Fungi ◽  
Isolation And Identification ◽  
Wide Range ◽  
Promising Source

Endophytes are microorganisms that live asymptomatically inside plant tissues. They are beneficial to their host in many aspects, especially as a defense against foreign phytopathogens through the production of a variety of metabolites. These substances can serve as sources of new natural products for medicinal, agricultural, and industrial purposes. This article is focused on endophytic fungi from Vitis vinifera. The purpose of the research was their isolation and identification during the Vitis vinifera growing season. Subsequently, the isolates were tested for the production of biotechnologically interesting metabolites (siderophores, antioxidants, and antifungal compounds). In total, 24 endophytic fungi were isolated, the most represented genus was Cladosporium sp. The results of the test for antioxidant and antifungal properties, as well as siderophore production, have shown that the population of Vitis vinifera endophytic microscopic fungi could serve as a promising source of metabolites with a wide range of applications.

scholarly journals Optimization of Fungal Dextranase Production and Its Antibiofilm Activity, Encapsulation and Stability in Toothpaste

Molecules ◽  
2020 ◽  
Vol 25 (20) ◽  
pp. 4784 ◽  
Author(s):  
Nucharee Juntarachot ◽  
Duangporn Kantachote ◽  
Sartjin Peerajan ◽  
Sasithorn Sirilun ◽  
Chaiyavat Chaiyasut
Keyword(s):  
Dental Caries ◽  
Yeast Extract ◽  
Suitable Condition ◽  
Alginate Beads ◽  
Antibiofilm Activity ◽  
Inoculum Age ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Plaque Biofilm ◽  
Significant Factors

Dextranase catalyzes the degradation of the substrate dextran, which is a component of plaque biofilm. This enzyme is involved in antiplaque accumulation, which can prevent dental caries. The activity of crude dextranase from Penicillium roquefortii TISTR 3511 was assessed, and the maximum value (7.61 unit/g) was obtained at 37 °C and pH 6. The Plackett–Burman design was used to obtain significant factors for enhancing fungal dextranase production, and three influencing factors were found: Dextran, yeast extract concentration and inoculum age. Subsequently, the significant factors were optimized with the Box–Behnken design, and the most suitable condition for dextranase activity at 30.24 unit/g was achieved with 80 g/L dextran, 30 g/L yeast extract and five day- old inoculum. The use of 0.85% alginate beads for encapsulation exhibited maximum dextranase activity at 25.18 unit/g beads, and this activity was stable in toothpaste for three months of testing. This study explored the potential production of fungal dextranase under optimal conditions and its encapsulation using alginate for the possibility of applying encapsulated dextranase as an additive in toothpaste products for preventing dental caries.

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