Assessment of Enzymatic Methods in the δ18O Value Determination of thel-Tyrosinep-Hydroxy Group for Proof of Illegal Meat and Bone Meal Feeding to Cattle

2011 ◽  
Vol 59 (17) ◽  
pp. 9475-9483 ◽  
Author(s):  
Nicole Tanz ◽  
Roland A. Werner ◽  
Wolfgang Eisenreich ◽  
Hanns-Ludwig Schmidt

Author(s):  
P.B. Lynch ◽  
P.J.A. Sheehy

Dietary supplementation with folic acid has been shown to improve reproductive performance in sows (Lindemann 1993). However most studies have been for one cycle only and few have examined the effect of supplementation over several parities.One hundred and thirty four crossbred sows ranging in parity from 2 to 4 were selected at farrowing and randomly allocated to two dietary treatments of low and high supplemental folic acid (0 and 10 g per tonne, Roche Products Ltd.). Treatments were applied for the following three lactations and post weaning periods, two full pregnancies and to day 30 of the pregnancy following the third lactation. The diet fed contained barley, wheat, soyabean meal and meat and bone meal with nutrient levels of 14.0 MJ DE/kg and 1.02% lysine. Sows were individually penned throughout with restricted feeding in pregnancy (2.2 kg/day increasing to 2.5 kg/day in the final month), and ad libitum in lactation (approx 5.0 kg/day) and post weaning (approx 3.4 kg/day). Blood samples for determination of plasma and red cell folate were taken from 14 sows per treatment on days 4, 50 and 110 of one cycle. These were determined by a microbiological assay (modification of methods of Scott et al 1974 and Wilson and Home 1982).



The Analyst ◽  
1998 ◽  
Vol 123 (12) ◽  
pp. 2513-2516 ◽  
Author(s):  
Dick Hooijerink ◽  
Robert Schilt ◽  
Bert Brouwer ◽  
Eric van Bennekom


2014 ◽  
Vol 32 (5) ◽  
pp. 423-433 ◽  
Author(s):  
Małgorzata Wzorek

The objective of this article is to elaborate a method to optimize the composition of the fuels from sewage sludge (PBS fuel – fuel based on sewage sludge and coal slime, PBM fuel – fuel based on sewage sludge and meat and bone meal, PBT fuel – fuel based on sewage sludge and sawdust). As a tool for an optimization procedure, the use of a genetic algorithm is proposed. The optimization task involves the maximization of mass fraction of sewage sludge in a fuel developed on the basis of quality-based criteria for the use as an alternative fuel used by the cement industry. The selection criteria of fuels composition concerned such parameters as: calorific value, content of chlorine, sulphur and heavy metals. Mathematical descriptions of fuel compositions and general forms of the genetic algorithm, as well as the obtained optimization results are presented. The results of this study indicate that the proposed genetic algorithm offers an optimization tool, which could be useful in the determination of the composition of fuels that are produced from waste.



1958 ◽  
Vol 41 (2) ◽  
pp. 411-413
Author(s):  
Milton Lapidus ◽  
Edward F Mellon
Keyword(s):  


2019 ◽  
Vol 36 (3) ◽  
pp. 424-433 ◽  
Author(s):  
Carolina N. Aroeira ◽  
Vivian Feddern ◽  
Vanessa Gressler ◽  
Luciano Molognoni ◽  
Heitor Daguer ◽  
...  
Keyword(s):  


2004 ◽  
Vol 87 (6) ◽  
pp. 1334-1341 ◽  
Author(s):  
Giséile Gizzi ◽  
Christoph von Holst ◽  
Vincent Baeten ◽  
Gilbert Berben ◽  
Leo van Raamsdonk

Abstract An intercomparison study was conducted to determine the presence of processed animal proteins (PAPs), including meat and bone meal (MBM) from various species, in animal feed. The performances of different methods, such as microscopy, polymerase chain reaction (PCR), immunoassays, and a protocol based on iquid chromatography (LC), were compared. Laboratories were asked to analyze for PAPs from all terrestrial animals and fish (total PAPs); mammalian PAPs; ruminant PAPs; and porcine PAPs. They were free to use their method of choice. In addition, laboratories using microscopy were asked to determine the presence of PAPs from terrestrial animals, which is applicable only to microscopy. For total PAPs microscopy, LC and some immunoassays showed sufficient results at a concentration as low as 0.1% MBM in the feed. In contrast, PCR was not fit for purpose. In differentiating between MBM from terrestrial animals and fishmeal, microscopy detected 0.5% of terrestrial MBM in feed in the presence of 5% fishmeal, but was less successful when the concentration of MBM from terrestrial animals was 0.1%. The animal-specific determination of MBM from mammals or, more specifically from either ruminants or pigs, by PCR showed poor results, as indicated by a high number of false-positive and false-negative results. The only PCR method that scored quite well was applied by a member of the organizer team of the study. Immunoassays scored much better than PCR, showing sufficient sensitivity but some deficiency in terms of specificity. The results also demonstrated that the reliable determination of MBM from ruminants has not been resolved, especially for low concentrations of MBM (0.1%) in feed. Comparison of the results for mammalian MBM from all methods indicated that, for control purposes, the immunoassay method, especially when applied as dipsticks, could be used as a rapid screening method combined with microscopy to confirm the positive samples. However, implementation of such a system would require that the immunoassays were previously validated to demonstrate that this approach is fit for purpose. The determination of ruminant or porcine PAPs by immunoassays was more difficult, partly because the MBM in this study contained about 50% bovine and porcine material, thereby reducing the target concentration level to 0.05%.



2016 ◽  
Vol 39 (7) ◽  
pp. 937-945
Author(s):  
Yi Soo Kim ◽  
Sung Joong Lee ◽  
Jin Young Choi ◽  
Yun-Hi Kim ◽  
Kebede Taye Desta ◽  
...  


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