Straightforward Process for Removal of Milk Fat Globule Membranes and Production of Fat-free Whey Protein Concentrate from Cheese Whey

2011 ◽  
Vol 59 (18) ◽  
pp. 10271-10276 ◽  
Author(s):  
Srinivasan Damodaran
Keyword(s):  
Whey Protein ◽  
Milk Fat ◽  
Cheese Whey ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes

scholarly journals Isolation and characterization of two individual glycoprotein components from human milk-fat-globule membranes

1982 ◽  
Vol 207 (1) ◽  
pp. 37-41 ◽  
Author(s):  
A Imam ◽  
D J Laurence ◽  
A M Neville
Keyword(s):  
Human Milk ◽  
Milk Fat ◽  
Sodium Dodecyl ◽  
Membrane Glycoproteins ◽  
Lectin Affinity Chromatography ◽  
Isolation And Characterization ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes ◽  
Human Milk Fat

Two individual glycoprotein components from human milk-fat-globule membranes (MFGM) has been purified by selectively extracting the membrane glycoproteins followed by lectin affinity chromatography and gel filtration on Sephadex G-200 in the presence of protein-disaggregating agents. The purified glycoprotein components, termed ‘epithelial-membrane glycoprotein’ (EMGP-155 and EMGP-39) have estimated molecular weights of 155 000 and 39 000 respectively, and yield a single band under reducing conditions on sodium dodecyl sulphate/polyacrylamide gel. EMGP-155 and EMGP-39 contain 21.0% and 7.0% carbohydrate by weight, with fucose (13.5%, 12.4%), mannose (3.7%, 6.2%), galactose (28.5%, 22.6%), N-acetylglucosamine (17.8%, 7.4%) and sialic acid (36.4%, 51.4%) of the carbohydrate moiety respectively. For both the glycoprotein components, aspartic and glutamic acid and serine are the major amino acid residues.

scholarly journals Mutu Keju Putih Rendah Lemak Diproduksi Dengan Bahan Baku Susu Modifikasi

2016 ◽  
Vol 40 (2) ◽  
pp. 144 ◽  
Author(s):  
Abubakar Abubakar
Keyword(s):  
Whey Protein ◽  
Milk Fat ◽  
Dry Matter ◽  
Corn Oil ◽  
Skim Milk ◽  
Raw Material ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Water Emulsion ◽  
White Cheese

This research was conducted to investigate the quality of low-fat white cheese produced using raw material of modified milk. Five treatments applied were (A1) Using reduced fat (60%) milk, (A2) Using emulsion of corn oil in skim milk (replacing milk fat with corn oil), (A3) Using emulsion of corn oil in skim milk and addition of whey protein concentrate (replacing milk fat with corn oil and the addition of whey protein concentrate=WPC), (A4) Using skim milk and water emulsion oil in water, and (A5) replacing milk fat with corn oil and the addition of probiotic (Lactobacillus casei). Each treatment was replicated three times. The selected that skim milk in corn oil emulsion with the addition of probiotics, the results showed had cheese quality characteristics as follow: yield 12.94±0.16%, hardnes 48.07±10.12 g, softness 8.51±0.54 kg/s, moisture content 50.37±1.60%, ash content 7.38±1.75% (dry matter), fat content 41.06±6.07% (dry matter), protein content 37.85±3.25% (dry matter), phosphorus content 346.62±25.61 mg/100g (dry matter), calcium content 860.78±87.91 mg/100g (dry matter), white color, regular texture, not flavorfull, salty taste, soft texture, elastic, ordinary preference acceptance.

Reactions of Monoclonal Antibodies against Human Milk Fat Globule Membranes with Embryonal Tissue

1984 ◽  
pp. 1021-1024 ◽  
Author(s):  
U. KOLDOVSKY ◽  
U. WARGALLA ◽  
J. HILKENS ◽  
J. TAYLOR-PAPADIMITRIOU ◽  
PH. HAGEMANN ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Milk ◽  
Milk Fat ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes ◽  
Human Milk Fat

Functional properties of native and cheese whey protein concentrate powders

2007 ◽  
Vol 60 (4) ◽  
pp. 277-285 ◽  
Author(s):  
ANTTI T HEINO ◽  
JANNE O UUSI-RAUVA ◽  
PIRJO R RANTAMÄKI ◽  
OLLI TOSSAVAINEN
Keyword(s):  
Whey Protein ◽  
Functional Properties ◽  
Cheese Whey ◽  
Whey Protein Concentrate ◽  
Protein Concentrate

scholarly journals BIOCHEMICAL AND MORPHOLOGICAL COMPARISON OF PLASMA MEMBRANE AND MILK FAT GLOBULE MEMBRANE FROM BOVINE MAMMARY GLAND

1970 ◽  
Vol 44 (1) ◽  
pp. 80-93 ◽  
Author(s):  
T. W. Keenan ◽  
D. James Morré ◽  
Diane E. Olson ◽  
W. N. Yunghans ◽  
Stuart Patton
Keyword(s):  
Fatty Acid Composition ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Acid Composition ◽  
Milk Fat ◽  
Major Protein ◽  
Thin Sections ◽  
Milk Fat Globule ◽  
Fat Globule ◽  
Milk Fat Globule Membranes

Purified plasma membrane fractions from lactating bovine mammary glands and membranes of milk fat globules from the same source were similar in distribution and fatty acid composition of phospholipids. The sphingomyelin content of the phospholipid fraction of both membranes was higher than in these fractions from other cell components, ß-carotene, a constituent characteristic of milk fat, was present in the lipid fraction of the plasma membrane. Cholesterol esters of plasma membrane were similar in fatty acid composition to those of milk fat globule membranes. Disc electrophoresis of either membrane preparation on polyacrylamide gels revealed a single major protein component characteristic of plasma membrane from other sources. Distinct morphological differences between plasma membrane and milk fat globule membranes were observed in both thin sections and in negatively stained material. Plasma membrane was vesicular in appearance while milk fat globule membranes had a platelike aspect. These observations are consistent with derivation of fat globule membrane from plasma membrane accompanied by structural rearrangement of membrane constituents.

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