Experimental Validation of the Role of Trifluoroethanol as a Nanocrowder

miRNA (microRNA) are short RNA molecules in length from 20 to 24 nucleotides that have been shown to play an important role in regulating gene expression in many different types of human cancer. Meanwhile, miRNA-214 is one of the known miRNAs involved in the formation of nasopharyngeal carcinoma (NPC) through overexpression that promotes proliferation and development of cancer cells. However, in Vietnam, the study of miR-214 related to NPC has not been conducted yet. With the aims to develop the further studies of miR-214 on NPC in Vietnamese patients, in this initial study, we conducted the analysis of miR-214 expression in previous publications, as well as the prediction of miR-214 potential target genes, which involved in many cellular pathways. Here we applied bioinformatics tools to predict miRNAs and their targets, and discuss the role of miR-214 in the context of human cancers. As the results, miR-214 acted as the oncogenic roles in NPC, relevanted to many pathways, such as cell proliferation, apoptosis, metastasis and invasion through the its target genes LTF, Bim, Bax, LINC0086, etc. In conclusion, the use of computional approaches facilitate the further experimental validation of miRNAs in general, particularly miR-214, in Vietnamese NPC patients.

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Elucidation of novel miRNA candidates and their role in unraveling the pathology of Non-Alcoholic Fatty Liver Disease

10.1101/2020.01.31.917831 ◽

2020 ◽

Author(s):

Bincy Mary Biju ◽

Saheb Singh Chhabra ◽

Chiradeep Sarkar

Keyword(s):

Liver Disease ◽

Fatty Liver ◽

Binding Sites ◽

Fatty Liver Disease ◽

Experimental Validation ◽

Alcoholic Fatty Liver ◽

Novel Mirna ◽

Transcriptional Gene Regulation ◽

Alcoholic Fatty Liver Disease

AbstractNon-Alcoholic Fatty Liver Disease (NAFLD) is a chronic liver disease which is observed in people who do not abuse alcohol. Main cause of NAFLD is Non-Alcoholic Steatohepatitis (NASH) where there is accumulation of fats such as triglyceride in liver and the disease progression ranges from simple steatosis to fibrosis and cirrhosis. MicroRNAs are critical players in post-transcriptional gene regulation of diseases with complex etiology. In this study, we have elucidated the role of microRNAs (miRNAs) in the pathophysiology of NAFLD/NASH and unravelled molecular markers for diagnosis of NAFLD. A subset of genes (n=10) responsible for NAFLD/NASH were selected and detailed in silico analysis carried out using multiple tools. miRDB and DIANA-microT were used to find putative miRNA binding sites followed by analysis using miRTarBase which is an experimentally validated database of miRNA-target interactions. The study elucidated a number of statistically significant predictions for both miRDB (scores >80) and DIANA-microT (values >0.90) and also strong experimental validation in miRTarBase. The analysis revealed that certain miRNAs like miR-7 & miR-548 family members are found in both the programmes, miRDB and DIANA-microT, targeting genes involved in liver function. They were also identified in the experimental validation database miRTarBase. These miRNAs probably play an important role in the pathophysiology of this disease. They can also be used as prognostic/diagnostic markers for assessment of NAFLD.

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The Role of Front-End AC/DC Converters in Hybrid AC/DC Smart Homes: Analysis and Experimental Validation

Electronics ◽

10.3390/electronics10212601 ◽

2021 ◽

Vol 10 (21) ◽

pp. 2601

Author(s):

Vitor Monteiro ◽

Luis F. C. Monteiro ◽

Francesco Lo Franco ◽

Riccardo Mandrioli ◽

Mattia Ricco ◽

...

Keyword(s):

Smart Grids ◽

Experimental Validation ◽

Grid Point ◽

Electrical Power ◽

Smart Homes ◽

Electric Mobility ◽

Control Algorithms ◽

New Paradigm ◽

Front End

Electrical power grids are rapidly evolving into smart grids, with smart homes also making an important contribution to this. In fact, the well-known and emerging technologies of renewables, energy storage systems and electric mobility are each time more distributed throughout the power grid and included in smart homes. In such circumstances, since these technologies are natively operating in DC, it is predictable for a revolution in the electrical grid craving a convergence to DC grids. Nevertheless, traditional loads natively operating in AC will continue to be used, highlighting the importance of hybrid AC/DC grids. Considering this new paradigm, this paper has as main innovation points the proposed control algorithms regarding the role of front-end AC/DC converters in hybrid AC/DC smart homes, demonstrating their importance for providing unipolar or bipolar DC grids for interfacing native DC technologies, such as renewables and electric mobility, including concerns regarding the power quality from a smart grid point of view. Furthermore, the paper presents a clear description of the proposed control algorithms, aligned with distinct possibilities of complementary operation of front-end AC/DC converters in the perspective of smart homes framed within smart grids, e.g., enabling the control of smart homes in a coordinated way. The analysis and experimental results confirmed the suitability of the proposed innovative operation modes for hybrid AC/DC smart homes, based on two different AC/DC converters in the experimental validation.

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Experimental Investigation and Modeling of Mudcake Buildup Inside Fractures and the Resulting Fracture Isolation

SPE Journal ◽

10.2118/198895-pa ◽

2019 ◽

Vol 24 (06) ◽

pp. 2929-2945

Author(s):

Hamza A. Jaffal ◽

Kenneth E. Gray ◽

Chadi S. El Mohtar

Keyword(s):

Experimental Investigation ◽

Experimental Validation ◽

Recent Literature ◽

Basic Physics ◽

Fracture Sealing ◽

Wellbore Strengthening

Summary The role of mudcake buildup in fracture sealing and wellbore strengthening has been observed and documented in recent literature. However, there is a gap in the literature when it comes to fully understanding the mechanism of mudcake buildup, whether inside the fracture or at its mouth. This study presents an extension to a previously developed model, along with experimental validation, for mudcake buildup at the wellbore wall to capture the basic physics of mudcake buildup inside fractures and the resulting fracture sealing.

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