Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag; Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles†,‡

Fluorescence behavior of octadecylrhodamine B (ORB) and the association-dependent changes in UV-VIS absorption and fluorescence spectra in aqueous solutions were studied by UV-VIS absorption spectroscopy and steady-state and time-resolved fluorometry. Spectral changes are interpreted in terms of the hydrophobically driven formation of H- and J-dimers and higher aggregates. The knowledge gained serves as a basis for the interpretation of results of the fluorescence correlation spectroscopy study of polymeric micelles labeled with ORB.

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3P-308 Local measurement of cell surfaces with atomic force microscopy and fluorescence correlation spectroscopy(The 46th Annual Meeting of the Biophysical Society of Japan)

Seibutsu Butsuri ◽

10.2142/biophys.48.s175_2 ◽

2008 ◽

Vol 48 (supplement) ◽

pp. S175

Author(s):

Yuuki Kushida ◽

Kenichi Niikura ◽

Ryosuke Kamitani ◽

Yukari Haruna ◽

Yusuke Mizutani ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Annual Meeting ◽

Fluorescence Correlation Spectroscopy ◽

Correlation Spectroscopy ◽

Local Measurement ◽

Cell Surfaces ◽

Force Microscopy ◽

Fluorescence Correlation ◽

Atomic Force ◽

Biophysical Society

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Local Mobility in Lipid Domains of Supported Bilayers Characterized by Atomic Force Microscopy and Fluorescence Correlation Spectroscopy

Biophysical Journal ◽

10.1529/biophysj.105.060327 ◽

2005 ◽

Vol 89 (2) ◽

pp. 1081-1093 ◽

Cited By ~ 84

Author(s):

A.R. Burns ◽

D.J. Frankel ◽

T. Buranda

Keyword(s):

Atomic Force Microscopy ◽

Fluorescence Correlation Spectroscopy ◽

Correlation Spectroscopy ◽

Lipid Domains ◽

Supported Bilayers ◽

Force Microscopy ◽

Fluorescence Correlation ◽

Atomic Force ◽

Local Mobility

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Hybrid Block Copolymer Micelles with Partly Hydrophobically Modified Polyelectrolyte Shells in Polar and Aqueous Media: Experimental Study Using Fluorescence Correlation Spectroscopy, Time-Resolved Fluorescence, Light Scattering, and Atomic Force Microscopy†

The Journal of Physical Chemistry B ◽

10.1021/jp022221s ◽

2003 ◽

Vol 107 (32) ◽

pp. 8232-8240 ◽

Cited By ~ 17

Author(s):

Pavel Matějíček ◽

Jana Humpolíčková ◽

Karel Procházka ◽

Zdeněk Tuzar ◽

Milena Špírková ◽

...

Keyword(s):

Aqueous Media ◽

Correlation Spectroscopy ◽

Time Resolved ◽

Force Microscopy ◽

Fluorescence Correlation ◽

Hydrophobically Modified ◽

Atomic Force ◽

Fluorescence Light ◽

Block Copolymer Micelles ◽

Hydrophobically Modified Polyelectrolyte

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Light Scattering, Atomic Force Microscopy and Fluorescence Correlation Spectroscopy Studies of Polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide) Micelles

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc20032120 ◽

2003 ◽

Vol 68 (11) ◽

pp. 2120-2138 ◽

Cited By ~ 23

Author(s):

Miroslav Štěpánek ◽

Jana Humpolíčková ◽

Karel Procházka ◽

Martin Hof ◽

Zdeněk Tuzar ◽

...

Keyword(s):

Atomic Force Microscopy ◽

Light Scattering ◽

Fluorescence Correlation Spectroscopy ◽

Polymeric Nanoparticles ◽

Correlation Spectroscopy ◽

Force Microscopy ◽

Fluorescence Correlation ◽

Atomic Force ◽

Poly Ethylene Oxide ◽

Poly Ethylene

Polymeric nanoparticles formed by triblock copolymer polystyrene-block-poly(2-vinylpyridine)-block-poly(ethylene oxide), PS-PVP-PEO, in aqueous media were studied by a combination of fluorescence correlation spectroscopy with other fluorescence techniques, light scattering and atomic force microscopy. The studied polymeric nanoparticles exist in the form of (i) core/shell micelles in acid solution at pH lower than 4.8 and (ii) three-layer onion micelles at higher pH. Since water is a very strong precipitant for PS, both types of micelles have kinetically frozen spherical PS cores. The cores of micelles in acid media are surrounded by soluble shells formed by partly protonated PVP and PEO, while the cores of micelles in alkaline media are surrounded by compact insoluble layers of deprotonated PVP and soluble PEO shells. The micellization behavior of PS-PVP-PEO micelles is accompanied by secondary aggregation of micelles, which is provoked by stirring, shaking and also by filtration of micellar solutions. Therefore fluorescence correlation spectroscopy (FCS), which, in contrast to light scattering techniques, does not require filtration, was used as the main experimental technique for the characterization of non-aggregated micelles. The binding of a fluorescence probe, octadecylrhodamine B (ORB), to polymeric micelles, was studied before the FCS study of micelles.

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