Specific Interactions of Mycosubtilin with Cholesterol-Containing Artificial Membranes

We have employed cryoelectron microscopy to visualize events that occur during the freeze-drying of artificial membranes by employing real time video capture techniques. Artificial membranes or liposomes which are spherical structures within internal aqueous space are stabilized by water which provides the driving force for spontaneous self-assembly of these structures. Previous assays of damage to these structures which are induced by freeze drying reveal that the two principal deleterious events that occur are 1) fusion of liposomes and 2) leakage of contents trapped within the liposome [1]. In the past the only way to access these events was to examine the liposomes following the dehydration event. This technique allows the event to be monitored in real time as the liposomes destabilize and as water is sublimed at cryo temperatures in the vacuum of the microscope. The method by which liposomes are compromised by freeze-drying are largely unknown. This technique has shown that cryo-protectants such as glycerol and carbohydrates are able to maintain liposomal structure throughout the drying process.

Download Full-text

Fragment MO Calculations on Specific Interactions between AhR and TCDD for Beluga and Tern

Journal of Computer Aided Chemistry ◽

10.2751/jcac.10.63 ◽

2009 ◽

Vol 10 ◽

pp. 63-75

Author(s):

Satoshi Miyagi ◽

Eri Yoshikawa ◽

Kenichi Dedachi ◽

Satoshi Itoh ◽

Mitsuko Ishihara-Sugano ◽

...

Keyword(s):

Specific Interactions ◽

Mo Calculations

Download Full-text

Faculty Opinions recommendation of Non-sequence-specific interactions can account for the compaction of proteins unfolded under "native" conditions.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1432956.910054 ◽

2010 ◽

Author(s):

Kunihiro Kuwajima

Keyword(s):

Specific Interactions

Download Full-text

Faculty Opinions recommendation of Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.725495854.793533938 ◽

2017 ◽

Author(s):

Pascale Cossart

Keyword(s):

Listeria Monocytogenes ◽

Host Cells ◽

Specific Interactions

Download Full-text

Yield and inter-specific interactions in maize/chickpea intercrop under different application rates of P in irrigated sierozem along the Yellow River in Northwest China

CHINESE JOURNAL OF ECO-AGRICULTURE ◽

10.3724/sp.j.1011.2010.00917 ◽

2010 ◽

Vol 18 (5) ◽

pp. 917-922

Author(s):

Yu-Feng LAN ◽

Hai-Yong XIA ◽

Hong-Liang LIU ◽

Si-Cun YANG ◽

Jian-Guo SONG ◽

...

Keyword(s):

Yellow River ◽

Northwest China ◽

The Yellow River ◽

Specific Interactions ◽

Application Rates

Download Full-text

Specific interactions in solutions of polymers. V. An interpretation of the enthalpies of mixing of chloroform with butyl methacrylate oligomers in terms of Barker's theory

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19733123 ◽

1973 ◽

Vol 38 (10) ◽

pp. 3123-3133 ◽

Cited By ~ 1

Author(s):

Z. Máša ◽

A. Živný ◽

J. Biroš

Keyword(s):

Butyl Methacrylate ◽

Specific Interactions ◽

Enthalpies Of Mixing

Download Full-text

Influence of medium on alkaline hydrolysis of succinic acid monomethyl and monopropyl esters

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19802873 ◽

1980 ◽

Vol 45 (11) ◽

pp. 2873-2882

Author(s):

Vladislav Holba ◽

Ján Benko

Keyword(s):

Succinic Acid ◽

Kinetic Parameters ◽

Alkaline Hydrolysis ◽

Specific Interactions ◽

Nonaqueous Media ◽

The Media ◽

Kinetics Of ◽

Hydrolysis Of

The kinetics of alkaline hydrolysis of succinic acid monomethyl and monopropyl esters were studied in mixed aqueous-nonaqueous media at various temperatures and ionic strengths. The results of measurements are discussed in terms of electrostatic and specific interactions between the reactants and other components of the reaction mixture. The kinetic parameters in the media under study are related to the influence of the cosolvent on the solvation sphere of the reactants.

Download Full-text

Omp35, a New Enterobacter aerogenes Porin Involved in Selective Susceptibility to Cephalosporins

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.48.6.2153-2158.2004 ◽

2004 ◽

Vol 48 (6) ◽

pp. 2153-2158 ◽

Cited By ~ 25

Author(s):

Charléric Bornet ◽

Nathalie Saint ◽

Lilia Fetnaci ◽

Myrielle Dupont ◽

Anne Davin-Régli ◽

...

Keyword(s):

Membrane Permeability ◽

Outer Membrane ◽

Antibiotic Susceptibility ◽

Enterobacter Aerogenes ◽

Artificial Membranes ◽

Specific Location ◽

Outer Membrane Permeability ◽

Constriction Region ◽

High Conductance

ABSTRACT In Enterobacter aerogenes, β-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. In ATCC 15038 strain, we observed a different pattern of porin production associated with a variable antibiotic susceptibility. We purified Omp35, which is expressed under conditions of low osmolality and analyzed its pore-forming properties in artificial membranes. This porin was found to be an OmpF-like protein with high conductance values. It showed a noticeably higher conductance compared to Omp36 and a specific location of WNYT residues in the L3 loop. The importance of the constriction region in the porin function suggests that this organization is involved in the level of susceptibility to negative large cephalosporins such as ceftriaxone by bacteria producing the Omp35 porin subfamily.

Download Full-text