Specific Interactions of Mycosubtilin with Cholesterol-Containing Artificial Membranes

Langmuir ◽  
2011 ◽  
Vol 27 (17) ◽  
pp. 10785-10792 ◽  
Author(s):  
Mehmet Nail Nasir ◽  
Françoise Besson
Keyword(s):  
Specific Interactions ◽  
Artificial Membranes

Video real-time imaging of artificial membranes during freeze-drying by cryo-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 16-17
Author(s):  
Alan S. Rudolph ◽  
Ronald R. Price
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Freeze Drying ◽  
Video Capture ◽  
Drying Process ◽  
Artificial Membranes ◽  
The Past ◽  
Cryo Electron Microscopy ◽  
Spherical Structures ◽  
Capture Techniques

We have employed cryoelectron microscopy to visualize events that occur during the freeze-drying of artificial membranes by employing real time video capture techniques. Artificial membranes or liposomes which are spherical structures within internal aqueous space are stabilized by water which provides the driving force for spontaneous self-assembly of these structures. Previous assays of damage to these structures which are induced by freeze drying reveal that the two principal deleterious events that occur are 1) fusion of liposomes and 2) leakage of contents trapped within the liposome [1]. In the past the only way to access these events was to examine the liposomes following the dehydration event. This technique allows the event to be monitored in real time as the liposomes destabilize and as water is sublimed at cryo temperatures in the vacuum of the microscope. The method by which liposomes are compromised by freeze-drying are largely unknown. This technique has shown that cryo-protectants such as glycerol and carbohydrates are able to maintain liposomal structure throughout the drying process.

Fragment MO Calculations on Specific Interactions between AhR and TCDD for Beluga and Tern

2009 ◽  
Vol 10 ◽  
pp. 63-75
Author(s):  
Satoshi Miyagi ◽  
Eri Yoshikawa ◽  
Kenichi Dedachi ◽  
Satoshi Itoh ◽  
Mitsuko Ishihara-Sugano ◽  
...  
Keyword(s):  
Specific Interactions ◽  
Mo Calculations

Yield and inter-specific interactions in maize/chickpea intercrop under different application rates of P in irrigated sierozem along the Yellow River in Northwest China

2010 ◽  
Vol 18 (5) ◽  
pp. 917-922
Author(s):  
Yu-Feng LAN ◽  
Hai-Yong XIA ◽  
Hong-Liang LIU ◽  
Si-Cun YANG ◽  
Jian-Guo SONG ◽  
...  
Keyword(s):  
Yellow River ◽  
Northwest China ◽  
The Yellow River ◽  
Specific Interactions ◽  
Application Rates

Influence of medium on alkaline hydrolysis of succinic acid monomethyl and monopropyl esters

1980 ◽  
Vol 45 (11) ◽  
pp. 2873-2882
Author(s):  
Vladislav Holba ◽  
Ján Benko
Keyword(s):  
Succinic Acid ◽  
Kinetic Parameters ◽  
Alkaline Hydrolysis ◽  
Specific Interactions ◽  
Nonaqueous Media ◽  
The Media ◽  
Kinetics Of ◽  
Hydrolysis Of

The kinetics of alkaline hydrolysis of succinic acid monomethyl and monopropyl esters were studied in mixed aqueous-nonaqueous media at various temperatures and ionic strengths. The results of measurements are discussed in terms of electrostatic and specific interactions between the reactants and other components of the reaction mixture. The kinetic parameters in the media under study are related to the influence of the cosolvent on the solvation sphere of the reactants.

scholarly journals Omp35, a New Enterobacter aerogenes Porin Involved in Selective Susceptibility to Cephalosporins

2004 ◽  
Vol 48 (6) ◽  
pp. 2153-2158 ◽  
Author(s):  
Charléric Bornet ◽  
Nathalie Saint ◽  
Lilia Fetnaci ◽  
Myrielle Dupont ◽  
Anne Davin-Régli ◽  
...  
Keyword(s):  
Membrane Permeability ◽  
Outer Membrane ◽  
Antibiotic Susceptibility ◽  
Enterobacter Aerogenes ◽  
Artificial Membranes ◽  
Specific Location ◽  
Outer Membrane Permeability ◽  
Constriction Region ◽  
High Conductance

ABSTRACT In Enterobacter aerogenes, β-lactam resistance often involves a decrease in outer membrane permeability induced by modifications of porin synthesis. In ATCC 15038 strain, we observed a different pattern of porin production associated with a variable antibiotic susceptibility. We purified Omp35, which is expressed under conditions of low osmolality and analyzed its pore-forming properties in artificial membranes. This porin was found to be an OmpF-like protein with high conductance values. It showed a noticeably higher conductance compared to Omp36 and a specific location of WNYT residues in the L3 loop. The importance of the constriction region in the porin function suggests that this organization is involved in the level of susceptibility to negative large cephalosporins such as ceftriaxone by bacteria producing the Omp35 porin subfamily.

Specific Interactions and Environment Flexibility Tune Protein Stability under Extreme Crowding

2021 ◽  
Author(s):  
Marina Katava ◽  
Guillaume Stirnemann ◽  
Maria Pachetti ◽  
Simone Capaccioli ◽  
Alessandro Paciaroni ◽  
...  
Keyword(s):  
Protein Stability ◽  
Specific Interactions

scholarly journals Tissue-specific interactions between nuclear proteins and the aminopeptidase N promoter.

1991 ◽  
Vol 266 (27) ◽  
pp. 18089-18096
Author(s):  
J. Olsen ◽  
L. Laustsen ◽  
U. Kärnström ◽  
H. Sjöström ◽  
O. Norén
Keyword(s):  
Aminopeptidase N ◽  
Nuclear Proteins ◽  
Specific Interactions ◽  
Tissue Specific
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