Spherical Phospholipid Polymer Hydrogels for Cell Encapsulation Prepared with a Flow-Focusing Microfluidic Channel Device

Langmuir ◽  
2011 ◽  
Vol 28 (4) ◽  
pp. 2145-2150 ◽  
Author(s):  
Tatsuo Aikawa ◽  
Tomohiro Konno ◽  
Madoka Takai ◽  
Kazuhiko Ishihara
Keyword(s):  
Microfluidic Channel ◽  
Cell Encapsulation ◽  
Flow Focusing ◽  
Polymer Hydrogels ◽  
Phospholipid Polymer

scholarly journals Shrinking microbubbles with microfluidics: mathematical modelling to control microbubble sizes

2021 ◽  
Author(s):  
A. Salari ◽  
V. Gnyawali ◽  
I. M. Griffiths ◽  
R. Karshafian ◽  
Michael C. Kolios ◽  
...  
Keyword(s):  
Life Sciences ◽  
Microfluidic Channel ◽  
Flow Focusing ◽  
Bubble Generation ◽  
Microfluidic Platform ◽  
Interfacial Tensions ◽  
Precise Control ◽  
Ultrasound Diagnostics ◽  
Bubble Sizes ◽  
Good Agreement

Microbubbles have applications in industry and life-sciences. In medicine, small encapsulated bubbles (< 10 μm) are desirable because of their utility in drug/oxygen delivery, sonoporation, and ultrasound diagnostics. While there are various techniques for generating microbubbles, microfluidic methods are distinguished due to their precise control and ease-offabrication. Nevertheless, sub-10 μm diameter bubble generation using microfluidics remains challenging, and typically requires expensive equipment and cumbersome setups. Recently, our group reported a microfluidic platform that shrinks microbubbles to sub-10 μm diameters. The microfluidic platform utilizes a simple microbubble-generating flow-focusing geometry, integrated with a vacuum shrinkage system, to achieve microbubble sizes that are desirable in medicine, and pave the way to eventual clinical uptake of microfluidically generated microbubbles. A theoretical framework is now needed to relate the size of the microbubbles produced and the system’s input parameters. In this manuscript, we characterize microbubbles made with various lipid concentrations flowing in solutions that have different interfacial tensions, and monitor the changes in bubble size along the microfluidic channel under various vacuum pressures. We use the physics governing the shrinkage mechanism to develop a mathematical model that predicts the resulting bubble sizes and elucidates the dominant parameters controlling bubble sizes. The model shows a good agreement with the experimental data, predicting the resulting microbubble sizes under different experimental input conditions. We anticipate that the model will find utility in enabling users of the microfluidic platform to engineer bubbles of specific sizes.

Injectable Acylhydrazone‐linked RAFT Polymer Hydrogels for Sustained Protein Release and Cell Encapsulation

2021 ◽  
pp. 2101284
Author(s):  
Fang‐Yi Lin ◽  
Nathan H. Dimmitt ◽  
Mariana Moraes de Lima Perini ◽  
Jiliang Li ◽  
Chien‐Chi Lin
Keyword(s):  
Cell Encapsulation ◽  
Protein Release ◽  
Polymer Hydrogels ◽  
Sustained Protein Release

Prevention of Tissue Adhesion by a Spontaneously Formed Phospholipid Polymer Hydrogel

2007 ◽  
Vol 342-343 ◽  
pp. 777-780 ◽  
Author(s):  
Mizuna Kimura ◽  
Tomohiro Konno ◽  
Madoka Takai ◽  
Noriyuki Ishiyama ◽  
Toru Moro ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Aqueous Solutions ◽  
Viscoelastic Properties ◽  
Gelation Time ◽  
Butyl Methacrylate ◽  
Tissue Adhesion ◽  
Polymer Hydrogels ◽  
Physiological Conditions ◽  
Phospholipid Polymer

We investigated phospholipid polymer hydrogels containing Fe3+ ions (PMA/PMB/Fe hydrogel) for their use as antiadhesive materials in the healing tissues. These hydrogels were prepared from the aqueous solutions of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)-comethacrylic acid) (PMA) and poly(MPC-co-n-butyl methacrylate) (PMB). The PMA/PMB hydrogel is formed by the intermolecular interactions between PMA and PMB, and it reversibly dissociates under physiological conditions. The addition of Fe3+ ions could control the gelation time and the dissociation time. Mechanical properties such as the gelation time and viscoelastic properties can be controlled by the FeCl3 concentration. With regard to biocompatibility, no evidence of inflammation was observed in vivo. Therefore, the PMA/PMB/Fe hydrogel has a potential to be used as an antiadhesive material.

The use of the mechanical microenvironment of phospholipid polymer hydrogels to control cell behavior

Biomaterials ◽  
2013 ◽  
Vol 34 (24) ◽  
pp. 5891-5896 ◽  
Author(s):  
Haruka Oda ◽  
Tomohiro Konno ◽  
Kazuhiko Ishihara
Keyword(s):  
Control Cell ◽  
Cell Behavior ◽  
Polymer Hydrogels ◽  
Phospholipid Polymer ◽  
Mechanical Microenvironment

scholarly journals Cytocompatible and reversible phospholipid polymer hydrogels for encapsulation to provide unified quality cells

2014 ◽  
Vol 39 (3) ◽  
pp. 279-282
Author(s):  
Haruka Oda ◽  
Tomohiro Konno ◽  
Kazuhiko Ishihara
Keyword(s):  
Polymer Hydrogels ◽  
Phospholipid Polymer

scholarly journals Novel tissue-binding phospholipid polymer hydrogels for mucosal tissue regeneration

2016 ◽  
Vol 4 ◽  
Author(s):  
Mcminn Mariah ◽  
Oda Haruka ◽  
Ishihara Kazuhiko ◽  
Nagatomi Jiro
Keyword(s):  
Tissue Regeneration ◽  
Tissue Binding ◽  
Mucosal Tissue ◽  
Polymer Hydrogels ◽  
Phospholipid Polymer

scholarly journals Three step flow focusing enables image-based discrimination and sorting of late stage 1 Haematococcus pluvialis cells

PLoS ONE ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. e0249192
Author(s):  
Daniel Kraus ◽  
Andreas Kleiber ◽  
Enrico Ehrhardt ◽  
Matthias Leifheit ◽  
Peter Horbert ◽  
...  
Keyword(s):  
Late Stage ◽  
Haematococcus Pluvialis ◽  
Lateral Displacement ◽  
Microfluidic Channel ◽  
Image Features ◽  
Target Cells ◽  
Flow Focusing ◽  
Step Flow ◽  
Stage 1 ◽  
Mixed Stage

Label-free and gentle separation of cell stages with desired target properties from mixed stage populations are a major research task in modern biotechnological cultivation process and optimization of micro algae. The reported microfluidic sorter system (MSS) allows the subsequent investigation of separated subpopulations. The implementation of a viability preserving MSS is shown for separation of late stage 1 Haematococcus pluvialis (HP) cells form a mixed stage population. The MSS combines a three-step flow focusing unit for aligning the cells in single file transportation mode at the center of the microfluidic channel with a pure hydrodynamic sorter structure for cell sorting. Lateral displacement of the cells into one of the two outlet channels is generated by piezo-actuated pump chambers. In-line decision making for sorting is based on a user-definable set of image features and properties. The reported MSS significantly increased the purity of target cells in the sorted population (94%) in comparison to the initial mixed stage population (19%).

The biological performance of cell-containing phospholipid polymer hydrogels in bulk and microscale form

Biomaterials ◽  
2010 ◽  
Vol 31 (34) ◽  
pp. 8839-8846 ◽  
Author(s):  
Yan Xu ◽  
Kihoon Jang ◽  
Tomohiro Konno ◽  
Kazuhiko Ishihara ◽  
Kazuma Mawatari ◽  
...  
Keyword(s):  
Polymer Hydrogels ◽  
Phospholipid Polymer ◽  
Biological Performance

Biocompatibility and drug release behavior of spontaneously formed phospholipid polymer hydrogels

2007 ◽  
Vol 80A (1) ◽  
pp. 45-54 ◽  
Author(s):  
Mizuna Kimura ◽  
Madoka Takai ◽  
Kazuhiko Ishihara
Keyword(s):  
Drug Release ◽  
Release Behavior ◽  
Polymer Hydrogels ◽  
Drug Release Behavior ◽  
Phospholipid Polymer
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