Detection and Quantification of Specific DNA Adducts by Liquid Chromatography−Tandem Mass Spectrometry in the Livers of Rats Given Estragole at the Carcinogenic Dose

2011 ◽  
Vol 24 (4) ◽  
pp. 532-541 ◽  
Author(s):  
Yuji Ishii ◽  
Yuta Suzuki ◽  
Daisuke Hibi ◽  
Meilan Jin ◽  
Kiyoshi Fukuhara ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Dna Adducts ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Detection And Quantification

Quantification of benzo[a]pyrene diol epoxide DNA-adducts by stable isotope dilution liquid chromatography/tandem mass spectrometry

2006 ◽  
Vol 20 (8) ◽  
pp. 1369-1380 ◽  
Author(s):  
Qian Ruan ◽  
Hye-Young H. Kim ◽  
Hao Jiang ◽  
Trevor M. Penning ◽  
Ronald G. Harvey ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Stable Isotope ◽  
Tandem Mass Spectrometry ◽  
Dna Adducts ◽  
Isotope Dilution ◽  
Tandem Mass ◽  
Stable Isotope Dilution ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

scholarly journals Detection and quantification of metastable photoproducts of trenbolone and altrenogest using liquid chromatography–tandem mass spectrometry

2019 ◽  
Vol 1603 ◽  
pp. 150-159 ◽  
Author(s):  
Philip T. Kenyon ◽  
Haoqi Zhao ◽  
Xingjian Yang ◽  
Christopher Wu ◽  
David M. Cwiertny ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Detection And Quantification

scholarly journals A liquid chromatography⿿tandem mass spectrometry assay for the detection and quantification of trehalose in biological samples

2016 ◽  
Vol 1033-1034 ◽  
pp. 9-16 ◽  
Author(s):  
Philip M. Kretschmer ◽  
Austin M. Bannister ◽  
Molly K. O⿿Brien ◽  
Laura A. MacManus-Spencer ◽  
Margot G. Paulick
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Biological Samples ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Detection And Quantification ◽  
Mass Spectrometry Assay

scholarly journals Evaluation of inhaled low-dose formaldehyde-induced DNA adducts and DNA–protein cross-links by liquid chromatography–tandem mass spectrometry

2019 ◽  
Vol 93 (3) ◽  
pp. 763-773 ◽  
Author(s):  
Jiapeng Leng ◽  
Chih-Wei Liu ◽  
Hadley J. Hartwell ◽  
Rui Yu ◽  
Yongquan Lai ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Dna Adducts ◽  
Low Dose ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Cross Links

scholarly journals Increasing Liquid Chromatography–Tandem Mass Spectrometry Throughput by Mass Tagging: A Sample-Multiplexed High-Throughput Assay for 25-Hydroxyvitamin D2 and D3

2011 ◽  
Vol 57 (3) ◽  
pp. 431-440 ◽  
Author(s):  
Brian C Netzel ◽  
Kendall W Cradic ◽  
Eric T Bro ◽  
Adam B Girtman ◽  
Richard C Cyr ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Limits Of Detection ◽  
Sample Throughput ◽  
Chromatography Tandem Mass Spectrometry ◽  
Deming Regression ◽  
Liquid Chromatography Tandem Mass ◽  
Detection And Quantification

BACKGROUND The limits of chromatographic speed and mechanical frontend capabilities have been reached for many high-volume liquid chromatography–tandem mass spectrometry (LC-MS/MS) tests, curtailing the maximal achievable sample throughput. To overcome these boundaries, we developed and validated a derivatization-based sample-multiplex LC-MS/MS assay for detection of 25-hydroxyvitamins D2 and D3 [25(OH)D2 and 25(OH)D3], which increased sample throughput 5-fold. METHODS After separate derivatization with 1 of 5 different triazoline-diones (TADs), 5 calibrators, controls, or patient specimens were combined and injected together into an LC-MS/MS. On the basis of mass differences between TADs, the MS/MS quantified analyte and stable isotope internal standards for 25(OH)D2 and 25(OH)D3 for each respective multiplexed sample within the injection. Limits of detection and quantification, spiked recovery, linearity, imprecision, and patient results were determined and compared against our standard LC-MS/MS assay. RESULTS TAD multiplexing increased throughput on an LC-quadruplexed LC-MS/MS system from 60 samples/h to 300 samples/h. Limits of detection and quantification were 4.9 nmol/L [2 μg/L, 25(OH)D2], 2.2 nmol/L [0.9 μg/L, 25(OH)D3], and 10 nmol/L [4 μg/L, 25(OH)D2], 5 nmol/L [2 μg/L, 25(OH)D3], respectively. The assay was linear to 250 nmol/L (100 μg/L). Interassay CVs across the reportable range were 3.7%–15.2%. Spiked recoveries were 94%–119%. The method comparison with the standard LC-MS/MS method showed slopes of 0.96 and 0.97 (Deming regression) for 25(OH)D2 (n = 1733) and 25(OH)D3 (n = 7614) (R2=0.96 and 0.97), respectively. CONCLUSIONS Multiplexing samples by differential mass tagging in LC-MS/MS measurement of 25(OH)D2 and 25(OH)D3 allows for reliable quantification, with throughput increased over standard methods by the multiplexing factor.

scholarly journals DNA adducts of 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine and 4-aminobiphenyl are infrequently detected in human mammary tissue by liquid chromatography/tandem mass spectrometry

2011 ◽  
Vol 33 (1) ◽  
pp. 124-130 ◽  
Author(s):  
Dan Gu ◽  
Robert J. Turesky ◽  
Yeqing Tao ◽  
Sophie A. Langouët ◽  
Gwendoline C. Nauwelaërs ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Dna Adducts ◽  
Mammary Tissue ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass
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