Specific Degradation of D1 Protein during Exposure of Thylakoid Membranes to High Temperature in the Dark

Photosynthetic capacity and photosystem II (PSII) activity decreased with increasing temperature, whereas antioxidant enzyme activity showed the opposite trend. High temperature stress induced a significant increase in Φf,D, and D1 protein turnover rate. Photosynthetic capacity, PSII activity, and antioxidant enzyme levels in plants treated at 35 and 40°C were restored to control levels upon stress relief, whereas those in plants grown at 45℃ were only partially restored. Therefore, the temperature limit for heat tolerance in Parthenocissus quinquefolia is between 40 and 45℃. Further, it was observed that antioxidant enzymes were crucial for high-temperature stress resistance in P. quinquefolia, with DEGP1 protein playing a major role in the rapid turnover of D1 protein for PSII repair. Bangladesh J. Bot. 50(2): 433-436, 2021 (June)

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Degradation of unassembled and damaged thylakoid proteins

Biochemical Society Transactions ◽

10.1042/bst0290427 ◽

2001 ◽

Vol 29 (4) ◽

pp. 427-430 ◽

Cited By ~ 13

Author(s):

Z. Adam ◽

O. Ostersetzer

Keyword(s):

Thylakoid Membrane ◽

D1 Protein ◽

Thylakoid Membranes ◽

Reaction Centre ◽

Ps Ii ◽

Plant Genomes ◽

Thylakoid Membrane Proteins ◽

Luminal Side ◽

Cytochrome Complex ◽

Aaa Protein

To study protein degradation in thylakoid membranes we identified, characterized and cloned thylakoid proteases, and then linked them to known proteolytic processes. Several families of chloroplast proteases were identified and characterized to different extents. FtsH, an ATP-dependent metalloprotease that belongs to the AAA-protein family, was found to be integral to the thylakoid membrane, facing the stroma. It is involved in both the degradation of unassembled subunits of membrane complexes, such as the Rieske Fe-S protein of the cytochrome complex, and the degradation of oxidatively damaged proteins such as the D1 protein of the photosystem II (PS II) reaction centre. Plant genomes contain multiple isomers of this protease but the functional significance of this multiplication is not clear yet. A second protease, the serine ATP-independent DegP, was found to be strongly associated with the luminal side of the thylakoid membrane. Although a specific role has not yet assigned for it, its location suggests that it can degrade luminal soluble proteins as well as luminally exposed regions of thylakoid membrane proteins.

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High temperature effects on D1 protein turnover in three wheat varieties with different heat susceptibility

Plant Growth Regulation ◽

10.1007/s10725-016-0179-6 ◽

2016 ◽

Vol 81 (1) ◽

pp. 1-9 ◽

Cited By ~ 19

Author(s):

Hua Li ◽

Hailiang Xu ◽

Pengju Zhang ◽

Mingquan Gao ◽

Dan Wang ◽

...

Keyword(s):

High Temperature ◽

Protein Turnover ◽

Temperature Effects ◽

D1 Protein ◽

Wheat Varieties

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PROTECTIVE ROLES OF D1 PROTEIN TURNOVER AND THE XANTHOPHYLL CYCLE IN TOMATO (SOLANUM LYCOPERSICUM) UNDER SUB-HIGH TEMPERATURE AND HIGH LIGHT STRESS

Frontiers of Agricultural Science and Engineering ◽

10.15302/j-fase-2021383 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0

Keyword(s):

High Temperature ◽

Solanum Lycopersicum ◽

Xanthophyll Cycle ◽

Protein Turnover ◽

Light Stress ◽

D1 Protein ◽

High Light ◽

High Light Stress

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Degradation of proteins from thylakoid membranes in senescing wheat leaves at high temperature

Plant Cell & Environment ◽

10.1111/j.1365-3040.1993.tb00888.x ◽

1993 ◽

Vol 16 (4) ◽

pp. 421-428 ◽

Cited By ~ 9

Author(s):

D. L. FERGUSON* ◽

K. AL-KHATIB ◽

J. A. GUIKEMA ◽

G. M. PAULSEN

Keyword(s):

High Temperature ◽

Thylakoid Membranes ◽

Wheat Leaves

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Hydroxyl radicals are not the protagonists of UV-B-induced damage in isolated thylakoid membranes

Functional Plant Biology ◽

10.1071/fp07151 ◽

2007 ◽

Vol 34 (12) ◽

pp. 1112 ◽

Cited By ~ 10

Author(s):

Iva Šnyrychová ◽

Péter B. Kós ◽

Éva Hideg

Keyword(s):

Hydrogen Peroxide ◽

Electron Transport ◽

Hydroxyl Radicals ◽

Early Stage ◽

D1 Protein ◽

Photosynthetic Electron Transport ◽

Thylakoid Membranes ◽

Oxygen Species ◽

Free Hydrogen ◽

By Products

The production of reactive oxygen species (ROS) was studied in isolated thylakoid membranes exposed to 312 nm UV-B irradiation. Hydroxyl radicals (•OH) and hydrogen peroxide were measured directly, using a newly developed method based on hydroxylation of terephthalic acid and the homovanillic acid/peroxidase assay, respectively. At the early stage of UV-B stress (doses lower than 2.0 J cm–2), •OH were derived from superoxide radicals via hydrogen peroxide. Production of these ROS was dependent on photosynthetic electron transport and was not exclusive to UV-B. Both ROS were found in samples exposed to the same doses of PAR, suggesting that the observed ROS are by-products of the UV-B-driven electron transport rather than specific initiators of the UV-B-induced damage. After longer exposure of thylakoids to UV-B, leading to the inactivation of PSII centres, a small amount of •OH was still observed in thylakoids, even though no free hydrogen peroxide was detected. At this late stage of UV-B stress, •OH may also be formed by the direct cleavage of organic peroxides by UV-B. Immunodetection showed that the presence of the observed ROS alone was not sufficient to achieve the degradation of the D1 protein of PSII centres.

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