Ex Vitro Phenotype Stability is Affected by In Vitro Cultivation

2001 ◽  
Vol 44 (3) ◽  
pp. 321-324 ◽  
Author(s):  
D. Haisel ◽  
P. Hofman ◽  
M. Vagner ◽  
H. Lipavska ◽  
I. Ticha ◽  
...  
Keyword(s):  
In Vitro Cultivation ◽  
Ex Vitro

scholarly journals Diazotrophic bacteria in the growth of micropropagated ornamental pineapple

2019 ◽  
Vol 13 (2) ◽  
pp. 269-278
Author(s):  
Adriano Bortolotti Silva ◽  
Ligiane Aparecida Florentino ◽  
Dalvana De Sousa Pereira ◽  
Paulo Roberto Correa Landgraf ◽  
Ana Carolina Rodrigues Alves ◽  
...  
Keyword(s):  
Shoot Growth ◽  
Ex Vivo ◽  
Control Treatment ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Diazotrophic Bacteria ◽  
Bacterial Strains ◽  
Indole 3 Acetic Acid

Ornamental pineapple is a hardy plant with significant landscaping value. Tissue culture of plants is viable for producing plants with a high phytosanitary quality. However, one of the difficulties with this cultivar is the acclimatization process, which is slow and can cause losses. The objective of the present study was to verify the potential of inoculation with diazotrophic bacteria for in vitro and ex vivo growth of ornamental pineapple. A group of diazotrophic bacterial strains selected at the Universidade José do Rosário Vellano (UNIFENAS) was prioritized in this study, and the treatments included bacterial strains UNIFENAS (100-13, 100-60, 100-68, 100-153, 100-167 and 100-198). These strains were evaluated in terms of their capacity to produce indole 3-acetic acid. Subsequently, plants were cultivated in a medium composed of MS medium salts (1/4), adding 1 mL of the bacterial strain. In the control treatment, the plants were maintained in 2 mL of MS medium. 7 days after inoculation, the plants were transplanted into the MS, where they were maintained for 30 days. After in vitro cultivation, the plants were transferred to pots containing commercial Plantmax® substrate and maintained under these conditions for 60 days. The diazotrophic bacteria were able to synthesize auxins, and their inoculation promoted greater growth in vitro and ex vitro in the plants. In the acclimatization phase, the plants inoculated with UNIFENAS strains (100-60, 100-68 and 100-153) promoted a higher shoot growth, chlorophyll content and nitrate reductase enzyme activity.

scholarly journals In Vitro Multiplication and NMR Fingerprinting of Rare Veronica caucasica M. Bieb

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5888
Author(s):  
Desislava I. Mantovska ◽  
Miroslava K. Zhiponova ◽  
Milen I. Georgiev ◽  
Tsvetinka Grozdanova ◽  
Dessislava Gerginova ◽  
...  
Keyword(s):  
Ex Situ ◽  
Nodal Segments ◽  
Cultivated Plants ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Ex Vitro ◽  
Biotechnological Approach ◽  
Iridoid Glucosides ◽  
Development In Vitro

Micropropagation of rare Veronica caucasica M. Bieb. was achieved by successful in vitro cultivation of mono-nodal segments on MS medium supplemented with 1.0 mg L–1 6-benzylaminopurine (BA) and then transferring the regenerated plants on hormone free basal MS medium for root development. In vitro multiplicated plants were successively acclimated in a growth chamber and a greenhouse with 92% survival. The number of plastid pigments and the total phenolics content in in vitro cultivated and ex vitro adapted plants were unchanged, and no accumulation of reactive oxygen species (ROS) was detected by staining with 3-3′-diaminobenzidine (DAB) and 2′,7′-dichlorofluorescein diacetate (DCF-DA). Nuclear Magnetic Resonance (NMR) fingerprinting allowed for the identification of the major alterations in metabolome of V. caucasica plants during the process of ex situ conservation. Iridoid glucosides such as verproside, aucubin and catalpol were characteristic for in vitro cultivated plants, while in ex vitro acclimated plants phenolic acid–protocatechuic acid and caffeic acid appeared dominant. The successful initiation of in vitro and ex vitro cultures is an alternative biotechnological approach for the preservation of V. caucasica and would allow for further studies of the biosynthetic potential of the species and the selection of lines with a high content of pharmaceutically valuable molecules and nutraceuticals.

scholarly journals Nitrogen assimilation in the bromeliad Ananas comosus var. ananassoides (Baker) Coppens & F.Leal grown in vitro with different sources of inorganic nitrogen

Hoehnea ◽  
2020 ◽  
Vol 47 ◽  
Author(s):  
Priscila Primo Andrade Silva ◽  
Ivomar Aparecido Medina ◽  
Jorge Luiz Marx Young ◽  
Vívian Tamaki
Keyword(s):  
Nitrogen Assimilation ◽  
Inorganic Nitrogen ◽  
Ananas Comosus ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Ex Vitro ◽  
N Assimilation ◽  
Modified Ms Medium ◽  
Different Sources

ABSTRACT Ananas comosus var. ananassoides (Baker) Coppens & F.Leal is a native ornamental bromeliad of the endangered biome Cerrado. Therefore, approaches aimed at the preservation of this species, such as in vitro cultivation and micropropagation are needed. Nitrogen (N) is absorbed by plants, mainly as NO3- and/or NH4+, and assimilated into amino acids. The aim of this work was to evaluate the N assimilation in this bromeliad. Plants were grown in vitro for seven months in modified MS medium with 15, 30, 60, and 90 mM of N as NO3-, NH4+ or NH4NO3, and then transferred to ex vitro conditions for acclimatization. Plants grown with NH4+ had high mortality. During acclimatization plants cultivated with 30, 60, and 90 mM of N as NH4NO3 showed higher biomass. With regard to N assimilation, GS and NR showed the highest activity in plants cultivated with NH4NO3, whereas plants cultivated with NH4+ had the highest GDH activity. Consequently, in vitro and ex vitro cultivation of this species with 60 mM N as NH4NO3 is recommended.

Influences of exogenous sucrose on juvenile avocado during in vitro cultivation and subsequent ex vitro acclimatization

Trees ◽  
2002 ◽  
Vol 16 (8) ◽  
pp. 569-575 ◽  
Author(s):  
Albert Premkumar ◽  
Araceli Barceló-Muñoz ◽  
Fernando Pliego-Alfaro ◽  
Miguel A. Quesada ◽  
José A. Mercado
Keyword(s):  
In Vitro Cultivation ◽  
Ex Vitro

scholarly journals Embryo culture in vitro in the experimental evaluation of drought resistance in cereals (review)

2021 ◽  
Vol 2(26) ◽  
pp. 127-144
Author(s):  
N.N. Kruglova ◽  
◽  
O.A. Seldimirova ◽  
A.E. Zinatullina ◽  
◽  
...  
Keyword(s):  
Drought Resistance ◽  
Embryo Culture ◽  
Food Resource ◽  
In Vitro Cultivation ◽  
Ex Vitro ◽  
Critical Stage ◽  
Culture In Vitro ◽  
Relative Autonomy ◽  
Stage Of Development

Drought is an unfavorable combination of meteorological conditions when plants experience a long-term water deficiency both in the air and soil. This is one of the most common abiotic stressors, which leads not only to significant crop losses but also rises threat to food security. Researchers are actively developing ways to breed drought-tolerant cultivars of economically valuable crops, especially cereals – the main food resource. One of the promising areas of biotechnological evaluation of the resistance of existing and newly created cereal genotypes to drought for breeding purposes is the use of culture in vitro. In this case, embryos at the particular stage of development are used as explants (so-called embryo culture in vitro). The review aims to analyze the literature and own data on the production of cereal regenerants in embryo culture in vitro under selective experimental conditions of imitation of physiological drought. It has been shown that in vitro cultivation of immature embryos at a critical stage of relative autonomy is especially promising. This kind of embryo does not depend on the physiological factors of the maternal organism and can autonomously give rise to the fully developed plant under adequate conditions in vitro and later ex vitro. This allows the biotechnologist to obtain regenerants directly, excluding an additional time-consuming stage of the formation of morphogenic calli in vitro. As follows, the time required for expensive experiments is also reduced. Data on the identification of the critical stage of the relative autonomy of the cereal embryogenesis are presented. Criterion (proposed by the authors) for identifying this stage by the ability of the embryos to complete embryogenesis and form the seedlings on a hormone-free medium in vitro and give rise to the full developed regenerants ex vitro has been analyzed. Furthermore, the analysis of the laboratory germination of the obtained caryopses was carried out. It was discovered that in spring soft wheat, for example, such stage, corresponding to the formation of all organs in the embryo, occurs 15 days after pollination. The issues of using relatively autonomous embryos in the biotechnological assessment of the genotype drought resistance under selective conditions in vitro are considered.

scholarly journals Paphiopedilum insigne Morphological and Physiological Features during In Vitro Rooting and Ex Vitro Acclimatization Depending on the Types of Auxin and Substrate

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 582
Author(s):  
Monika Poniewozik ◽  
Marzena Parzymies ◽  
Paweł Szot ◽  
Katarzyna Rubinowska
Keyword(s):  
Enzyme Activity ◽  
Chlorophyll Fluorescence ◽  
Water Balance ◽  
Ascorbate Peroxidase ◽  
Morphological Features ◽  
In Vitro Rooting ◽  
Sphagnum Moss ◽  
In Vitro Cultivation ◽  
Ex Vitro

To obtain healthy and good quality plants from in vitro cultivation, it is necessary to produce plantlets with well-developed rooting systems because they must undergo acclimatization, a final and a very difficult stage of micropropagation. In the present research, the effect of auxins NAA, IAA and IBA in concentrations of 0.5; 1; 2.5 and 5 mg·dm−3 on the Paphiopediluminsigne in vitro rooting was studied, and it was noted that 1 mg·dm−3 of IAA or IBA enabled the obtaining of a lot of rooted and good quality plantlets. The subsequent influence of the two most advantageous auxins on the acclimatization of plantlets in different substrates (sphagnum moss, sphagnum moss + substrate for orchids, substrate for orchids, substrate for orchids + acid peat) was tested, in the means of morphological features of plants and their physiological parameters, i.e., chlorophyll fluorescence (FV, Fm, Fv/Fm), stress enzyme activity (catalase, ascorbate peroxidase), and water balance. Considering all the tested features, it might be stated that the best results were obtained when explants were rooted in vitro in the presence of 1 mg·dm−3 of IAA and then planted ex vitro in substrate for orchids.

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

2017 ◽  
Vol 35 (1-2) ◽  
pp. 73-82
Author(s):  
Carlos Iván Espinosa ◽  
Gabriel Ríos
Keyword(s):  
Ex Vitro

El uso de herramientas biotecnológicas como la micropropagación se constituye en una alternativa de reproducción de especies amenazadas y con tamaños poblacionales reducidos. Sin embargo, uno de los problemas críticos en el uso de la micropropagación como herramienta de reproducción es la calidad de las plántulas resultantes en cuanto a su crecimiento y vigor. En el presente trabajo se evalua los efectos de la micropropagación sobre los patrones de crecimiento y sobrevivencia de plán­tulas in vitro de Cinchona officinalis L., una especie que ha sido fuertemente impactada por procesos de tala dentro de bosques naturales durante la época de la colonia. Se realizó un monitoreo de un total de 120 plántulas in vitro y 1988 plántulas ex vitro por 8 meses a partir del último repique. Adi­cionalmente, en cada plántula se contabilizó la cantidad de brotes axilares. Los resultados obtenidos mostraron un efecto remanente de los procesos de micropropagación, los cuales inicialmente inciden en la cantidad de brotes de las plántulas y en el crecimiento; sin embargo, este efecto no influye de forma negativa en la sobrevivencia de las plántulas durante la fase ex vitro

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