Computerized Morphometric Analysis of the Dendrites of Dogiel Type II Neurons

2004 ◽  
Vol 34 (6) ◽  
pp. 639-642
Author(s):  
V. G. Lukashin ◽  
I. N. Zamuraev ◽  
V. N. Chikhman
Keyword(s):  
Morphometric Analysis ◽  
Type Ii ◽  
Dogiel Type Ii

scholarly journals Comparative histochemical and morphometric analysis of muscle fibers of the psoas muscle in individuals of both genders with ageing

2020 ◽  
pp. 107-107
Author(s):  
Vladimir Antic ◽  
Nenad Stojiljkovic ◽  
Milorad Antic
Keyword(s):  
Morphometric Analysis ◽  
Statistical Significance ◽  
Muscle Fibers ◽  
Age Groups ◽  
Psoas Muscle ◽  
Iliopsoas Muscle ◽  
Type I ◽  
Type Ii ◽  
Tissue Samples ◽  
The Right

Background/Aim. In this paper, we analyzed type I and type II muscle fibers of the iliopsoas muscle in persons of both genders with ageing. The aim of this study was to detect the presence and distribution of types I and II muscle fibers in the human psoas muscle using the hematoxylin and eosin method in individuals of different ages and genders. Methods. The material consisted of tissue samples of the right iliopsoas muscle taken from 30 adult cadavers (18 males and 12 females), aged from 30 to 90 years, divided into three age groups. The material was obtained from the Institute of Forensic Medicine, Faculty of Medicine University of Nis. Results. The values of astereological parameters (area, perimeter and Feret's diameter) of type I and type II muscle fibers were higher in male cases, although without any statistical significance. Conclusion. Based on the histochemical and morphometric analysis, the conclusion was drawn that after 70 years of life there occurred a loss of type II muscle fibers, more conspicuous in female cases.

scholarly journals Critical role of 5-HT1A, 5-HT3, and 5-HT7 receptor subtypes in the initiation, generation, and propagation of the murine colonic migrating motor complex

2010 ◽  
Vol 299 (1) ◽  
pp. G144-G157 ◽  
Author(s):  
Eamonn J. Dickson ◽  
Dante J. Heredia ◽  
Terence K. Smith
Keyword(s):  
Critical Role ◽  
Circular Muscle ◽  
Migrating Motor Complex ◽  
Receptor Subtypes ◽  
Type Ii ◽  
Motor Complex ◽  
Intracellular Microelectrodes ◽  
Dogiel Type Ii ◽  
Colonic Migrating Motor Complex

The colonic migrating motor complex (CMMC) is necessary for fecal pellet propulsion in the murine colon. We have previously shown that 5-hydroxytryptamine (5-HT) released from enterochromaffin cells activates 5-HT3 receptors on the mucosal processes of myenteric Dogiel type II neurons to initiate the events underlying the CMMC. Our aims were to further investigate the roles of 5-HT1A, 5-HT3, and 5-HT7 receptor subtypes in generating and propagating the CMMC using intracellular microelectrodes or tension recordings from the circular muscle (CM) in preparations with and without the mucosa. Spontaneous CMMCs were recorded from the CM in isolated murine colons but not in preparations without the mucosa. In mucosaless preparations, ondansetron (3 μM; 5-HT3 antagonist) plus hexamethonium (100 μM) completely blocked spontaneous inhibitory junction potentials, depolarized the CM. Ondansetron blocked the preceding hyperpolarization associated with a CMMC. Spontaneous CMMCs and CMMCs evoked by spritzing 5-HT (10 and 100 μM) or nerve stimulation in preparations without the mucosa were blocked by SB 258719 or SB 269970 (1–5 μM; 5-HT7 antagonists). Both NAN-190 and (S)-WAY100135 (1–5 μM; 5-HT1A antagonists) blocked spontaneous CMMCs and neurally evoked CMMCs in preparations without the mucosa. Both NAN-190 and (S)-WAY100135 caused an atropine-sensitive depolarization of the CM. The precursor of 5-HT, 5-hydroxytryptophan (5-HTP) (10 μM), and 5-carboxamidotryptamine (5-CT) (5 μM; 5-HT1/5/7 agonist) increased the frequency of spontaneous CMMCs. 5-HTP and 5-CT also induced CMMCs in preparations with and without the mucosa, which were blocked by SB 258719. 5-HT1A, 5-HT3, and 5-HT7 receptors, most likely on Dogiel Type II/AH neurons, are important in initiating, generating, and propagating the CMMC. Tonic inhibition of the CM appears to be driven by ongoing activity in descending serotonergic interneurons; by activating 5-HT7 receptors on AH neurons these interneurons also contribute to the generation of the CMMC.

scholarly journals MORPHOMETRIC STUDY OF ISOLATED DOGIEL TYPE II CELLS OF THE INTERMUSCULAR PLEXUS

2021 ◽  
Vol 11 (3) ◽  
pp. 17-19
Author(s):  
Ramilya Babaeva
Keyword(s):  
Small Intestine ◽  
3D Model ◽  
Longitudinal Direction ◽  
3D Models ◽  
Morphometric Study ◽  
Type Ii Cells ◽  
Impregnation Method ◽  
Type Ii ◽  
Type Ii Cell ◽  
Dogiel Type Ii

Aim of the study was to calculate the morphometric parameters of isolated Dogiel type II cells from the intermuscular plexus of the small intestine. Materials and methods: 3D models of the oval-shaped Dogiel type II cells from the intermuscular plexus of a rat (n = 1) were constructed and studied. Neurocytes were identified by the impregnation method on the frontal and horizontal sections of the wall of the small intestine. Results: The results showed that the total number of the nodes in the virtual model was 36534, and the mesh elements — 156595. The resulting 3D model of the cell and nucleus was reduced 900 times to obtain a threedimensional cell and nucleus with absolute dimensions, with a ratio of 1:1 to their true size. The volume of Dogiel type II cell was 2785.11 μm³, the volume of the nucleus was 647.7 μm³ and the volume of its perikaryon was 2785.11 μm³. Conclusion: Dogiel type II cells from the intermuscular plexus of the rat small intestine has an ovoid shaped threedimensional structure. These cells are flattened in transverse direction and elongated in longitudinal direction.

Electrophysiological Features of Morphological Dogiel Type II Neurons in the Myenteric Plexus of Pig Small Intestine

2000 ◽  
Vol 84 (1) ◽  
pp. 102-111 ◽  
Author(s):  
Wim Cornelissen ◽  
Ann De Laet ◽  
Alfons B. A. Kroese ◽  
Pierre-Paul Van Bogaert ◽  
Dietrich W. Scheuermann ◽  
...  
Keyword(s):  
Small Intestine ◽  
Myenteric Plexus ◽  
Type Ii ◽  
Dogiel Type Ii

Species-Dependent Features of Dogiel Type II Neurones in the Mammalian Enteric Nervous System

1999 ◽  
Vol 37 (4-5) ◽  
pp. 241-249 ◽  
Author(s):  
Wim Cornelissen ◽  
Ann de Laet ◽  
Pierre-Paul van Bogaert ◽  
Dietrich W. Scheuermann ◽  
Jean-Pierre Timmermans ◽  
...  
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Type Ii ◽  
Dogiel Type Ii

scholarly journals Classification of human enteric neurons

2021 ◽  
Author(s):  
Axel Brehmer
Keyword(s):  
Enteric Neurons ◽  
Morphological Data ◽  
Type I ◽  
Type Ii ◽  
Functional Heterogeneity ◽  
Structural Investigations ◽  
Innovative Methods ◽  
Further Development ◽  
Dogiel Type Ii

AbstractMajor advances in our understanding of the functional heterogeneity of enteric neurons are driven by the application of newly developed, innovative methods. In contrast to this progress, both animal and human enteric neurons are usually divided into only two morphological subpopulations, “Dogiel type II” neurons (with several long processes) and “Dogiel type I” neurons (with several short processes). This implies no more than the distinction of intrinsic primary afferent from all other enteric neurons. The well-known chemical and functional diversity of enteric neurons is not reflected by this restrictive dichotomy of morphological data. Recent structural investigations of human enteric neurons were performed by different groups which mainly used two methodical approaches, namely detecting the architecture of their processes and target-specific tracing of their axonal courses. Both methods were combined with multiple immunohistochemistry in order to decipher neurochemical codes. This review integrates these morphological and immunohistological data and presents a classification of human enteric neurons which we believe is not yet complete but provides an essential foundation for the further development of human gastrointestinal neuropathology.

Real-Time Morphometric Analysis of Type I and Type II Fibers in Cryostat Sections of Human Muscle Biopsies

1980 ◽  
Vol 166 (2-3) ◽  
pp. 218-238 ◽  
Author(s):  
C.J. Cornelisse ◽  
G.Th.A.M. Bots ◽  
A.R. Wintzen ◽  
J.S. Ploem ◽  
K. van den Broek
Keyword(s):  
Real Time ◽  
Morphometric Analysis ◽  
Human Muscle ◽  
Type I ◽  
Type Ii ◽  
Cryostat Sections ◽  
Muscle Biopsies ◽  
Type Ii Fibers
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