Screening for Arabidopsis mutants affected in the Nii gene expression using the Gus reporter gene

2000 ◽  
Vol 108 (2) ◽  
pp. 161-170 ◽  
Author(s):  
Marie-Thérèse Leydecker ◽  
Isabelle Camus ◽  
Françoise Daniel-Vedele ◽  
Hoai-Nam Truong
Keyword(s):  
Gene Expression ◽  
Reporter Gene ◽  
Arabidopsis Mutants ◽  
Gus Reporter Gene ◽  
Gus Reporter

scholarly journals RETRACTED ARTICLE: The specific MYB binding sites bound by TaMYB in the GAPCp2/3 promoters are involved in the drought stress response in wheat

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Lin Zhang ◽  
Zhiqiang Song ◽  
Fangfang Li ◽  
Xixi Li ◽  
Haikun Ji ◽  
...  
Keyword(s):  
Gene Expression ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Reporter Gene ◽  
Binding Sites ◽  
Promoter Sequence ◽  
Regulatory Elements ◽  
Cis Elements ◽  
Gus Reporter ◽  
Almost All

Abstract Background Drought stress is one of the major abiotic stresses that affects plant growth and productivity. The GAPCp genes play important roles in drought stress tolerance in multiple species. The aim of this experiment was to identify the core cis-regulatory elements that may respond to drought stress in the GAPCp2 and GAPCp3 promoter sequences. Results In this study, the promoters of GAPCp2 and GAPCp3 were cloned. The promoter activities were significantly improved under abiotic stress via regulation of Rluc reporter gene expression, while promoter sequence analysis indicated that these fragments were not almost identical. In transgenic Arabidopsis with the expression of the GUS reporter gene under the control of one of these promoters, the activities of GUS were strong in almost all tissues except the seeds, and the activities were induced after abiotic stress. The yeast one-hybrid system and EMSA demonstrated that TaMYB bound TaGAPCp2P/3P. By analyzing different 5′ deletion mutants of these promoters, it was determined that TaGAPCp2P (− 1312~ − 528) and TaGAPCp3P (− 2049~ − 610), including the MYB binding site, contained enhancer elements that increased gene expression levels under drought stress. We used an effector and a reporter to co-transform tobacco and found that TaMYB interacted with the specific MYB binding sites of TaGAPCp2P (− 1197~ − 635) and TaGAPCp3P (− 1456~ − 1144 and − 718~ − 610) in plant cells. Then, the Y1H system and EMSA assay demonstrated that these MYB binding sites in TaGAPCp2P (− 1135 and − 985) and TaGAPCp3P (− 1414 and − 665) were the target cis-elements of TaMYB. The deletion of the specific MYB binding sites in the promoter fragments significantly restrained the drought response, and these results confirmed that these MYB binding sites (AACTAAA/C) play vital roles in improving the transcription levels under drought stress. The results of qRT-PCR in wheat protoplasts transiently overexpressing TaMYB indicated that the expression of TaGAPCp2/3 induced by abiotic stress was upregulated by TaMYB. Conclusion The MYB binding sites (AACTAAA/C) in TaGAPCp2P/3P were identified as the key cis-elements for responding to drought stress and were bound by the transcription factor TaMYB.

In situ expression of trehalose synthesizing genes, TPS1 and TPPB, in Arabidopsis thaliana using the GUS reporter gene

2009 ◽  
Vol 98 (3) ◽  
pp. 311-319 ◽  
Author(s):  
Hanhong Bae ◽  
Richard Sicher ◽  
Savithiry Natarajan ◽  
Bryan Bailey
Keyword(s):  
Arabidopsis Thaliana ◽  
Reporter Gene ◽  
Gus Reporter Gene ◽  
Gus Reporter

Transient Expression of the GUS Reporter Gene in the Protoplasts and Partially Digested Cells of Ulva lactuca L. (Chlorophyta)

1996 ◽  
Vol 39 (1-6) ◽  
Author(s):  
X. Huang ◽  
J. C. Weber ◽  
T. K. Hinson ◽  
A. C. Mathieson ◽  
S. C. Minocha
Keyword(s):  
Transient Expression ◽  
Reporter Gene ◽  
Ulva Lactuca ◽  
Gus Reporter Gene ◽  
Gus Reporter
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