Antibody Production in a Completely in vitro System

Nature ◽  
1957 ◽  
Vol 179 (4565) ◽  
pp. 870-871 ◽  
Author(s):  
K. M. STEVENS ◽  
J. M. McKENNA
Keyword(s):  
Antibody Production ◽  
In Vitro System

Importance of Cell Division for Antibody Production in an in vitro System

Nature ◽  
1961 ◽  
Vol 192 (4801) ◽  
pp. 462-463 ◽  
Author(s):  
R. W. DUTTON
Keyword(s):  
Cell Division ◽  
Antibody Production ◽  
In Vitro System

scholarly journals STUDIES ON ANTIBODY PRODUCTION

1963 ◽  
Vol 117 (6) ◽  
pp. 1063-1074 ◽  
Author(s):  
Thomas F. O'Brien ◽  
Albert H. Coons
Keyword(s):  
Lymph Node ◽  
Antibody Response ◽  
Antibody Production ◽  
Culture Medium ◽  
Popliteal Lymph Node ◽  
Cell Multiplication ◽  
Measurable Effect ◽  
In Vitro System ◽  
Stimulation Of

Incorporation of 5-bromodeoxyuridine (BUDR) in the culture medium for the 2nd, 3rd, or 4th day after in vitro antigen stimulation of rabbit popliteal lymph node fragments suppressed the in vitro anamnestic antibody response described previously. Before or after this 3-day period, BUDR had no measurable effect. The results suggest that the antibody response in this in vitro system depends upon cell multiplication during this period.

In Vitro Assay of Platelet Adhesiveness with Washed and Tanned Human Red Cells

1968 ◽  
Vol 20 (03/04) ◽  
pp. 384-396 ◽  
Author(s):  
G Zbinden ◽  
S Tomlin
Keyword(s):  
Platelet Adhesion ◽  
Sodium Citrate ◽  
Blood Platelets ◽  
In Vitro Assay ◽  
Red Cells ◽  
Platelet Adhesiveness ◽  
Vitro Assay ◽  
In Vitro System ◽  
Human Red Cells

SummaryAn in vitro system is described in which adhesion of blood platelets to washed and tannic acid-treated red cells was assayed quantitatively by microscopic observation. ADP, epinephrine and TAME produced a reversible increase in platelet adhesiveness which was antagonized by AMP. With Evans blue, polyanetholsulfonate, phthalanilide NSC 38280, thrombin and heparin at concentrations above 1-4 u/ml the increase was irreversible. The ADP-induced increase in adhesiveness was inhibited by sodium citrate, EDTA, AMP, ATP and N-ethylmaleimide. EDTA, AMP and the SH-blocker N-ethylmaleimide also reduced spontaneous platelet adhesion to red cells. No significant effects were observed with adenosine, phenprocoumon, 5-HT, phthalanilide NSC 57155, various estrogens, progestogens and fatty acids, acetylsalicylic acid and similarly acting agents, hydroxylamine, glucose and KCN. The method may be useful for the screening of thrombogenic and antithrombotic properties of drugs.

The Mesothelial Cell as a Non-Thrombogenic Surface

1984 ◽  
Vol 52 (02) ◽  
pp. 102-104 ◽  
Author(s):  
L J Nicholson ◽  
J M F Clarke ◽  
R M Pittilo ◽  
S J Machin ◽  
N Woolf
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Blood Flow ◽  
Cell Surface ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Plastic Film ◽  
In Vitro System ◽  
Scanning Electron

SummaryA technique for harvesting mesothelial cells is described. This entails collagenase digestion of omentum after which the cells can be cultured. The technique has been developed using the rat, but has also been successfully applied to human tissue. Cultured rat mesothelial cells obtained in this way have been examined by scanning electron microscopy. Rat mesothelial cells grown on plastic film have been exposed to blood in an in vitro system using a Baumgartner chamber and have been demonstrated to support blood flow. No adhering platelets were observed on the mesothelial cell surface. Fibroblasts similarily exposed to blood as a control were washed off the plastic.

scholarly journals Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Carlota Oleaga ◽  
Catia Bernabini ◽  
Alec S.T. Smith ◽  
Balaji Srinivasan ◽  
Max Jackson ◽  
...  
Keyword(s):  
In Vitro System ◽  
Organ Toxicity

scholarly journals Evaluation of the bioaccessibility of a carotenoid beadlet blend using an in vitro system mimicking the upper gastrointestinal tract

2021 ◽  
Author(s):  
Chun Hu ◽  
Dawna Salter Venzon ◽  
Katja Lange ◽  
Annet Maathuis ◽  
Susann Bellmann ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Upper Gastrointestinal Tract ◽  
In Vitro System ◽  
Upper Gastrointestinal
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