Gonadal Development and Reproductive Activity of the Cichlid Fish Tilapia leucosticta (Trewavas) in an Equatorial Lake

Sex hormones play an essential role in sexual differentiation, maintenance of sexual characteristics, gamete maturation, and mating behavior. However, very little is known about their dynamics in molluscs. We conducted a study on sex hormone (17β-estradiol, testosterone, and progesterone) concentrations in male and female Strombus pugilis to identify their variations at different gonadal stages. A total of 90 organisms (30 per month) were collected in February, September, and November 2016. The gonadal digestive gland complex of each specimen was dissected and divided into two sections. One section was set in alcoholic Bouin's fluid and processed with classic histological techniques; the second was macerated with 80% ethanol to extract steroids and analyzed by enzyme immunoassays. Histological section analysis was used to classify gonadal development into three stages: gametogenesis, mature, and undifferentiated. Mature females were observed in September. Testosterone and 17β-estradiol concentrations in both sexes were highest in the mature stage. In S. pugilis, 17β-estradiol, progesterone, and testosterone were all present, with higher concentrations associated with reproductive activity.

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Reproductive ecology of a neotropical cichlid fish, Cichla monoculus (Osteichthyes: Cichlidae)

Brazilian Journal of Biology ◽

10.1590/s1519-69842003000100004 ◽

2003 ◽

Vol 63 (1) ◽

pp. 17-26 ◽

Cited By ~ 32

Author(s):

S. Chellappa ◽

M. R. Câmara ◽

N. T. Chellappa ◽

M. C. M. Beveridge ◽

F. A. Huntingford

Keyword(s):

Reproductive Ecology ◽

Cichlid Fish ◽

Reproductive Activity ◽

Reproductive Capacity ◽

Northeast Brazil ◽

Monthly Basis ◽

Study Region ◽

Frequency Distributions ◽

Multiple Spawner ◽

Semi Arid

The reproductive ecology of the freshwater fish Cichla monoculus Spix, 1831 (Osteichthyes: Cichlidae) was investigated in the Campo Grande Reservoir, Northeast Brazil. Rainfall, temperature, pH, dissolved oxygen and electrical conductivity of the water were recorded monthly. Fish collected on a monthly basis were measured, weighed, dissected, sexed and the stage of maturation of the gonads were assessed by macro and microscopic means. The semi-arid study region has short spells of rain of 2-3 months duration interspersed with dry seasons. A positive correlation was observed between rainfall and air and water temperatures and conductivity of the water. The study population had an extended spawning season, with peak reproductive activity coinciding with low water temperatures. Males were longer and heavier than females on average and were larger at onset of sexual maturity. The size frequency distributions of the oocytes indicate that C. monoculus is a multiple spawner with an estimated batch fecundity of 3100. Condition factor showed an inverse relationship in relation to gonad size during maturation in both sexes and spent fish were in poor condition. In mature males, lipid stores in the post-occipital cephalic protuberance, a secondary sexual characteristic developed during the reproductive phase, which depleted in spent individuals. The success of this fish is attributed to its reproductive capacity and to the phenotypic plasticity that allows it to adapt to the harsh ecological conditions that prevail in the semi-arid region of Northeast Brazil.

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Reproductive biology of Hepatus pudibundus (Crustacea: Brachyura), the most abundant crab on the southeastern Brazilian coast

Biologia ◽

10.2478/s11756-013-0301-4 ◽

2014 ◽

Vol 69 (2) ◽

Cited By ~ 2

Author(s):

Paloma Aparecida de Lima ◽

Giovana Bertini ◽

Vivian Fransozo ◽

Rafael Gregati ◽

Lissandra Corrêa Fernandes-Góes ◽

...

Keyword(s):

Bottom Water ◽

Carapace Width ◽

Sampling Period ◽

Reproductive Activity ◽

Gonadal Development ◽

Reproductive Period ◽

Brazilian Coast ◽

Northern Coast ◽

Adult Males ◽

Bottom Water Temperature

AbstractThis study analyzed the size at sexual maturity and reproductive period of populations of Hepatus pudibundus in three bays on the northern coast of São Paulo, Brazil. Crabs were collected monthly and the bottom-water temperature was measured at each collection point. The animals were sexed, measured for carapace width (CW), and their gonadal stages were determined. A total of 8,674 specimens were collected (2,435 males and 6,239 females). Adult males showed the highest mean CW; the size at maturity for both sexes was 32.5 mm CW. Reproduction was continuous and peaked in spring and summer, because of the greater availability of plankton food for the larvae. This pattern is typical in tropical and subtropical regions, unlike the seasonal reproduction found in temperate regions. Reproductive activity of females was not significantly correlated with bottom-water temperatures. Immatures and individuals in all stages of gonadal development were found throughout the sampling period and at all depths, probably because the species completes its entire reproductive cycle in that area.

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Temporal Dynamics of Reproduction of the Neotropical Fish,Crenicichla menezesi(Perciformes: Cichlidae)

The Scientific World JOURNAL ◽

10.1100/2012/579051 ◽

2012 ◽

Vol 2012 ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Andréa Soares de Araújo ◽

Wallace Silva do Nascimento ◽

Maria Emília Yamamoto ◽

Sathyabama Chellappa

Keyword(s):

Sexual Maturity ◽

Temporal Dynamics ◽

Condition Factor ◽

Cichlid Fish ◽

Gonadal Development ◽

Neotropical Fish ◽

Spawning Period ◽

Gonadal Maturation ◽

Development Cycle ◽

Partial Spawner

The reproductive biology and the gonadal development cycle of the Neotropical cichlid fish,Crenicichla menezesi, is described. This species exhibits sexual dimorphism only during the spawning season. First sexual maturity of females is attained earlier than the males. Both macroscopic and histological investigations of ovaries and testes revealed four stages of gonadal maturation. Mean batch fecundity of females was 372 (±10,41) of mature oocytes. This species is a partial spawner, with an extended spawning period. Monthly values of GSI and the condition factor are negatively correlated during the gonadal development cycle of this species.

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Cellular development of the germinal epithelium during the female and male gametogenesis of Chaetodon striatus (Perciformes: Chaetodontidae)

Zygote ◽

10.1017/s0967199420000118 ◽

2020 ◽

Vol 28 (4) ◽

pp. 291-299

Author(s):

Isabelle Leite Bayona Perez ◽

Talita Sarah Mazzoni ◽

Irani Quagio-Grassiotto

Keyword(s):

Reproductive Activity ◽

Gonadal Development ◽

Reproductive Period ◽

Morphological Data ◽

Germinal Epithelium ◽

Cellular Dynamics ◽

Spawning Period ◽

Reproductive Life ◽

Early Primary ◽

Reproductive Phases

SummaryButterflyfish Chaetodon striatus is highly sought after in the marine ornamental aquarium, although studies about its reproductive biology are scarce. Therefore, to contribute to a better understanding of the reproductive aspects of C. striatus, we describe in detail with the use of high resolution histology the cellular dynamics of the germinal epithelium during the reproductive life history of this species. Based on the activity of the germinal epithelium, this study describes different stages of the gonadal development, similar to the reproductive phases found in other fish, to determine the reproductive period of C. striatus. In characterization of gonadal development, the following germ cells are described for males: spermatogonia, spermatocytes, spermatids and spermatozoa. Oogonia, early, primary, secondary, full-grown and maturing oocytes are described for females. Female germinal epithelium of C. striatus showed substantial changes over the study period, indicating that there was an active spawning period. Male germinal epithelium also presented relevant alterations, indicating reproductive activity in the testicular lobules. Morphological data confirm how informative was the cellular dynamics of the germinal epithelium for understanding gonadal development during adult reproductive life of fish in general. Although Chaetodon are a popular species, previous studies have only produced superficial and rough histological analyses. Therefore, this study demonstrates important information on germinal epithelium of Chaetodon. This knowledge could be a fundamental tool for development of new strategies for breeding of several species in captivity, especially butterflyfishes.

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INDUKSI PEMATANGAN GONAD IKAN GURAMI COKELAT (Sphaerichthys osphromenoides Canestrini, 1860) MENGGUNAKAN PREGNANT MARE SERUM GONADOTROPIN DAN ANTIDOPAMIN

Jurnal Riset Akuakultur ◽

10.15578/jra.12.1.2017.69-76 ◽

2017 ◽

Vol 12 (1) ◽

pp. 69

Author(s):

Bastiar Nur ◽

Sawung Cindelaras ◽

Nina Meilisza

Keyword(s):

Body Weight ◽

Fish Species ◽

Gonadosomatic Index ◽

Gonad Development ◽

Stage Iv ◽

Reproductive Activity ◽

Gonadal Development ◽

Pregnant Mare Serum Gonadotropin ◽

Gonad Somatic Index ◽

Serum Gonadotropin

Ikan gurami cokelat (Sphaerichthys osphromenoides Canestrini, 1860) merupakan salah satu spesies ikan hias endemik perairan gambut dan memiliki potensi untuk dibudidayakan. Pada kondisi budidaya, berbagai faktor lingkungan yang berperan penting dalam menstimulasi perkembangan dan pematangan gonad hingga ovulasi dan pemijahan tidak mendukung aktivitas reproduksi beberapa spesies ikan. Penelitian ini dilakukan untuk menentukan dosis hormon yang efektif dapat merangsang pematangan gonad ikan gurami cokelat. Hormon yang digunakan adalah “Oodev®” (tersusun atas Pregnant Mare Serum Gonadotropin/PMSG) dan antidopamin) yang diberikan menggunakan metode “topical gill”. Ada tiga dosis Oodev® yang digunakan, yaitu: 0,02 mL; 0,04 mL; dan 0,06 mL; serta kontrol menggunakan NaCl 0,9% sebanyak 0,05 mL/g bobot badan ikan uji. Setiap perlakuan menggunakan 30 ekor induk betina ikan gurami cokelat (panjang total: 4,1 ± 0,3 cm; bobot: 1,41 ± 0,17 g). Pemberian hormon dilakukan setiap minggu hingga minggu ke-7. Pada minggu ke-8 dilakukan pembedahan untuk pengambilan gonad. Parameter yang diamati adalah: jumlah induk matang gonad, indeks gonadosomatik (IGS), fekunditas, diameter oosit, kadar estradiol-17â dalam darah, dan tingkat kematangan gonad (TKG) ikan uji pada masing-masing perlakuan. Hasil penelitian menunjukkan bahwa perlakuan hormon dengan dosis 0,04 mL/g bobot badan menghasilkan perkembangan gonad ikan uji yang lebih baik dengan jumlah induk matang gonad mencapai 23 ekor (76,67%), nilai IGS sebesar 2,33 ± 1,24%; fekunditas sebesar 73,5 ± 26,2 butir; diameter oosit berkisar antara 1,0-1,8 mm; kadar estradiol-17b dalam darah sebesar 15,9 ± 4,5 rg/mL dengan tingkat kematangan gonad mencapai tahap IV.Chocolate gourami (Sphaerichthys osphromenoides Canestrini, 1860) is an endemic ornamental fish species in peatland waters and potentially to be cultivated. In captive condition, some environmental factors that play important role for gonadal development, maturation, ovulation, and spawning are not suitable for supporting reproductive activity in some fish species. This study was conducted to determine the optimum dosages of hormone which is able to stimulate gonadal maturation of chocolate gourami. Oodev® (consisted of Pregnant Mare Serum Gonadotropin (PMSG) and antidopamin) was given using “topical gill” method. There were three Oodev® dosages used in this research: 0.02 mL; 0.04 mL; and 0.06 mL respectively; and control using 0.05 mL of 0.9% NaCl per gram of body weight. Each treatment was tested on 30 females (the averages of total length and body weight of 4.1 ± 0.3 cm and 1.41 ± 0.17 g, respectively). Hormone was given every week until the seventh week. At the eight weeks, surgery was performed for gonadal measurement. Parameters measured were: number of gonadal mature broodstocks, gonadosomatic index, fecundity, oocyte diameters, plasma estradiol-17â levels, and gonadal mature levels. The results showed that 0.04 mL Oodev® per g body weight of fish was superior in reproductive performance. In that treatment, there were 76.67% (23/30) fish being matured, gonad somatic index 2.33 ± 1.24%, egg fecundity 73.5 ± 26.2 eggs, oocyte diameters ranged 1.0-1.8 mm, blood level of estradiol-17b 15.9 ± 4.5 rg.mL-1, and the level of gonad development reached to stage IV.

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