Cellular development of the germinal epithelium during the female and male gametogenesis of Chaetodon striatus (Perciformes: Chaetodontidae)

Zygote ◽  
2020 ◽  
Vol 28 (4) ◽  
pp. 291-299
Author(s):  
Isabelle Leite Bayona Perez ◽  
Talita Sarah Mazzoni ◽  
Irani Quagio-Grassiotto
Keyword(s):  
Reproductive Activity ◽  
Gonadal Development ◽  
Reproductive Period ◽  
Morphological Data ◽  
Germinal Epithelium ◽  
Cellular Dynamics ◽  
Spawning Period ◽  
Reproductive Life ◽  
Early Primary ◽  
Reproductive Phases

SummaryButterflyfish Chaetodon striatus is highly sought after in the marine ornamental aquarium, although studies about its reproductive biology are scarce. Therefore, to contribute to a better understanding of the reproductive aspects of C. striatus, we describe in detail with the use of high resolution histology the cellular dynamics of the germinal epithelium during the reproductive life history of this species. Based on the activity of the germinal epithelium, this study describes different stages of the gonadal development, similar to the reproductive phases found in other fish, to determine the reproductive period of C. striatus. In characterization of gonadal development, the following germ cells are described for males: spermatogonia, spermatocytes, spermatids and spermatozoa. Oogonia, early, primary, secondary, full-grown and maturing oocytes are described for females. Female germinal epithelium of C. striatus showed substantial changes over the study period, indicating that there was an active spawning period. Male germinal epithelium also presented relevant alterations, indicating reproductive activity in the testicular lobules. Morphological data confirm how informative was the cellular dynamics of the germinal epithelium for understanding gonadal development during adult reproductive life of fish in general. Although Chaetodon are a popular species, previous studies have only produced superficial and rough histological analyses. Therefore, this study demonstrates important information on germinal epithelium of Chaetodon. This knowledge could be a fundamental tool for development of new strategies for breeding of several species in captivity, especially butterflyfishes.

scholarly journals Cellular development of the germinal epithelium during the gametogenic cycle of the golden mussel Limnoperna fortunei (Bivalvia: Mytilidae)

2016 ◽  
Vol 64 (2) ◽  
pp. 521 ◽  
Author(s):  
Claudenice Dei Tos ◽  
Irani Quagio Grassiotto ◽  
Talita Sarah Mazzoni
Keyword(s):  
Invasive Species ◽  
Control Strategies ◽  
Morphological Data ◽  
Germinal Epithelium ◽  
Early Prophase ◽  
Natural Environments ◽  
Limnoperna Fortunei ◽  
Reproductive Life ◽  
Reproductive Phases ◽  
Golden Mussel

<p>The golden mussel <em>Limnoperna fortunei</em> is an invasive species that has quickly dispersed and colonized several potential different habitats distributed all over the world, causing environmental and economic impacts. The control of the species depends on knowledge of its reproductive aspects. Approximately 1 200 specimens of <em>L. fortunei</em> were sampled periodically on the upper Paraná River floodplain (Brazil) from March 2010 to December 2012. Thus, in order to contribute to a better understanding of the reproductive aspects of <em>L. fortunei</em>, using high resolution histology, we described the cellular dynamic of the male and female germinal epithelium during the annual reproductive life history of this species. Based on the activity of the germinal epithelium and consequent germ cell development, this study has resulted in the recognition of the following reproductive phases: Developing, Spawning Capable, Regressing and Regenerating. In the characterization of these phases the following germ cells were described for males: spermatogonia, primary and secondary spermatocytes, spermatids and spermatozoa. Cell nests, oogonia, early prophase oocytes, previtellogenic oocytes and vitellogenic oocytes (early vitellogenic oocytes, middle vitellogenic oocytes and full-grown oocytes) were described for females. The morphological data and reproductive parameters shown here confirm how informative the cellular dynamic of the germinal epithelium is for the understanding of the cyclic gonadal events during the adult reproductive life of the mollusk in general. These knowledge about the gametogenesis of invasive species can become a fundamental tool for the development of control strategies and implementation of programs to decrease their proliferation in natural environments.</p>

Reproductive biology of Hepatus pudibundus (Crustacea: Brachyura), the most abundant crab on the southeastern Brazilian coast

Biologia ◽  
2014 ◽  
Vol 69 (2) ◽  
Author(s):  
Paloma Aparecida de Lima ◽  
Giovana Bertini ◽  
Vivian Fransozo ◽  
Rafael Gregati ◽  
Lissandra Corrêa Fernandes-Góes ◽  
...  
Keyword(s):  
Bottom Water ◽  
Carapace Width ◽  
Sampling Period ◽  
Reproductive Activity ◽  
Gonadal Development ◽  
Reproductive Period ◽  
Brazilian Coast ◽  
Northern Coast ◽  
Adult Males ◽  
Bottom Water Temperature

AbstractThis study analyzed the size at sexual maturity and reproductive period of populations of Hepatus pudibundus in three bays on the northern coast of São Paulo, Brazil. Crabs were collected monthly and the bottom-water temperature was measured at each collection point. The animals were sexed, measured for carapace width (CW), and their gonadal stages were determined. A total of 8,674 specimens were collected (2,435 males and 6,239 females). Adult males showed the highest mean CW; the size at maturity for both sexes was 32.5 mm CW. Reproduction was continuous and peaked in spring and summer, because of the greater availability of plankton food for the larvae. This pattern is typical in tropical and subtropical regions, unlike the seasonal reproduction found in temperate regions. Reproductive activity of females was not significantly correlated with bottom-water temperatures. Immatures and individuals in all stages of gonadal development were found throughout the sampling period and at all depths, probably because the species completes its entire reproductive cycle in that area.

scholarly journals Reproductive Ecology ofProchilodus brevisan Endemic Fish from the Semiarid Region of Brazil

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Liliane de Lima Gurgel ◽  
José Roberto Verani ◽  
Sathyabama Chellappa
Keyword(s):  
Gonadosomatic Index ◽  
Reproductive Ecology ◽  
Temporal Distribution ◽  
Gonadal Development ◽  
Neotropical Region ◽  
Reproductive Period ◽  
Semiarid Region ◽  
Spawning Period ◽  
Gonadal Maturity ◽  
Commercially Important

The commercially important migratory fishProchilodus brevisis from the Neotropical region, and understanding the reproductive ecology of this potamodromous fish is essential for its conservation and management. This study investigated the length-mass relationship, sex ratio, length at first gonadal maturity, gonadal development stages, gonadosomatic index, condition factor, and reproductive period ofP. brevis. Temporal distribution of rainfall, temperature, dissolved oxygen concentration, pH, and electrical conductivity of the water were related to the reproductive period of this fish. Rainfall seems to be the main environmental factor which modulates changes in limnological parameters and the timing of the spawning period of this fish.P. brevismigrates into lower reaches of the river to feed during the dry season and returns to the upper reaches during the rainy season to spawn. Inadequate facilities for migration create obstacles for spawning success of this ecologically important fish.

scholarly journals Gonadal development and reproductive period of the characin Astyanax aff. bimaculatus (Characiformes: Characidae) in a tropical reservoir in southeastern Brazil

2019 ◽  
Vol 36 ◽  
pp. 1-14
Author(s):  
Francisco G. Araújo ◽  
Aparecida A. Nascimento ◽  
Iracema D. Gomes ◽  
Armando Sales ◽  
Beatriz A. Chagas de Oliveira
Keyword(s):  
Gonadosomatic Index ◽  
Gonadal Development ◽  
Neotropical Region ◽  
Reproductive Period ◽  
Southeastern Brazil ◽  
Energy Reserves ◽  
Tropical Reservoir ◽  
Spawning Period ◽  
Wide Range ◽  
Body Energy

The gonadal development of the characin Astyanax aff. bimaculatus from Funil Reservoir, an impoundment in the middle reaches of the Paraiba do Sul River in southeastern Brazil was analysed through histological, histochemical and histometric techniques, and the spawning season was determined. Unbalanced sex ratio was found, with females outnumbering and reaching larger sizes than males. The following stages of oogenic cell development were identified for females: primary growth, pre-vitellogenic (cortical alveolar) and vitellogenic. In males, the following stages were determined: spermatogonia primary and secondary, spermatocyte primary and secondary, spermatid and spermatozoa. The spawning occurs most of the year, peaking in January-February, as indicated by the gonadosomatic index. In the peak of the spawning period, the condition factor and hepatosomatic indices decreased suggesting use of stored body energy reserves during the reproduction. Spawning in batches, small-sized oocytes and a long reproductive season suggest that Astyanax aff. bimaculatus fits to an opportunist strategy. Such strategy may facilitate the use of a wide range of lentic and lotic body waters in the Neotropical region.

CYCLIC CHANGES IN THE GONAD OF THE AMERICAN OYSTER, CRASSOSTREA VIRGINICA (GMELIN)

1964 ◽  
Vol 42 (2) ◽  
pp. 305-321 ◽  
Author(s):  
Austina V. Kennedy ◽  
Helen I. Battle
Keyword(s):  
Crassostrea Virginica ◽  
Gonadal Development ◽  
Germinal Epithelium ◽  
Nuclear Area ◽  
Seasonal Growth ◽  
Body Width ◽  
Visceral Mass ◽  
Current Season ◽  
Primary Oocyte ◽  
Cyclic Changes

Cyclic changes in the gonad of Crassostrea virginica (Gmelin), a dioecious, oviparous lamellibranch, are described as they occurred toward the most northerly limit of the range, Malpeque Bay, P.E.I., Canada, during 1961 and 1962. The gonad, composed of right and left gonadal lobes lying immediately beneath the mantle, consists of extensively branched follicles comprising the outer margin of the visceral mass. The follicles open into peripherally located ducts which lead into paired gonoducts terminating in the suprabranchial chamber. During the fall and winter the germinal epithelium is in an indifferent or inactive state. The sex for the current season is distinguishable when proliferation commences in May. Maximum gonadal development occurs in late June or early July as determined by comparison of gonadal width to body width in mid-transverse sections. Primary oocytes are initially distinguishable from oogonia by the presence of a distinct nucleolus, and later by an amphinucleolus consisting of a plasmosome and a karyosome. Seasonal growth of the primary oocyte was followed by a planimetry method using measurements of total area and nuclear area from prepared sections. The spindle for the first meiotic division is established immediately on rupture of the oocyte from the follicular wall. Spermatogenesis and spermiogenesis are completed within the follicle. Following spawning, amoebocytes infiltrate the follicles and interfollicular connective tissue to phagocytize unspawned gametes. By late October the follicles of both male and female consist of a low germinal epithelium and a few unresorbed gametes, and remain inactive until proliferation the following spring.

Reproductive biology of the dusky grouper (Epinephelus marginatus) at the southern limit of its distribution in the south-western Atlantic

2014 ◽  
Vol 65 (2) ◽  
pp. 142 ◽  
Author(s):  
Mario V. Condini ◽  
Luís F. Fávaro ◽  
Antonio S. Varela ◽  
Alexandre M. Garcia
Keyword(s):  
Reproductive Activity ◽  
The South ◽  
Western Atlantic ◽  
Spawning Period ◽  
Southern Limit ◽  
Rocky Reefs ◽  
Dusky Grouper ◽  
First Maturity ◽  
Rocky Bottoms ◽  
Partial Spawner

We investigated the reproductive parameters of a dusky grouper Epinephelus marginatus population at the southern limit of its distribution in the south-western Atlantic. The analysed specimens were captured between January 2008 and April 2011 at Carpinteiro Bank (CB) (32°16′S; 051°47′W), a seamount located at the 20 m isobath ~16 nautical miles from the coast. The gonads of a total of 201 individuals (184 females, 16 males and 1 individual in sexual transition) with a total length (TL, mm) between 278 and 1160 mm were analysed microscopically. Histological analyses of the gonads suggested that the species is a partial spawner with a long spawning period (between November and March) and with a reproductive peak between November and January. The average female length at first maturity (L50) was 496 (TL, mm). We also observed partially spawned ovaries and partially spawned testes, which indicated reproductive activity, and we noted the presence of hyalinised follicles. These findings reveal that CB is the southernmost portion of the south-western Atlantic in which the dusky grouper reproduces. Additionally, an inshore-offshore comparison suggested that deeper (>20 m) rocky bottoms, such as the studied seamount, constitute higher quality habitats for this species when compared with littoral (<5 m) rocky reefs.

scholarly journals Population structure and reproductive biology of Cichla kelberi (Perciformes, Cichlidae) in Lobo Reservoir, Brazil

2008 ◽  
Vol 6 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Jussara E. de Souza ◽  
Evelise N. Fragoso-Moura ◽  
Nelsy Fenerich-Verani ◽  
Odete Rocha ◽  
José R. Verani
Keyword(s):  
Sex Ratio ◽  
Condition Factor ◽  
Reproductive Activity ◽  
Reproductive Period ◽  
High Fecundity ◽  
Long Period ◽  
Total Length ◽  
Relative Condition Factor ◽  
Central Value ◽  
Large Output

The structure of Cichla kelberi population was characterized regarding the sex ratio, total length composition and some reproductive characteristics. The specimens were collected in two periods, from November 2002 to November 2003 and from December 2004 to May 2006. The sex ratio was significantly different than 1:1. The total length varied from 3.4 to 40.1 cm, with individuals in the shortest length classes occurring throughout the year, but with greater incidence in the warmer periods. Females and males reached similar lengths and there was no difference between the sexes regarding the length-weight relationship. The estimated values of condition factor were near the central value of 1.0 and decreased during the cooler months, coinciding with periods of lower reproductive activity. Individuals with mature or semi-spent gonads occurred from November to April, indicating that Cichla kelberi reproduces for a long period of time. The spawning is partitioned, with an average absolute fecundity of 6072 oocytes (from 5220 to 6908 oocytes). The high relative condition factor values, long reproductive period, large output of fingerlings and high fecundity are biological conditions that indicate the species is well adapted in this reservoir.

Sex hormones in Strombus pugilis (Mollusca: Gastropoda) in different gonadal stages

2020 ◽  
Vol 96 (4) ◽  
pp. 679-694
Author(s):  
Fabiola Chong Sánchez ◽  
Martha Enríquez Díaz ◽  
Dalila Aldana Aranda
Keyword(s):  
Sex Hormones ◽  
Histological Section ◽  
Reproductive Activity ◽  
Gonadal Development ◽  
Mature Stage ◽  
Histological Techniques ◽  
Sexual Characteristics ◽  
17Β Estradiol ◽  
Three Stages ◽  
Section Analysis

Sex hormones play an essential role in sexual differentiation, maintenance of sexual characteristics, gamete maturation, and mating behavior. However, very little is known about their dynamics in molluscs. We conducted a study on sex hormone (17β-estradiol, testosterone, and progesterone) concentrations in male and female Strombus pugilis to identify their variations at different gonadal stages. A total of 90 organisms (30 per month) were collected in February, September, and November 2016. The gonadal digestive gland complex of each specimen was dissected and divided into two sections. One section was set in alcoholic Bouin's fluid and processed with classic histological techniques; the second was macerated with 80% ethanol to extract steroids and analyzed by enzyme immunoassays. Histological section analysis was used to classify gonadal development into three stages: gametogenesis, mature, and undifferentiated. Mature females were observed in September. Testosterone and 17β-estradiol concentrations in both sexes were highest in the mature stage. In S. pugilis, 17β-estradiol, progesterone, and testosterone were all present, with higher concentrations associated with reproductive activity.

Evaluation of Different eCG Doses + Progesterone to Induce Reproductive Activity During the Transitional Reproductive Season in Anestrous Creole Goats

2020 ◽  
Author(s):  
F. G. Véliz-Deras ◽  
S. Zúñiga-García ◽  
G. Calderón-Leyva ◽  
J. Otal-Salaverri ◽  
S. Moreno-Avalos ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Reproductive Behavior ◽  
Reproductive Activity ◽  
Reproductive Period ◽  
Reproductive Season ◽  
Reproductive Seasonality ◽  
Experimental Protocols ◽  
Equine Chorionic Gonadotropin ◽  
Experimental Treatments ◽  
Different Doses

Background: Goats from subtropical and temperate latitudes show reproductive seasonality. For this reason, the products obtained from goats also shows the same seasonality, reducing the finances of goat keepers. Several studies have focused on reducing reproductive seasonality through the use of hormonal protocols based on progestogens, in addition to the use of equine chorionic gonadotropin (eCG) hormone, used for its double biological activity that ensures an optimal response to induce estrus and pregnancy in goats. The objective was to assess whether reduced doses of eCG are effective in inducing reproductive activity in anestrous goats. Methods: During the transition reproductive period (June), mix-breed Creole goats (n=39), were treated with intramuscular progesterone (P4), later, the experimental treatments, consisting in different doses (50, 100, 50+50 or 200 IU) of equine chorionic gonadotropin (eCG) were applied. After the application of the experimental protocols, the reproductive activity of the goats was evaluated. Result: This study shows that one dose of 100 IU of eCG effectively induced reproductive behavior in anestrous Creole goats towards the end of the seasonal anestrus. This protocol makes the use of exogenous hormones more efficient, with reduce doses, decreasing expenses and is practical use by goat producers.

scholarly journals Seasonal changes in size, sex-ratio and body condition of the damselfishChromis chromisin the central Mediterranean Sea

2014 ◽  
Vol 94 (5) ◽  
pp. 1053-1061 ◽  
Author(s):  
Claudia Bracciali ◽  
Susanna Piovano ◽  
Gianluca Sarà ◽  
Cristina Giacoma
Keyword(s):  
Population Structure ◽  
Sex Ratio ◽  
Body Condition ◽  
Seasonal Changes ◽  
Marine Protected Area ◽  
Reproductive Period ◽  
Rocky Shores ◽  
Central Mediterranean ◽  
Monthly Basis ◽  
Spawning Period

Chromis chromisis one of the most abundant fish species on the Mediterranean rocky shores. Acting as a by-pass of nutrients from the pelagic to the rocky littoral system, it plays an important role as a resource provider and has a relevant function in the community assemblage.Chromis chromishas many of the characteristics required of an indicator species (e.g. it is easy to recognize in the field, small sized, widespread and abundant). In this study we focused on individual size, sex-ratio and body condition in a damselfish population occurring in a central Mediterranean marine protected area. Fishing trials on a monthly basis (April–November) allowed us to assess seasonal changes in population structure. Fish were measured and weighed and a subsample was aged and sexed. On average, males were larger than females. The allometric coefficient,b, for the whole population was 2.99; the maximum age recorded was 7.5 yr. Before reproduction sex-ratio was close to parity (M:F 1:0.78), while a larger proportion of females was captured during the reproductive period. In conclusion, our results show that the population dynamics ofC. chromisis influenced by both sex and size. Since males and females have different behavioural strategies, and since the length of the spawning period is shorter in the smaller than in the larger-sized individuals, this is reflected in temporal changes observed in the population structure.

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